A5054257912- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- interferon and immune responses
- Plant and Fungal Interactions Research
- Molecular Biology Techniques and Applications
- RNA regulation and disease
- Peptidase Inhibition and Analysis
- Epigenetics and DNA Methylation
- Phagocytosis and Immune Regulation
- CRISPR and Genetic Engineering
- MicroRNA in disease regulation
- Cholangiocarcinoma and Gallbladder Cancer Studies
Centre National de la Recherche Scientifique
2019-2024
Dynamique de l'information génétique : bases fondamentales et cancer
2019-2024
Institut Curie
2018-2024
Université Paris Sciences et Lettres
2018-2024
Sorbonne Université
2019-2024
Université Paris Cité
2023
Cancer Research Center
2023
University of Antwerp
2023
Living Systems (United States)
2023
University of Exeter
2023
mRNA translation and decay appear often intimately linked although the rules of this interplay are poorly understood. In study, we combined our recent P-body transcriptome with transcriptomes obtained following silencing broadly acting repression factors, available CLIP related data. This revealed central role GC content in fate, terms localization, stability: P-bodies contain mostly AU-rich mRNAs, which have a particular codon usage associated low protein yield; GC-rich transcripts tend to...
Cholangiocarcinoma is a devastating liver cancer characterized by high aggressiveness and therapy resistance, resulting in poor prognosis. Long non-coding RNAs signals imposed oncogenic pathways, such as transforming growth factor β (TGFβ), frequently contribute to cholangiocarcinogenesis. Here, we explore novel effectors of TGFβ signalling cholangiocarcinoma. LINC00313 identified target gene. Gene expression genome-wide chromatin accessibility profiling reveal that nuclear transcriptionally...
Article6 May 2021Open Access Source DataTransparent process HOTAIR lncRNA promotes epithelial–mesenchymal transition by redistributing LSD1 at regulatory chromatin regions Julien Jarroux ncRNA, Epigenetic and Genome Fluidity, CNRS UMR3244, Sorbonne Université, PSL University, Institut Curie, Centre de Recherche, Paris, FranceThese authors contributed equally to this work as first Search for more papers author Dominika Foretek orcid.org/0000-0002-2231-1710 Claire Bertrand Marc Gabriel France...
tRNA is essential for translation and decoding of the proteome. The yeast proteome responds to stress biosynthesis contributes in this response by repression transcription alterations modification. Here we report that also involves processing pre-tRNA 3′ termini. By a combination Northern analyses RNA sequencing, show upon shift elevated temperatures and/or glycerol-containing medium, aberrant pre-tRNAs accumulate cells. For UAU Ile UUU Lys these forms are unprocessed at 5′ ends, but they...
Antisense long noncoding (aslnc)RNAs are extensively degraded by the nuclear exosome and cytoplasmic exoribonuclease Xrn1 in budding yeast Saccharomyces cerevisiae , lacking RNAi. Whether ribonuclease III Dicer affects aslncRNAs close RNAi-capable relatives remains unknown. Using genome-wide RNA profiling, here we show that primarily targeted Naumovozyma castellii only affecting Xrn1-sensitive levels Xrn1-deficient cells. The dcr1 xrn1 mutants display synergic growth defects, indicating...
In eukaryotic cells tRNA synthesis is negatively regulated by the protein Maf1, conserved from yeast to humans. Maf1 Saccharomyces cerevisiae mediates repression of trna transcription when are transferred medium with glucose glycerol, a non-fermentable carbon source. The strain deleted gene encoding (maf1Δ) viable but accumulates precursors. this study precursors were analysed RNA-Seq and Northern hybridization in wild type maf1Δ mutant grown or upon shift repressive conditions. A negative...
Investigations of cellular responses to viral infection are commonly performed on mixed populations infected and uninfected cells or using single-cell RNA sequencing, leading inaccurate low-resolution gene expression interpretations. Here, we deep polyA+ transcriptome analyses novel profiling severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) lung epithelial cells, sorted based the spike (S) protein. Infection caused a massive reduction in mRNAs long non-coding RNAs (lncRNAs),...
