A5012437757- Mosquito-borne diseases and control
- T-cell and Retrovirus Studies
- Vector-Borne Animal Diseases
- Animal Disease Management and Epidemiology
- Viral Infections and Vectors
- Neuroinflammation and Neurodegeneration Mechanisms
- interferon and immune responses
- Neonatal and fetal brain pathology
- RNA regulation and disease
- Cytomegalovirus and herpesvirus research
- Influenza Virus Research Studies
- MicroRNA in disease regulation
- Viral Infections and Immunology Research
- Cardiovascular Effects of Exercise
- HIV Research and Treatment
- Cancer-related molecular mechanisms research
- Virology and Viral Diseases
- Immune Cell Function and Interaction
- Muscle and Compartmental Disorders
- Evolution and Paleontology Studies
- Autoimmune and Inflammatory Disorders Research
- Innovation and Socioeconomic Development
- Insect-Plant Interactions and Control
- Antimicrobial Peptides and Activities
- Retinal Development and Disorders
Institut Pasteur
2015-2024
Centre National de la Recherche Scientifique
2013-2024
Université Paris Cité
2020-2024
Centre de Gestion Scientifique
2016-2020
Institut Gustave Roussy
2017
Université Paris-Saclay
2017
Inserm
2008-2012
Hôpital Paul-Brousse
2008
Surveillance of the antiviral susceptibility influenza viruses in Europe revealed emergence A(H1N1) naturally resistant to anti-neuraminidase inhibitor oseltamivir (Tamiflu) [1]. Currently, are most prevalent (25%) but less Americas (16%) or Western Pacific region (4%) [2]. In Europe, prevalence varies between countries, with highest levels Norway (66.5%) and France (46.6%) [3]. These frequencies sharp contrast those observed for H1N1 during previous seasons (0 <1%) [4]–[8].
HIV controllers are rare individuals who spontaneously control replication in the absence of antiretroviral treatment. Emerging evidence indicates that is mediated through very active cellular immune responses, though how such responses can persist over time without exhaustion not yet understood. To investigate nature memory CD4+ T cells responsible for long-term anti-HIV we characterized growth kinetics, Vβ repertoire, and avidity antigen patient-derived primary cell lines. Specific lines...
HIV controllers are rare individuals who spontaneously control replication in the absence of antiretroviral therapy. To identify parameters CD4 response that may contribute to viral rather than merely reflect a persistently low viremia, we compared T helper profiles two groups patients with more 10 years suppression: from Agence Nationale de Recherche sur le SIDA et les Hépatites Virales (ANRS) CO18 cohort (n = 26) and efficiently treated 16). Cells specific for immunodominant Gag...
Abstract Extracellular vesicles (EVs) can participate in intercellular communication and pathogenesis. EVs contain many cargos, including proteins, the composition of differs between cell-types activation levels. Thus, plasma be used as a biomarker systemic response to infection and/or disease progression. In this study, we aimed at describing alterations protein content upon with human T-lymphotropic retrovirus type 1 (HTLV-1). HTLV-1 is etiological agent lymphoproliferative (ATL) series...
Besides the classical respiratory and systemic symptoms, unusual complications of influenza A infection in humans involve skeletal muscles. Numerous cases acute myopathy and/or rhabdomyolysis have been reported, particularly following outbreak pandemic A(H1N1) 2009. The pathogenesis these influenza-associated myopathies (IAM) remains unkown, although direct muscle cells is suspected. Here, we studied susceptibility cultured human primary to a 2009 2008 seasonal isolate. Using from different...
ABSTRACT Human T-lymphotropic virus type 1 (HTLV-1) is the etiological agent of a slowly progressive neurodegenerative disease, HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). This disease develops upon infiltration HTLV-1-infected lymphocytes into central nervous system, mostly thoracic spinal cord. The system normally protected by physiological structure called blood-brain barrier (BBB), which consists primarily continuous endothelium with tight junctions. In this...
Abstract The human T-lymphotropic virus type 1 (HTLV-1) is efficiently transmitted through cellular contacts. While the molecular mechanisms of viral cell-to-cell propagation have been extensively studied in vitro , those facilitating encounter between infected and target cells remain unknown. In this study, we demonstrate that HTLV-1-infected CD4 T secrete a potent chemoattractant, leukotriene B4 (LTB4). LTB4 secretion dependent on Tax-induced transactivation pla2g4c gene, which encodes...
Muscle cells are potential targets of many arboviruses, such as Ross River, Dengue, Sindbis, and chikungunya viruses, that may be involved in the physiopathological course infection. During recent outbreak Zika virus (ZIKV), myalgia was one most frequently reported symptoms. We investigated susceptibility human muscle to ZIKV Using an vitro model primary myoblasts can differentiated into myotubes, we found productively infected by ZIKV. In contrast, myotubes were shown resistant infection,...
Abstract Background Several forms of progressive retinal atrophy (PRA) segregate in more than 100 breeds dog with each PRA segregating one or a few breeds. This breed specificity may be accounted for by founder effects and genetic drift, which have reduced the heterogeneity breed, thereby facilitating identification causal mutations. We report here new form Border Collie breed. The clinical signs, including loss night vision day vision, resulting complete blindness, occur at age three to...
