A5081958362- Adipose Tissue and Metabolism
- Muscle metabolism and nutrition
- Respiratory viral infections research
- Metabolism, Diabetes, and Cancer
- Diet and metabolism studies
- Muscle Physiology and Disorders
- Circular RNAs in diseases
- Sports Performance and Training
- MicroRNA in disease regulation
- Exercise and Physiological Responses
- Adenosine and Purinergic Signaling
- Urinary Tract Infections Management
- Phytoestrogen effects and research
- Lipid metabolism and biosynthesis
- Diabetes Management and Research
- Hormonal Regulation and Hypertension
- Cancer-related molecular mechanisms research
- Pancreatic function and diabetes
- Natural product bioactivities and synthesis
- Peroxisome Proliferator-Activated Receptors
- Sexual Differentiation and Disorders
- Metabolism and Genetic Disorders
- Parkinson's Disease Mechanisms and Treatments
- Ginseng Biological Effects and Applications
- Spinal Cord Injury Research
St. Antonius Ziekenhuis
2024-2025
Karolinska Institutet
2012-2015
Institute of Molecular Medicine
2013
Maastricht University Medical Centre
2013
Maastricht University
2006-2008
Erasmus MC - Sophia Children’s Hospital
1972
The identification of microRNAs (miRNAs) has established new mechanisms that control skeletal muscle adaptation to exercise. present study investigated the mRNA regulation components miRNA biogenesis pathway (Drosha, Dicer and Exportin-5), enriched miRNAs, (miR-1, -133a, -133b -206), several miRNAs dysregulated in myopathies (miR-9, -23, -29, -31 -181). Measurements were made biopsies from nine healthy untrained males at rest, 3 h following an acute bout moderate-intensity endurance cycling...
Abstract Background While influenza virus and severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) are recognised as a cause of illness mortality, clinical interest for syncytial (RSV), rhinovirus human metapneumovirus (hMPV) infections is still limited. Methods We conducted retrospective database study comparing baseline characteristics 30‐day mortality in large cohort adult patients admitted an overnight stay or longer with (A/B), rhinovirus, hMPV, RSV SARS‐CoV‐2 infection. For...
Abstract Background Enteroviruses (EV) cause yearly outbreaks with severe infections, particularly in young children. This study investigates EV circulation, age-distribution, and clinical presentations Europe from 2018-2023. Methods Aggregated data were requested ECDC National Focal Points for Surveillance European Non-Polio Enterovirus Network. Data included detection month, specimen type, age-group, presentation the ten most commonly reported types per year. Findings Twenty-eight...
The 5′-AMP-activated protein kinase (AMPK) pathway is intact in type 2 diabetic patients and seen as a target for diabetes treatment. In this study, we aimed to assess the impact of AMPK activator 5-aminoimidazole-4-carboxamide riboside (AICAR) on both glucose fatty acid metabolism vivo patients. Stable isotope methodology blood muscle biopsy sampling were applied kinetics following continuous i.v. infusion AICAR (0.75 mg kg−1 min−1) and/or NaCl (0.9%) ten male (age 64 ± years; BMI 28 1...
Using contemporary stable-isotope methodology and fluorescence microscopy, we assessed the impact of carbohydrate supplementation on whole-body fiber-type-specific intramyocellular triacylglycerol (IMTG) glycogen use during prolonged endurance exercise. Ten endurance-trained male subjects were studied twice 3 h cycling at 63 ± 4% maximal O2 uptake with either glucose ingestion (CHO trial; 0.7 g CHO kg−1 h−1) or without (CON placebo water only). Continuous infusions [U-13C] palmitate...
ABSTRACT Enzyme preparations were isolated from post-mortem adrenal tissue of a patient with lipoid hyperplasia and normal human- bovine tissue. Cholesterol-cleaving properties studied in incubation experiments the addition appropriate substrates. activity enzyme was extremely low as compared controls. There no difference 20α-hydroxycholesterol-cleaving activity. is thus evidence for deficient 20α-cholesterol-hydroxylase this hyperplasia.
Impaired regulation of lipolysis and accumulation lipid intermediates may contribute to obesity-related insulin resistance type 2 diabetes mellitus. We investigated insulin-mediated suppression in abdominal subcutaneous adipose tissue (AT) skeletal muscle (SM) obese men with normal glucose tolerance (NGT) diabetic men.Eleven NGT nine long-term diagnosed (7 ± 1 years), matched for age (58 vs 62 BMI (31.4 0.6 30.5 kg/m(2)) [Formula: see text] (28.9 1.5 29.5 2.4 ml kg(-1) min(-1)) participated...
