A5021269916- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Diet and metabolism studies
- Lipid metabolism and biosynthesis
- Cardiovascular Disease and Adiposity
- Gut microbiota and health
- Muscle metabolism and nutrition
- Pancreatic function and diabetes
- Vitamin D Research Studies
- Liver Disease Diagnosis and Treatment
- Lipid metabolism and disorders
- Hormonal Regulation and Hypertension
- Pharmacological Effects and Assays
- Autophagy in Disease and Therapy
- Vitamin C and Antioxidants Research
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Tea Polyphenols and Effects
- Sirtuins and Resveratrol in Medicine
- Metabolism, Diabetes, and Cancer
- Regulation of Appetite and Obesity
- Immune Cell Function and Interaction
- Gastrointestinal motility and disorders
- Dietary Effects on Health
- Nutritional Studies and Diet
- Metabolomics and Mass Spectrometry Studies
Maastricht University
2013-2024
Maastricht University Medical Centre
2014-2024
TiFN
2017-2018
University Medical Center
2016
Karolinska Institutet
2009-2011
Karolinska University Hospital
2009-2011
Inserm
2011
Sorbonne Université
2011
Joanneum Research
2011
Background— Adipose tissue (AT) dysfunction in obesity contributes to chronic, low-grade inflammation that predisposes type 2 diabetes mellitus and cardiovascular disease. Recent vitro studies suggest AT hypoxia may induce inflammation. We hypothesized adipose blood flow (ATBF) regulates oxygen partial pressure (AT P o ), thereby affecting insulin sensitivity. Methods Results— developed an optochemical measurement system for continuous monitoring of using microdialysis. The effect...
Abstract Short-chain fatty acids (SCFA), formed by microbial fermentation, are believed to be involved in the aetiology of obesity and diabetes. This study investigated effects colonic administration physiologically relevant SCFA mixtures on human substrate energy metabolism. In this randomized, double-blind, crossover study, twelve normoglycaemic men (BMI 25–35 kg/m 2 ) underwent four investigational days, during which (200 mmol/L) high either acetate (HA), propionate (HP), butyrate (HB) or...
Abstract Microbial-derived short-chain fatty acids (SCFA) acetate, propionate and butyrate may provide a link between gut microbiota whole-body insulin sensitivity (IS). In this cross-sectional study (160 participants, 64% male, BMI: 19.2–41.0 kg/m 2 , normal or impaired glucose metabolism), associations SCFA (faecal fasting circulating) circulating metabolites, substrate oxidation IS were investigated. subgroup (n = 93), was determined using hyperinsulinemic-euglycemic clamp. Data analyzed...
Abstract Obesity is associated with a disturbed adipose tissue (AT) function characterized by adipocyte hypertrophy, an impaired lipolysis and pro-inflammatory phenotype, which contributes to insulin resistance (IR). We investigated whether AT phenotype in different depots of obese individuals without type 2 diabetes mellitus (T2DM) whole-body IR. Subcutaneous (SC) visceral (V) biopsies from 18 lean, 17 8 T2DM men were collected. was ex vivo measurement basal stimulated (mature adipocytes),...
Obesity is associated with increased triacylglycerol (TAG) storage in adipose tissue and insulin resistance. The mobilization of stored TAG mediated by hormone-sensitive lipase (HSL) the recently discovered triglyceride (ATGL). aim present study was to examine whether ATGL HSL mRNA protein expression are altered insulin-resistant conditions. In addition, we investigated a possible impaired could be reversed period weight reduction.Adipose biopsies were taken from obese subjects (n = 44) wide...
Lipolysis is the catabolic pathway by which triglycerides are hydrolyzed into fatty acids. Adipose triglyceride lipase (ATGL) and hormone-sensitive (HSL) have capacity to hydrolyze in vitro first ester bond of triglycerides, but their respective contributions whole cell lipolysis human adipocytes unclear. Here, we investigated roles HSL, ATGL, its coactivator CGI-58 basal forskolin-stimulated a white adipocyte model, hMADS cells. The express various components acid metabolism show lipolytic...