Abstract First isolated in 1953 Tanzania, the arthritogenic Chikungunya virus (CHIKV) re-emerged globally 2005, leading to widespread outbreaks. Unlike other arboviruses, CHIKV predominantly induces symptomatic infections (72-96%), marked by fever, myalgia, polyarthralgia, and rash. Although rarely fatal, atypical forms such as encephalopathies can occur. Notably, 75.4% of patients experience persistent arthralgias for up three years after acute phase. Understanding CHIKV’s pathophysiology...
Zika virus (ZIKV) is a mosquito-borne flavivirus that emerged in the Pacific islands 2007 and spread to Americas 2015. The infection remains asymptomatic most cases but can be associated with severe neurological disorders. Despite massive efforts, no specific drug or vaccine against ZIKV available date. Claudins are tight-junction proteins favor entry of several flaviviruses, including ZIKV. In this study, we identified two peptides derived from N-terminal sequences claudin-7 claudin-1,...
Human T-Lymphotropic Virus type 1 (HTLV-1) is endemic among people of Melanesian descent in Papua New Guinea, Solomon Islands and Vanuatu, Indigenous populations from Central Australia. Molecular studies revealed that these Australo-Melanesian strains constitute the highly divergent HTLV-1c subtype. Caledonia a French overseas territory located Southwest Pacific Ocean. HTLV-1 situation poorly documented molecular epidemiology infection remains unknown.Studying 500 older adults natives...
Zika virus (ZIKV) belongs to the large category of arboviruses. Surprisingly, several human-to-human transmissions ZIKV have been notified, either following sexual intercourse or from mother fetus during pregnancy. Importantly, high viral loads detected in human breast milk infected mothers, and existence breastfeeding as a new mode mother-to-child transmission was recently hypothesized. However, maternal origin infectious particles is currently unknown. Here, we show that disseminates...
Zika virus (ZIKV) belongs to the Flavivirus genus in Flaviviridae family. Mainly transmitted via mosquito bites (Aedes aegypti, Aedes albopictus), ZIKV has been classified large category of arthropod-borne viruses, or arboviruses. However, during past two outbreaks French Polynesia (2013-2014) and Latin America (2015-2016), several cases human-to-human transmission were reported, either vertically transplacental route but also horizontally after sexual intercourse. Interestingly, high viral...
Zika virus (ZIKV) infection has been associated with a series of neurological pathologies. In patients ZIKV-induced disorders, the is detectable in central nervous system. Thus, ZIKV capable neuroinvasion, presumably through endothelial cells that constitute blood-brain barrier (BBB). We demonstrate susceptibility BBB to modulated by expression tight-junction protein claudin-7 (CLDN7). Downregulation CLDN7 reduced viral RNA yield, production, and release infectious particles several cell...
Investigations of cellular responses to viral infection are commonly performed on mixed populations infected and uninfected cells or using single-cell RNA sequencing, leading inaccurate low-resolution gene expression interpretations. Here, we deep polyA+ transcriptome analyses novel profiling severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) lung epithelial cells, sorted based the spike (S) protein. Infection caused a massive reduction in mRNAs long non-coding RNAs (lncRNAs),...
Japanese encephalitis virus (JEV) is the major cause of viral in South East Asia. It has been suggested that, as a consequence inflammatory process during JEV infection, there disruption blood-brain barrier (BBB) tight junctions that turn allows access to central nervous system (CNS). However, what happens at early times contact with BBB poorly understood. In present work, we evaluated ability both virulent and vaccine strain (JEV RP9 SA14-14-2, respectively) cross an vitro human model....
Zika virus (ZIKV) is a mosquito-borne RNA belonging to the Flavivirus genus of Flaviviridae family. During 60 years following its discovery in 1947, ZIKV caused little concern for public health as associated infection was reported mostly asymptomatic or inducing mild symptoms. However, since 2013, severe neurological symptoms have been with infection, compelling World Health Organization declare Public Emergency International Concern. Among those symptoms, birth defects may affect children...
Investigations of cellular responses to viral infection are commonly performed on mixed populations infected and uninfected cells or using single-cell RNA sequencing, leading inaccurate low-resolution gene expression interpretations. Here, we deep polyA+ transcriptome analyses novel profiling SARS-CoV-2 lung epithelial cells, sorted based the spike (S) protein. Infection caused a massive reduction in mRNAs lncRNAs, including transcripts coding for antiviral factors, such as interferons...
In several areas of high endemicity, HTLV-1 can be transmitted from mother to child, through prolonged breast-feeding. This way transmission is particularly linked the development Adult T Cell Leukemia (ATL). this context, we studied mechanisms digestive tract. We previously demonstrated in an vitro model compartimentalized co-culture device human enterocytes, lymphocytes and dendritic cells, that was unable infect or disrupt tight cell junctions, but could rather transported by transcytosis...
HAM/TSP is a neurodegenerative disease that develops upon infiltration of HTLV-1-infected lymphocytes into the central nervous system (CNS). Physiologically, CNS protected and isolated from immune by structure called blood-brain barrier (BBB). Thus have capacity to cross The mechanisms such crossing are still poorly understood. In context multiple sclerosis neuro-AIDS, Activated Leukocyte Cell Adhesion Molecule (ALCAM) was shown amplify leukocyte extravasation. earlier, ALCAM over expressed...