A5050834302- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Immune Cell Function and Interaction
- Lipid metabolism and biosynthesis
- Regulation of Appetite and Obesity
- Cardiovascular Disease and Adiposity
- Gut microbiota and health
- Metabolism, Diabetes, and Cancer
- Diet and metabolism studies
- Immune cells in cancer
- Diabetes and associated disorders
- Fatty Acid Research and Health
- Atherosclerosis and Cardiovascular Diseases
- Helicobacter pylori-related gastroenterology studies
- Diet, Metabolism, and Disease
- Cholesterol and Lipid Metabolism
- Pancreatic function and diabetes
- Pharmacological Effects and Assays
- Mitochondrial Function and Pathology
- GDF15 and Related Biomarkers
- Pharmacology and Obesity Treatment
- Nutrition, Genetics, and Disease
- Nutritional Studies and Diet
- Lipoproteins and Cardiovascular Health
- Vitamin D Research Studies
Leiden University Medical Center
2013-2022
Maastricht University
1999-2013
Loyola University Medical Center
2013
Leiden University
2011
Karolinska Institutet
1998-2010
Karolinska University Hospital
1997-2010
Bristol-Myers Squibb (Germany)
2007
Else Kröner-Fresenius-Stiftung
2004-2005
Fresenius (Germany)
2004
Technical University of Munich
2003-2004
The mobilization of fat stored in adipose tissue is mediated by hormone-sensitive lipase (HSL) and the recently characterized triglyceride (ATGL), yet their relative importance lipolysis unknown. We show that a novel potent inhibitor HSL does not inhibit other lipases. compound counteracted catecholamine-stimulated mouse adipocytes had no effect on residual hydrolysis HSL-null mice. In human adipocytes, catecholamine- natriuretic peptide–induced were completely blunted inhibitor. When cells...
Chronic low-grade inflammation associated with obesity contributes to insulin resistance and type 2 diabetes. Helminth parasites are the strongest natural inducers of immune responses, short-lived infection rodent nematodes was reported improve glucose tolerance in obese mice. Here, we investigated effects chronic (12 weeks) Schistosoma mansoni, a helminth that infects millions humans worldwide, on whole-body metabolic homeostasis white adipose tissue (WAT) cell composition high-fat...
Loss of fat mass is a key feature cancer cachexia and has been attributed to increased adipocyte lipolysis. The mechanism behind this alteration unknown was presently investigated. We studied mature s.c. cells differentiated preadipocytes from 26 patients with without cachexia. Hormone-induced lipolysis expression lipolysis-regulating genes were determined together body composition in vivo lipolytic activity (fasting plasma glycerol or fatty acids related fat). Body reduced by 40% 2-fold (P...
Tumor necrosis factor-α (TNF-α) is a pleiotropic cytokine with proposed role in obesity-related insulin resistance. This could be mediated by increased lipolysis adipose tissue resulting elevated free fatty acid levels. The early intracellular signals entailed TNF-α-mediated are unknown but may involve members of the mitogen-activated protein kinase (MAPK) family. We investigated possible contribution MAPK TNF-α-induced human preadipocytes. TNF-α activated three mammalian MAPK, p44/42, JNK,...
Site differences in adipose tissue function may have implications for insulin-resistant conditions. In mature tissue, subcutaneous adipocytes higher leptin secretion, similar tumor necrosis factor (TNF)-α and lower catecholamine-stimulated lipolysis as compared with omental adipocytes. this study, TNF-α secretion were between human preadipocytes. After 16 days of incubation a minimal differentiation medium, mRNA found to be two eight times than preadipocytes (P < 0.05). On the other...
Tumor necrosis factor-α (TNF-α) promotes lipolysis in mammal adipocytes via the mitogen-activated protein kinase (MAPK) family, resulting reduced expression/function of perilipin (PLIN). The role another pivotal intracellular messenger activated by TNF-α, nuclear factor-κB (NF-κB), has not been recognized. We explored NF-κB TNF-α-induced human fat cells. Primary cultures were incubated presence a cell-permeable peptide that inhibits signaling (WP). Incubation with WP, but biologically...
Abstract Objective: Adipose angiotensinogen has been suggested as a stimulator of adipose tissue growth and development. Therefore, the association subcutaneous gene expression with human obesity was studied. Research Methods Procedures: The study group consisted 17 men, undergoing either gastric banding for or elective laparoscopic cholecystectomy (7 obese, 10 non‐obese men; body mass index 22 to 51 kg/m 2 ; age 26 68 years). Subcutaneous mRNA 18S ribosomal RNA (reference gene) levels were...
The effects of acylation-stimulating protein (ASP) and insulin on free fatty acid (FFA) release from isolated human fat cells the signal transduction pathways to induce these were studied. ASP inhibited basal norepinephrine-induced FFA by stimulating fractional re-esterification (both same extent) inhibiting produced during lipolysis (ASP a lesser extent than insulin). Protein kinase C inhibition influenced none or insulin. Phosphatidylinositol 3-kinase counteracted but not ASP....