Summary Control of protein expression results from the fine tuning mRNA synthesis, decay and translation. These processes, which are controlled by a large number RNA-binding proteins localization in RNP granules such as P-bodies, appear often intimately linked although rules this interplay not well understood. In study, we combined our recent P-body transcriptome with various transcriptomes obtained following silencing broadly acting repression factors. This analysis revealed central role GC...
Abstract Some long noncoding (lnc)RNAs harbor the potential to produce functional micropeptides. Despite increasing recognition of their significance, regulatory dynamics cytoplasmic lncRNA expression, decay, and translation remain poorly understood. Here, we investigate role ribonucleases in controlling levels lncRNAs. By transcriptomic analysis identified DIS3 but not XRN1 as a major enzyme preventing accumulation lncRNAs cytoplasm. Single-molecule experiments illustrate an example...
ABSTRACT Antisense (as)lncRNAs are extensively degraded by the nuclear exosome and cytoplasmic exoribonuclease Xrn1 in budding yeast Saccharomyces cerevisiae , lacking RNA interference (RNAi). Whether ribonuclease III Dicer affects aslncRNAs close RNAi-capable relatives remains unknown. Using genome-wide profiling, here we show that primarily targeted Naumovozyma castellii only affecting Xrn1-sensitive lncRNAs (XUTs) levels Xrn1-deficient cells. The dcr1 xrn1 mutants display synergic growth...
Investigations of cellular responses to viral infection are commonly performed on mixed populations infected and uninfected cells or using single-cell RNA sequencing, leading inaccurate low-resolution gene expression interpretations. Here, we deep polyA+ transcriptome analyses novel profiling SARS-CoV-2 lung epithelial cells, sorted based the spike (S) protein. Infection caused a massive reduction in mRNAs lncRNAs, including transcripts coding for antiviral factors, such as interferons...
Abstract Investigations of cellular responses to viral infection are commonly performed on mixed populations infected and uninfected cells or using single-cell RNA sequencing, leading inaccurate low-resolution gene expression interpretations. Here, we deep polyA + transcriptome analyses novel profiling SARS-CoV-2 lung epithelial cells, sorted based the spike (S) protein. Infection caused a massive reduction in mRNAs lncRNAs, including transcripts coding for antiviral factors, such as...
Abstract Around 12% of multiple myeloma (MM) cases harbour mutations in DIS3 , which encodes an RNA decay enzyme that controls the turnover some long noncoding RNAs (lncRNAs). Although lncRNAs, by definition, do not encode proteins, can be a source (poly)peptides with biological importance, such as antigens. The extent and activities these “coding” lncRNAs MM are largely unknown. Here, we showed depletion results accumulation cytoplasm 5162 DIS3-sensitive transcripts (DISTs) previously...
Abstract Some of the long noncoding (lnc)RNAs harbor potential to produce functional micro peptides. Despite their increasing significance, regulatory dynamics cytoplasmic lncRNA expression, decay, and translation remain poorly understood. Here, we investigate role ribonucleases in controlling levels lncRNAs. We observed large accumulation a previously assumed nuclear-localized DIS3-sensitive transcripts (DISTs), contrary low number lncRNAs sensitive XRN1. Approximately 14.5% (DISTs) contain...
Summary Epithelial-to-mesenchymal transition (EMT) drives a loss of epithelial traits by neoplastic cells enabling metastasis and recurrence in cancer. HOTAIR emerged as one the most renowned long noncoding RNAs promoting EMT mostly scaffold for PRC2 repressive histone H3 Lys27 methylation at gene promoters. In addition to PRC2, interacts with Lsd1 lysine demethylase, known epigenetic regulator cell fate during development differentiation. However, role function is still poorly understood....
Abstract Triple Negative Breast Cancers (TNBCs) are the most aggressive breast cancers and so far, benefited very little from personalized medicine. Unlike in hormone positive cancers, coding transcriptomic signatures like Lehmann’s failed to predict which TNBC patients will benefit perioperative chemotherapy. Here, we analyzed total transcriptome of a unique cohort 44 tumors before neoadjuvant chemotherapy (NAC), including both that were chemosensitive (N=26) chemoresistant (N=18) following...