A5082330841- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Advanced Fluorescence Microscopy Techniques
- Virus-based gene therapy research
- Polyomavirus and related diseases
- Bacteriophages and microbial interactions
- DNA Repair Mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Bacterial Genetics and Biotechnology
- Monoclonal and Polyclonal Antibodies Research
- Advanced biosensing and bioanalysis techniques
- Animal Genetics and Reproduction
- Ubiquitin and proteasome pathways
- Developmental Biology and Gene Regulation
- Protein Degradation and Inhibitors
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- Cancer-related gene regulation
- Retinoids in leukemia and cellular processes
- Single-cell and spatial transcriptomics
- SARS-CoV-2 detection and testing
- Pluripotent Stem Cells Research
Howard Hughes Medical Institute
2016-2025
University of California, Berkeley
2016-2025
California Institute for Regenerative Medicine
2014-2024
New York Academy of Sciences
2022-2024
John Wiley & Sons (Germany)
2022-2024
Moss Landing Marine Laboratories
2022-2024
Hudson Institute
2022-2024
Middle East Institute
2024
Liechtenstein Institute
2024
Municipality of Medellín
2023
The biochemical analysis of cellular trans-activators involved in promoter recognition provides an important step toward understanding the mechanisms gene expression animal cells. selective transcription factor, Sp1, has been purified from human cells to more than 95 percent homogeneity by sequence-specific DNA affinity chromatography. Isolation and renaturation proteins sodium dodecyl sulfate polyacrylamide gels allowed identification two polypeptides (105 kilodaltons) as those responsible...
We describe a method for affinity purification of sequence-specific DNA binding proteins that is fast and effective. Complementary chemically synthesized oligodeoxynucleotides contain recognition site protein are annealed ligated to give oligomers. This then covalently coupled Sepharose CL-2B with cyanogen bromide yield the resin. A partially purified fraction combined competitor subsequently passed through DNA-Sepharose The desired because it preferentially binds sites in resin rather than...
Choanoflagellates are the closest known relatives of metazoans. To discover potential molecular mechanisms underlying evolution metazoan multicellularity, we sequenced and analysed genome unicellular choanoflagellate Monosiga brevicollis. The contains approximately 9,200 intron-rich genes, including a number that encode cell adhesion signalling protein domains otherwise restricted to Here show physical linkages among often differ between M. brevicollis metazoans, suggesting abundant domain...
Phase separation and gene control Many components of eukaryotic transcription machinery—such as factors cofactors including BRD4, subunits the Mediator complex, RNA polymerase II—contain intrinsically disordered low-complexity domains. Now a conceptual framework connecting nature behavior their interactions to functions in regulation is emerging (see Perspective by Plys Kingston). Chong et al. found that domains form concentrated hubs via functionally relevant dynamic, multivalent,...
TFIID is a large multiprotein complex that initiates assembly of the transcription machinery. It unclear how recognizes promoters in vivo when templates are nucleosome-bound. Here, it shown TAFII250, largest subunit TFIID, contains two tandem bromodomain modules bind selectively to multiply acetylated histone H4 peptides. The 2.1 angstrom crystal structure double reveals side-by-side, four-helix bundles with highly polarized surface charge distribution. Each bundle an Nepsilon-acetyllysine...