Studies have shown evidence of production nitric oxide (NO) in adipose tissue, as well inhibition lipolysis by NO. We analyzed synthase (NOS) expression subcutaneous tissue from 13 nonobese and 18 obese male subjects. Using a competitive reverse transcription polymerase chain reaction method, endothelial (eNOS) inducible (iNOS), but not neuronal (nNOS), mRNA was detected isolated fat cells pieces tissue. Tissue levels for eNOS were 3,814 ± 825 5,956 476 amol/mg RNA (P = 0.043), iNOS 306 38...
Hormone-sensitive lipase (HSL) and adipose triglyceride (ATGL) regulate adipocyte lipolysis in rodents. The purpose of this study was to compare the roles these lipases for human adipocytes. Subcutaneous tissue investigated. HSL ATGL protein expression were related isolated mature fat cells. or knocked down by RNA interference (RNAi) selectively inhibited, effects on studied differentiated preadipocytes adipocytes derived from mesenchymal stem cells (hMSC). Subjects all women. There 12 lean...
Several proinflammatory cytokines including IL-6 and IL-8 are produced by human adipocytes, but it is still unclear how this process regulated. Angiotensin (Ang) II, which also might play a role as regulator. In the present study, we investigated effect of Ang II on production in vitro differentiated adipocytes.Isolation preadipocytes differentiation these cells into Real-time quantitative reverse-transcriptase polymerase chain reaction, Western-blot, enzyme-linked immunosorbent assay,...
Obesity and type 2 diabetes mellitus (T2DM) have been associated with increased levels of circulating branched-chain amino acids (BCAAs) that may be involved in the pathogenesis insulin resistance. However, weight loss has not consistently reduction BCAA levels.We included 30 obese normal glucose-tolerant (NGT) subjects, 32 subjects T2DM, 12 lean female subjects. Obese underwent either a restrictive procedure (gastric banding [GB], very low-calorie diet [VLCD]), or restrictive/bypass...
Scope Mannan oligosaccharides (MOS) have proven effective at improving growth performance, while also reducing hyperlipidemia and inflammation. As atherosclerosis is accelerated both by inflammation, we aim to determine the effect of dietary MOS on development in hyperlipidemic ApoE*3‐Leiden.CETP ( E3L.CETP ) mice, a well‐established model for human‐like lipoprotein metabolism. Methods results Female mice were fed high‐cholesterol diet, with or without 1% 14 weeks. substantially decreased...
White adipose tissue (WAT) consists of various depots with different adipocyte functionality and immune cell composition. Knowledge WAT-depot-specific differences in expandability influx during the development obesity is limited, therefore we aimed to characterise WAT mice. Gonadal (gWAT), subcutaneous (sWAT) mesenteric (mWAT) were isolated from male C57Bl/6J mice body weights (approximately 25–60 g) analysed. Linear non-linear regression models used describe extent depot composition as a...
Akkermansia muciniphila (A. muciniphila) is an intestinal commensal with anti-inflammatory properties both in the intestine and other organs. The aim to investigate effects of oral administration A. on lipid metabolism, immunity, cuff-induced neointima formation hyperlipidemic APOE*3-Leiden (E3L).CETP mice.Hyperlipidemic male E3L.CETP mice are daily treated 2 × 108 CFU by gavage for 4 weeks determined plasma levels, immune parameters, composition. lowers body weight total cholesterol...
The composition of microbial communities is commonly determined by sequence analyses one the variable (V) regions in bacterial 16S rRNA gene. We aimed to assess whether sequencing full-length versus V4 region gene affected results and interpretation an experiment. To test this, mice were fed a diet without with prebiotic inulin from cecum samples, two primary data sets generated: (1) set generated PacBio platform; (2) Illumina MiSeq platform. A third derived was silico extracting set....
Hormone-sensitive lipase (HSL) catalyzes the rate-limiting step in adipocyte lipolysis. The activity of HSL is thought to be primarily regulated by reversible phosphorylation. However, regulation pretranslational mechanisms has been poorly studied. present studies were undertaken explore relationship between levels protein and mRNA expressions lipolytic capacity. study was performed human abdominal subcutaneous adipocytes with identical sizes but having either a high (HL) or low (LL)...
Visceral obesity increases risk of insulin resistance and type 2 diabetes. This may partly be due to a region-specific insulin's antilipolytic effect in visceral adipocytes. We investigated whether adipose tissue releases the vascular peptide endothelin-1 (ET-1) ET-1 could account for regional differences lipolysis.One group consisted eleven obese nonobese subjects whom levels were compared between abdominal subcutaneous arterialized blood samples. A second included undergoing anti-obesity...