Gut-derived short-chain fatty acids (SCFA), formed by microbial fermentation of dietary fibers, are believed to be involved in the etiology obesity and diabetes. Previous data from our group showed that colonic infusions physiologically relevant SCFA mixtures attenuated whole-body lipolysis overweight men. To further study potential mechanisms antilipolytic properties SCFA, we aimed investigate vitro effects incubations on intracellular signaling using a human white adipocyte model,...
Hormone-sensitive lipase (HSL) and adipose triglyceride (ATGL) regulate adipocyte lipolysis in rodents. The purpose of this study was to compare the roles these lipases for human adipocytes. Subcutaneous tissue investigated. HSL ATGL protein expression were related isolated mature fat cells. or knocked down by RNA interference (RNAi) selectively inhibited, effects on studied differentiated preadipocytes adipocytes derived from mesenchymal stem cells (hMSC). Subjects all women. There 12 lean...
Abstract Immune cell accumulation in adipose tissue (AT) is associated with the development of AT inflammation, resulting metabolic dysfunction. Circulating immune patterns may reflect expanding AT. However, data linking human leukocytes blood and lacking. We investigated whether populations are their counterparts subcutaneous (scAT) or visceral (vAT). Flow cytometry was performed on blood, scAT vAT from 16 lean 29 obese men. natural killer (NK)-cells, classical monocytes nonclassical were...
Impaired regulation of lipolysis and accumulation lipid intermediates may contribute to obesity-related insulin resistance type 2 diabetes mellitus. We investigated insulin-mediated suppression in abdominal subcutaneous adipose tissue (AT) skeletal muscle (SM) obese men with normal glucose tolerance (NGT) diabetic men.Eleven NGT nine long-term diagnosed (7 ± 1 years), matched for age (58 vs 62 BMI (31.4 0.6 30.5 kg/m(2)) [Formula: see text] (28.9 1.5 29.5 2.4 ml kg(-1) min(-1)) participated...
Acute intake of the wheat bran extract Arabinoxylan-Oligosaccharide (AXOS) modulates gut microbiota, improves stool characteristics and postprandial glycemia in healthy humans. Yet, little is known on how long-term AXOS influences gastrointestinal (GI) functioning, metabolic health. In this randomized, placebo-controlled, double-blind study, we evaluated effects GI function health adults with slow transit without constipation. Forty-eight normoglycemic were included whole-gut time (WGTT) >35...
Obesity-related insulin resistance (IR) may develop in multiple organs, representing various etiologies for cardiometabolic diseases. We identified abdominal subcutaneous adipose tissue (ScAT) transcriptome profiles liver or muscle IR by means of RNA sequencing overweight obese participants the Diet, Obesity, and Genes (DiOGenes) (NCT00390637, ClinicalTrials.gov) cohort (n = 368). Tissue-specific phenotypes were derived from a 5-point oral glucose tolerance test. Hepatic characterized...
Obesity is characterised by increased triacylglycerol storage in adipose tissue. There vitro evidence for a blunted beta-adrenergically mediated lipolytic response abdominal subcutaneous tissue (SAT) of obese individuals and this at the whole-body level vivo. We hypothesised that effect on lipolysis SAT also impaired vivo humans.We investigated glycerol metabolism during 3 h 6 [2H5]glycerol infusions. Arterio-venous concentration differences were measured 13 lean ten men after an overnight...
The Liver X receptor (LXR) is an important regulator of carbohydrate and lipid metabolism in humans mice. We have recently shown that activation LXR regulates cellular fuel utilization adipocytes. In contrast, the role human adipocyte lipolysis, major function white fat cells, not clear. present study, we stimulated vitro differentiated murine adipocytes with agonist GW3965 observed increase basal lipolysis. Microarray analysis mRNA following revealed altered gene expression several...
Rodent studies have indicated that physical exercise may improve adipose tissue function. We investigated the effects of a 12-wk supervised, progressive training program on adipocyte morphology and abdominal subcutaneous function in metabolically well-phenotyped subjects with obesity. Men obesity ( n = 21) participated progressive, combined consisting aerobic (30 min at 70% maximal power output 2 times/wk) resistance (3 × 10 repetitions 60% 1 repeated maximum time/wk), adjustment intensity...