A5077580993- Mosquito-borne diseases and control
- Virus-based gene therapy research
- interferon and immune responses
- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Virology and Viral Diseases
- Viral Infections and Vectors
- Respiratory viral infections research
- Animal Virus Infections Studies
- Biochemical and Molecular Research
- Viral Infections and Immunology Research
- Immune Cell Function and Interaction
- HIV Research and Treatment
- Immunotherapy and Immune Responses
- Bioinformatics and Genomic Networks
- SARS-CoV-2 and COVID-19 Research
- Animal Disease Management and Epidemiology
- Immune Response and Inflammation
- Influenza Virus Research Studies
- Viral gastroenteritis research and epidemiology
- RNA and protein synthesis mechanisms
- Hepatitis B Virus Studies
- RNA Research and Splicing
- Endoplasmic Reticulum Stress and Disease
- Autophagy in Disease and Therapy
Université Claude Bernard Lyon 1
2000-2024
École Normale Supérieure de Lyon
1997-2024
Institut Pasteur
2012-2024
Centre National de la Recherche Scientifique
2015-2024
Centre International de Recherche en Infectiologie
2019-2024
Inserm
2000-2024
Laboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques
2015-2024
Université Paris Cité
2015-2023
Université de Lyon
2022
Virologie et Pathologies Humaines
2021
To initiate studies on how protein-protein interaction (or "interactome") networks relate to multicellular functions, we have mapped a large fraction of the Caenorhabditis elegans interactome network. Starting with subset metazoan-specific proteins, more than 4000 interactions were identified from high-throughput, yeast two-hybrid (HT=Y2H) screens. Independent coaffinity purification assays experimentally validated overall quality this Y2H data set. Together already described and interologs...
A proteome-wide mapping of interactions between hepatitis C virus (HCV) and human proteins was performed to provide a comprehensive view the cellular infection. total 314 protein-protein HCV identified by yeast two-hybrid 170 literature mining. Integration this data set into reconstructed interactome showed that interacting with are enriched in highly central interconnected proteins. global analysis on basis functional annotation highlighted enrichment pathways targeted HCV. network...
The advent of systems biology necessitates the cloning nearly entire sets protein-encoding open reading frames (ORFs), or ORFeomes, to allow functional studies corresponding proteomes. Here, we describe generation a first version human ORFeome using newly improved Gateway recombinational approach. Using Mammalian Gene Collection (MGC) resource as starting point, report successful 8076 ORFs, representing at least 7263 genes, mini-pools PCR-amplified products. These were assembled into 1.1...
Autophagy is a conserved degradative pathway used as host defense mechanism against intracellular pathogens. However, several viruses can evade or subvert autophagy to insure their own replication. Nevertheless, the molecular details of viral interaction with remain largely unknown. We have determined ability 83 proteins families RNA (Paramyxoviridae, Flaviviridae, Orthomyxoviridae, Retroviridae and Togaviridae), interact 44 human autophagy-associated using yeast two-hybrid bioinformatic...
Human coronaviruses are associated with upper respiratory tract infections that occasionally spread to the lungs and other organs. Although airway epithelial cells represent an important target for infection, epithelium is also composed of elaborate network dendritic (DCs) essential sentinels immune system, sensing pathogens presenting foreign antigens T lymphocytes. In this report, we show in vitro infection by human coronavirus 229E (HCoV-229E) induces massive cytopathic effects DCs,...
Searching for stimulators of the innate antiviral response is an appealing approach to develop novel therapeutics against viral infections. Here, we established a cell-based reporter assay identify compounds stimulating expression interferon-inducible genes. DD264 was selected out 41,353 both its immuno-stimulatory and properties. While searching mode action, identified as inhibitor pyrimidine biosynthesis pathway. This metabolic pathway recently prime target broad-spectrum molecules, but...
Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus that has been responsible for an epidemic outbreak of unprecedented magnitude in recent years. Since then, significant efforts have made to better understand the biology this virus, but we still poor knowledge CHIKV interactions with host cell components at molecular level. Here describe extensive use high-throughput yeast two-hybrid (HT-Y2H) assays characterize between and human proteins. A total 22 high-confidence interactions,...
Dengue virus (DENV) infections cause the most prevalent mosquito-borne viral disease worldwide, for which no therapies are available. DENV encodes seven non-structural (NS) proteins that co-assemble and recruit poorly characterized host factors to form replication complex essential infection. Here, we provide a global proteomic analysis of human interact with NS1 protein. Combined functional RNAi screen, this study reveals comprehensive network cellular processes involved in infection...
Abstract EOMES and T-BET are related T-box transcription factors that control natural killer (NK) cell development. Here we demonstrate regulate largely distinct gene sets during this process. is dominantly expressed in immature NK cells drives early lineage specification by inducing hallmark receptors functions. By contrast, dominant mature cells, where it induces responsiveness to IL-12 represses the cycle, likely through transcriptional repressors. Regardless, many genes with functions...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of COVID-19 pandemic, which has led to a devastating global health crisis. The emergence variants that escape neutralizing responses emphasizes urgent need deepen our understanding SARS-CoV-2 biology. Using comprehensive identification RNA-binding proteins (RBPs) by mass spectrometry (ChIRP-MS) approach, we identify 107 high-confidence cellular factors interact with genome during infection. By systematically...
ABSTRACT Measles virus infection induces a profound immunosuppression that can lead to serious secondary infections. Here we demonstrate measles tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) mRNA and protein expression in human monocyte-derived dendritic cells. Moreover, virus-infected cells are shown be cytotoxic via the TRAIL pathway.
Measles virus (MV) infection induces a profound immunosuppression responsible for high rate of mortality in malnourished children. MV can encounter human dendritic cells (DCs) the respiratory mucosa or secondary lymphoid organs. The purpose this study was to investigate consequences DC by MV, particularly concerning their maturation and ability generate CD8+ T cell proliferation. We first show that MV-infected Langerhans monocyte-derived DCs undergo process similarly one induced TNF-alpha...
Chikungunya virus (CHIKV) is a mosquito-transmitted Alphavirus that causes in humans an acute infection characterized by polyarthralgia, fever, myalgia, and headache. Since 2005 this has been responsible for epidemic outbreak of unprecedented magnitude. By analogy with other alphaviruses, it thought cellular proteases are able to process the viral precursor protein E3E2 produce receptor-binding E2 associates as heterodimer E1. Destabilization exposure low pH allows fusion infection. We show...
The mitochondria-bound adapter MAVS participates in IFN induction by recruitment of downstream partners such as members the TRAF family, leading to activation NF-kappaB, and IRF3 pathways. A yeast two-hybrid search for MAVS-interacting proteins yielded Polo-box domain (PBD) mitotic Polo-like kinase PLK1. We showed that PBD associates with two different domains both dependent independent phosphorylation events. phosphodependent association requires phosphopeptide binding ability PBD. It takes...