Intramyocellular triacylglycerol (IMTG) has been suggested to represent an important substrate source during exercise. In the present study, IMTG utilization exercise is assessed through use of various methodologies. addition, we identified differences in intramyocellular lipids deposited immediate subsarcolemmal (SS) area and those stored more central region fiber. Contemporary stable isotope technology was applied combination with muscle tissue sampling before immediately after 3 h...
Abstract Objective: Hospital-onset bacteremia and fungemia (HOB) has been suggested as a suitable automatable surveillance target to include in programs, however differences definitions across studies limit interpretation large-scale implementation. We aimed apply an automated system for HOB multiple hospitals using consensus definition, describe rates. Design setting: Retrospective cohort study four Dutch hospitals: 1 tertiary hospital 3 secondary hospitals. Patients: All patients admitted...
Na(+)-K(+)-ATPase is an integral membrane protein crucial for the maintenance of ion homeostasis and skeletal muscle contractibility. Skeletal content displays remarkable plasticity in response to long-term increase physiological demand, such as exercise training. However, adaptations function a suddenly decreased and/or habitually low level physical activity, especially after spinal cord injury (SCI), are incompletely known. We tested hypothesis that associated regulatory proteins from FXYD...
Abstract Aim: Membrane fatty acid transporters can modulate the balance between uptake and subsequent storage and/or oxidation in muscle tissue. As such, skeletal transporter protein expression could play an important role etiology of insulin resistance type 2 diabetes. Methods: In present study, translocase (FAT/CD36), plasma membrane‐bound acid‐binding (FABPpm) transport 1 (FATP1) mRNA were assessed tissue obtained from 10 sedentary, overweight diabetes patients (60 ± years),...
Skeletal muscle plays an important role in the regulation of energy homeostasis; therefore, ability skeletal to adapt and alter metabolic gene expression response changes physiological demands is critical for balance. Individuals with cervical spinal cord lesions are characterized by tetraplegia, impaired thermoregulation, altered morphology. We patterns, as well protein content, these individuals assess impact injury on determinants metabolism. Our results demonstrate that mRNA levels genes...
The effects of long-term physical inactivity on the expression microRNAs involved in regulation skeletal muscle mass humans are largely unknown. MicroRNAs short, noncoding RNAs that fine-tune target through mRNA degradation or by inhibiting protein translation. Intronic to slow, type I, fiber genes MYH7 and MYH7b, microRNA-208b microRNA-499-5p thought important for growth, such as myostatin. Spinal cord injured characterized both atrophy transformation toward fast-twitch, II fibers. We...
AMP-activated protein kinase (AMPK) is a pivotal regulator of energy homeostasis. Although downstream targets AMPK are widely characterized, the physiological factors governing isoform expression this largely unknown. Nerve/contractile activity has major impact on metabolic phenotype skeletal muscle, therefore likely to influence expression. Spinal cord injury represents an extreme form physical inactivity, with concomitant changes in muscle metabolism. We assessed longstanding and recent...
The purpose of this study was to compare substrate source use in older, long-term exercising, endurance-trained males with sedentary controls.[U-C]palmitate and [6,6-H2]glucose tracers were applied assess plasma free fatty acid (FFA) glucose oxidation rates, estimate muscle- and/or lipoprotein-derived triacylglycerol (TG) muscle glycogen use. Subjects 10 controls (age 57 +/- 1 60 2 yr, respectively). Muscle biopsy samples collected before after exercise fiber type-specific intramyocellular...
In animal models, administration of the adenosine analog AICA-riboside has shown beneficial effects on ischemia-reperfusion injury and glucose homeostasis. The vascular and/or metabolic in humans remain to be established. was infused intra-arterially four different dosages up 8 mg x min(-1) dl(-1) 24 healthy subjects. Forearm blood flow (FBF) uptake plasma glucose, free fatty acid, concentrations were assessed. We also combined infusion (2 dl(-1)) with intra-arterial receptor antagonist...
The effect of carbohydrate supplementation on muscle glycogen and intramyocellular triacylglycerol (IMTG) utilization during prolonged cycling is equivocal, as most studies have failed to observe sparing or altered IMTG use. PURPOSE: Using contemporary stable isotope methodology fluorescence microscopy, we assessed the impact whole-body fiber-type specific use exercise. METHODS: Ten male subjects were studied twice 3 h at 63±1% maximal O2 uptake with continuous glucose ingestion (CHO trial;...