A5076938568- Virology and Viral Diseases
- SARS-CoV-2 and COVID-19 Research
- Vaccine Coverage and Hesitancy
- vaccines and immunoinformatics approaches
- Glioma Diagnosis and Treatment
- Pneumonia and Respiratory Infections
- Respiratory viral infections research
- Bacterial Infections and Vaccines
- COVID-19 Clinical Research Studies
- Drug Transport and Resistance Mechanisms
- Immunotherapy and Immune Responses
- Acute Myeloid Leukemia Research
- T-cell and B-cell Immunology
- Retinoids in leukemia and cellular processes
- Viral gastroenteritis research and epidemiology
- Animal Disease Management and Epidemiology
- 3D Printing in Biomedical Research
- Neuroblastoma Research and Treatments
- Acute Lymphoblastic Leukemia research
- Glycosylation and Glycoproteins Research
- Influenza Virus Research Studies
- COVID-19 Impact on Reproduction
- Proteoglycans and glycosaminoglycans research
- Cell Adhesion Molecules Research
- Hepatitis Viruses Studies and Epidemiology
National Institute for Public Health and the Environment
2016-2025
Eindhoven Cancer Registry
2020
National Centre for Infectious Diseases
2013-2015
TuBerculosis Vaccine Initiative
2008-2009
Vrije Universiteit Amsterdam
2003-2006
Leiden University Medical Center
2005
Amsterdam UMC Location Vrije Universiteit Amsterdam
2003
University of Amsterdam
1995-2001
Amsterdam UMC Location University of Amsterdam
1995-2001
Graduate School Neurosciences Amsterdam Rotterdam
1996
Background We aimed to detect SARS-CoV-2 serum antibodies in the general population of Netherlands and identify risk factors for seropositivity amidst first COVID-19 epidemic wave. Methods Participants (n=3207, aged 2–90 years), enrolled from a previously established nationwide serosurveillance study, provided self-collected fingerstick blood sample completed questionnaire (median inclusion date 3 April 2020). IgG targeted against spike S1-protein were quantified using validated...
After the COVID-19 pandemic, significant increases in measles cases were observed globally. Community-wide vaccination remains most effective strategy for preventing measles. However, it is crucial to understand whether prevalent genotypes, when circulating populations with suboptimal coverage, may undergo adaptive mutations that allow them escape vaccine-induced immunity. In this study, a bioinformatics-guided approach was used predict universal helper T-cell epitopes specific vaccine virus...
An improved detergent-free process has been developed to produce vaccine based on native outer membrane vesicles (NOMV) against Neisseria meningitidis serogroup B. Performance was evaluated with the NonaMen concept, which provides broad coverage nine distinct PorA antigens. Scalable aseptic equipment implemented, replacing undesirable steps like ultracentrifugation, inactivation phenol, and use of preservatives. The resulting is more consistent gives a higher yield than published reference...
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has led to considerable morbidity/mortality worldwide, but most infections, especially among children, have a mild course. However, it remains largely unknown whether infected children develop cellular immune memory.To determine memory T cell response is being developed, we performed longitudinal assessment of the SARS-CoV-2-specific by IFN-γ ELISPOT and activation marker analyses peripheral blood samples from unvaccinated adults...
Waning of vaccine-induced immunity is considered to play a central role in the reemergence mumps among vaccinated young adults. The aim present study was investigate antibody responses and safety third dose measles-mumps-rubella vaccine (MMR-3) 150 Antibody levels were related surrogate protection based on preoutbreak serum 31 persons with 715 without serological evidence mumps.Mumps virus-specific immunoglobulin G (IgG) virus-neutralizing (based focus-reduction neutralizing test) against...
Influenza-like illness (ILI) can be caused by a range of respiratory viruses. The present study investigates the contribution influenza and other viruses, occurrence viral co-infections, persistence viruses after ILI onset in older adults. During season 2014–2015, 2366 generally healthy community-dwelling adults (≥60 years) were enrolled study. Viruses identified multiplex ligation–dependent probe-amplification assay naso- oropharyngeal swabs taken during acute phase, 2 8 weeks later....
Omicron BA.1 variant can readily infect people with vaccine-induced or naturally acquired SARS-CoV-2 immunity facilitated by escape from neutralizing antibodies. In contrast, T-cell reactivity against the seems relatively well preserved. Here, we studied preexisting T cells elicited either vaccination mRNA-based BNT162b2 vaccine natural infection ancestral for their cross-reactive potential to 20 selected CD4+ epitopes of spike-protein-harboring mutations. Although overall memory responses...
