A5000278296- HIV Research and Treatment
- Autophagy in Disease and Therapy
- HIV/AIDS drug development and treatment
- Mosquito-borne diseases and control
- Immune Cell Function and Interaction
- RNA Interference and Gene Delivery
- COVID-19 Clinical Research Studies
- Advanced biosensing and bioanalysis techniques
- Immunotherapy and Immune Responses
- Cytomegalovirus and herpesvirus research
- Toxoplasma gondii Research Studies
- Virology and Viral Diseases
- COVID-19 Impact on Reproduction
- Extracellular vesicles in disease
- Adenosine and Purinergic Signaling
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- SARS-CoV-2 and COVID-19 Research
- Plant Virus Research Studies
- Long-Term Effects of COVID-19
- Research on Leishmaniasis Studies
- Monoclonal and Polyclonal Antibodies Research
- interferon and immune responses
- SARS-CoV-2 detection and testing
- Calcium signaling and nucleotide metabolism
- Plant responses to water stress
Laboratoire de Biologie et Pharmacologie Appliquée
2019-2025
Université Paris-Saclay
2018-2025
Centre National de la Recherche Scientifique
2013-2023
Biologie cellulaire et Cancer
2023
Inserm
2009-2022
École Normale Supérieure - PSL
2022
Centre d'Immunologie et des Maladies Infectieuses
2016-2022
Sorbonne Université
2016-2022
Centre National pour la Recherche Scientifique et Technique (CNRST)
2019
École Normale Supérieure Paris-Saclay
2018-2019
Autophagy is a conserved degradative pathway used as host defense mechanism against intracellular pathogens. However, several viruses can evade or subvert autophagy to insure their own replication. Nevertheless, the molecular details of viral interaction with remain largely unknown. We have determined ability 83 proteins families RNA (Paramyxoviridae, Flaviviridae, Orthomyxoviridae, Retroviridae and Togaviridae), interact 44 human autophagy-associated using yeast two-hybrid bioinformatic...
The interplay between autophagy and intracellular pathogens is intricate as an essential cellular response to fight against infections, whereas numerous microbes have developed strategies escape this process or even exploit it their own benefit. fine tuned timing and/or selective molecular pathways involved in the induction of upon infections could be cornerstone allowing cells either control pathogens, invaded by them. We report here that measles virus infection induces successive...
Abstract It is widely assumed that CD4+ T cells recognize antigenic peptides (epitopes) derived solely from incoming, exogenous, viral particles or proteins. However, alternative sources of MHC class II (MHC-II)–restricted Ags have been described, in particular epitopes newly synthesized proteins (so-called endogenous). In this study, we show HIV-infected dendritic (DC) present MHC-II–restricted endogenous to HIV-specific (HS) cells. This pathway functions independently the exogenous route...
There is a strong demand for new and efficient antiviral compounds. A series of 2-hydroxy-1,4-naphthoquinone Mannich bases were screened their HIV-1-RNase H inhibitory activity. An assay was used to study the RNase inhibition by test Docking active derivatives into site enzyme carried out. Compounds 1e 2k showed distinctly higher activity (IC50 = 2.8–3.1 µM) than known inhibitors RDS1759 compound 13. The binding mode possible interactions with determined using molecular docking, which led...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the infectious agent that has caused current disease (COVID) pandemic. Viral infection relies on viral S (spike) protein/cellular receptor ACE2 interaction. Disrupting this interaction would lead to early blockage of replication. To identify chemical tools further study these functional interfaces, 139,146 compounds from different libraries were screened through an S/ACE2
Purinergic receptors and NOD-like receptor protein 3 (NLRP3) inflammasome regulate inflammation viral infection, but their effects on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection remain poorly understood. Here, we report that the purinergic P2X7 NLRP3 are cellular host factors required for SARS-CoV-2 infection. Lung autopsies from patients with disease 2019 (COVID-19) reveal expression is increased in targets of including alveolar macrophages, type II pneumocytes...