The first meningococcal serogroup C (MenC) conjugate vaccine was licensed in 1999 and introduced the United Kingdom. Countries that have implemented MenC since then their national immunisation programmes use different schedules. Nevertheless, all involved countries seem to experience substantial declines incidence of disease. Since 2001, has been Netherlands by offering a single dose children aged 14 months. Prior introduction into programme, catch-up vaccination campaign initiated which...
Mumps outbreaks and breakthrough infections of measles rubella have raised concerns about waning vaccine-induced immunity after two doses measles-mumps-rubella (MMR) vaccination. In the present follow-up study, serum IgG antibodies against mumps, rubella, as well functional neutralizing both mumps vaccine strain outbreak strains were measured longitudinally in young adults that received a third MMR (MMR3) dose. The mumps-specific virus antibody levels at 3 years vaccination still elevated...
Abstract A variety of antigens that display a highly tissue-specific expression pattern have recently found to be also expressed in medullary thymic epithelial cells (mTEC). This unique feature mTEC plays an important role preventing hazardous autoimmune responses through tolerization T-cell subsets directed against autoantigens but could limit the possibility exploiting tumor-associated for immune-mediated targeting cancers. Our present study shows carcinoembryonic antigen (CEA)...
As reported in a recent issue of Eurosurveillance, mumps outbreak is ongoing the Netherlands despite high vaccination coverage 90-95% [1]. The cases are restricted to geographic regions with percentage residents who members religious community that rejects vaccination. Consequently, two thirds patients were not vaccinated. However, also vaccinated individuals these affected Since 1987, measles-mumps-rubella (MMR) combination vaccine produced by Vaccine Institute (NVI) part Dutch national...
Tick-borne encephalitis and West Nile fever are endemic flavivirus diseases in Europe. Climate change, virus evolution, social factors may increase the risk of these infections lead to emergence other flaviviruses Europe that (sub)tropical regions world. Control spread is very difficult considering cycling between arthropod vectors animal reservoir hosts. The increasing threat emphasizes necessity a sustainable vector surveillance system, an active health system adequate human for early...
The respiratory syncytial virus (RSV) is the major cause of lower tract illness (LRI) in infants worldwide. Also persons with heart/lung disease or an immunodeficiency disorder, and elderly are at increased risk for severe LRI upon RSV infection. Although there present no licensed vaccine available, it a priority target several developers. For implementation future vaccination within national immunization schemes, various strategies can be considered even without availability extended...
SARS-CoV-2 Omicron (B.1.1.529) lineages rapidly became dominant in various countries reflecting its enhanced transmissibility and ability to escape neutralizing antibodies. Although T cells induced by ancestral SARS-CoV-2-based vaccines also recognize variants, we showed our previous study that there was a marked loss of cell cross-reactivity spike epitopes harboring BA.1 mutations. The emerging BA.4/BA.5 subvariants carry other mutations than the variant. present aims investigate impact on...
Last decades, the number of routine childhood vaccinations has increased considerably, which consequently led to multiple vaccine injections per consultation. Implementation additional vaccines will probably lead more than 2 consult, might be a barrier for parents vaccinate their child. A decrease in vaccination coverage, however, increases risk disease outbreaks. Less stressful alternative methods delivery an acceptance by parents. The present questionnaire study was set up explore maximum...
Breakthrough infections of measles and mumps have raised concerns about the duration vaccine-induced immunity, which might be improved by a third dose measles-mumps-rubella vaccine (MMR3). Here we compared (IgG) antibody levels against measles, mumps, rubella in blood samples 9-year-old children young adults (18-25 years) following MMR2 MMR3, respectively. We found that, addition to boosting for all 3 components, MMR3 resulted lower decay rates than MMR2; declines were most prominent...
Bone marrow and peripheral blood samples from 362 patients with acute lymphoblastic leukaemia (ALL) proliferating cell 90 myeloid (AML) were analysed for S-phase fractions, Ki67 antigen, nuclear antigen expression. The fractions correlated in vitro drug resistance to 15 different anticancer agents. Leukaemia cells isolated bone had higher than (in initial ALL, median values resp. 6.9 2.7%, AML 5.3 1.3%; both P<0.01). Relapse ALL derived showed increased (median 9.9%) compared 6.9%; obtained...