HIV-specific broadly neutralizing antibodies (bnAbs) have been isolated from patients with high viremia but also HIV controllers that repress HIV-1 replication. In these elite (ECs), multiple parameters contribute to viral suppression, including genetic factors and immune responses. Defining the correlates associated generation of bnAbs may help in designing efficient immunotherapies. this study, ECs either positive or negative for HLA-B*57 protective allele, treated HIV-infected...
Integration of the reverse-transcribed genome is a critical step retroviral life cycle. Strand-transfer inhibitors (INSTIs) used for antiretroviral therapy inhibit integration but can lead to resistance mutations in integrase gene, enzyme involved this reaction. A significant proportion INSTI treatment failures, particularly those with second-generation INSTIs, show no mutation gene. Here, we that replication selected dolutegravir-resistant virus 3'-PPT (polypurine tract) was effective,...
A variety of signals influence the capacity dendritic cells (DCs) to mount potent antiviral cytotoxic T-cell (CTL) responses. In particular, innate immune sensing by pathogen recognition receptors, such as TLR and C-type lectines, influences DC biology affects their susceptibility HIV infection. Yet, whether combined effects PPRs triggering infection HIV-specific (HS) CTL responses remain enigmatic. Here, we dissect impact receptors on maturation, infection, quality HS activation....
The emerging SARS-CoV-2 virus has affected the entire world with over 600 million confirmed cases and 6.5 deaths as of September 2022. Since beginning pandemic, several variants have emerged, different infectivity virulence. Several studies suggest an important role neutrophils in SARS-Cov-2 infection severity, but data about direct activation by is scarce. Here, we studied vitro human concern (VOCs). In our work, show that upon stimulation infectious particles, healthy resting upregulate...
Article10 October 2022Open Access Source DataTransparent process The Autophagy Receptor TAX1BP1 (T6BP) improves antigen presentation by MHC-II molecules Gabriela Sarango orcid.org/0000-0003-3929-7895 Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France Sorbonne Université, INSERM, Center Immunology and Microbial Infections (CIMI-Paris), Paris, Contribution: Formal analysis, Investigation, Methodology, Writing - review & editing...
Infection by retroviruses as HIV-1 requires the stable integration of their genome into host cells. This process needs formation integrase (IN)-viral DNA complexes, called intasomes, and interaction with target wrapped around nucleosomes within cell chromatin. To provide new tools to analyze this association select drugs, we applied AlphaLISA technology complex formed between prototype foamy virus (PFV) intasome nucleosome reconstituted on 601 Widom sequence. system allowed us monitor both...
ABSTRACT Retroviral integration into cell chromatin requires the formation of integrase-viral DNA complexes, called intasomes, and their interaction with target wrapped around nucleosomes. To further study this mechanism we developed an alphaLISA approach using prototype foamy virus (PFV) intasome human nucleosome. This system allowed us to monitor association between both partners investigate protein/protein protein/DNA interactions engaged in chromatin. Using approach, next screened...
Abstract CD4 + T lymphocytes play a major role in the establishment and maintenance of immunity. They are activated by antigenic peptides derived from extracellular or newly synthesized (endogenous) proteins presented on surface antigen presenting cells (APCs) MHC-II molecules. The pathways leading to endogenous presentation remain poorly characterized. We demonstrate here that autophagy receptor, T6BP, influences both autophagy-dependent -independent HIV- HCMV-derived peptides. By studying...
Abstract Purinergic receptors and NOD-like receptor protein 3 (NLRP3) inflammasome regulate inflammation viral infection, but their effects on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection remain poorly understood. Here, we report that the purinergic P2X7 NLRP3 are cellular host factors required for SARS-CoV-2 infection. Lung autopsies from patients with disease 2019 (COVID-19) reveal expression is increased in targets of including alveolar macrophages, type II...