A5014350676- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- Immune Cell Function and Interaction
- Silicon and Solar Cell Technologies
- Reproductive System and Pregnancy
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Thin-Film Transistor Technologies
- Nanowire Synthesis and Applications
- Solidification and crystal growth phenomena
- Semiconductor materials and interfaces
- Autophagy in Disease and Therapy
- CAR-T cell therapy research
- Herpesvirus Infections and Treatments
- Peptidase Inhibition and Analysis
- Advanced Surface Polishing Techniques
- Cardiac Valve Diseases and Treatments
- Nanofabrication and Lithography Techniques
- Protein Degradation and Inhibitors
- Silicon Nanostructures and Photoluminescence
- Nanoplatforms for cancer theranostics
- RNA Interference and Gene Delivery
- HIV Research and Treatment
- Photovoltaic System Optimization Techniques
- Optical Coatings and Gratings
University of Tübingen
2017-2025
Access
2021-2023
St Thomas' Hospital
2021
Natural and Medical Sciences Institute
2019-2020
Forschungszentrum Jülich
2013-2015
Abstract The classical HLA-C and the nonclassical HLA-E HLA-G molecules play important roles both in innate adaptive immune system. Starting already during embryogenesis continuing throughout our lives, these three Ags exert major functions tolerance, defense against infections, anticancer responses. Despite roles, identification characterization of peptides presented by has been lacking behind more abundant HLA-A HLA-B gene products. In this study, we elucidated peptide specificities HLA...
Personalized multipeptide vaccines are currently being discussed intensively for tumor immunotherapy. In order to identify epitopes—short, immunogenic peptides—suitable eliciting a tumor-specific immune response, human leukocyte antigen-presented peptides isolated by immunoaffinity purification from cancer tissue samples and analyzed liquid chromatography-coupled tandem mass spectrometry (LC–MS/MS). Here, we present MHCquant, fully automated, portable computational pipeline able process...
Enteroviruses (EVs), such as Coxsackievirus B5 (CVB5), are linked to pancreatic beta cell autoimmunity in type 1 diabetes (T1D). CVB5 infection of cells blocks their cap-dependent translation but spares cap-independent insulin secretory granule (SG) cargoes, including major T1D autoantigens. Despite this, SG stores depleted. We show that protease 2A rapidly depletes Golgi-associated sorting factor GGA2 due its short half-life. Immunostaining pancreas sections from recent-onset donors...
Glioblastoma is an aggressive primary brain tumor with bad prognosis. On the other hand, oncolytic measles virus (MeV) therapy experimental glioma treatment strategy clinical safety and first evidence of anti-tumoral efficacy. Therefore, we investigated combination MeV conventional therapies by cytotoxic survival assays in long-term cell lines LN229, LNZ308, stem-like GS8 cells, as well basal viral infectivity glioblastoma cultures T81/16, T1094/17, T708/16. We employed Chou-Talalay analysis...
For more than two decades naturally presented, human leukocyte antigen (HLA)-restricted peptides (immunopeptidome) have been eluted and sequenced using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Since, identified disease-associated HLA ligands characterized evaluated as potential active substances. Treatments based on HLA-presented shown promising results in clinical application personalized T cell-based immunotherapy. Peptide vaccination cocktails are produced...
Background Patients with cancers that exhibit extraordinarily high somatic mutation numbers are ideal candidates for immunotherapy and enable identifying tumor-specific peptides through stimulation of tumor-reactive T cells (Tc). Methods Colorectal (CRC) HROC113 HROC285 were selected based on TMB, microsatellite instability HLA class I expression. Their ligandome was characterized using mass spectrometry, compared the ligand atlas I-binding affinity predicted. Cryptic identified...
The potency of viral vector-based vaccines depends on their ability to induce strong transgene-specific immune response without triggering anti-vector immunity. Previously, Orf virus (ORFV, Parapoxvirus) strain D1701-V was reported as a novel vector mediating protection against infections. short-lived ORFV-specific and the absence neutralizing antibodies enables repeated immunizations enhancement humoral responses inserted antigens. However, only limited information exists about induced...
Abstract The success of cancer immunotherapy relies on the induction an immunoprotective response targeting tumor antigens (TAs) presented MHC-I molecules. We demonstrated that splicing inhibitor isoginkgetin and its water-soluble non-toxic derivative IP2 act at production stage pioneer translation products (PTPs). showed increases PTP-derived antigen presentation in cells vitro impairs growth vivo. action is long-lasting dependent CD8 + T cell against TAs. observed repertoire displayed...
Viral diseases pose major threats to humans and other animals, including the billions of chickens that are an important food source as well a public health concern due zoonotic pathogens. Unlike typical mammals, histocompatibility complex (MHC) can confer decisive resistance or susceptibility many viral diseases. An iconic example is Marek’s disease, caused by oncogenic herpesvirus with over 100 genes. Classical MHC class I II molecules present antigenic peptides T lymphocytes, it has been...
Article10 October 2022Open Access Source DataTransparent process The Autophagy Receptor TAX1BP1 (T6BP) improves antigen presentation by MHC-II molecules Gabriela Sarango orcid.org/0000-0003-3929-7895 Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France Sorbonne Université, INSERM, Center Immunology and Microbial Infections (CIMI-Paris), Paris, Contribution: Formal analysis, Investigation, Methodology, Writing - review & editing...
Cytotoxic T lymphocytes are key for controlling viral infection. Unraveling CD8+ cell-mediated immunity to distinct influenza virus strains and subtypes across prominent HLA types is relevant combating seasonal infections emerging new variants. Using an immunopeptidomics approach, naturally presented A virus-derived ligands restricted HLA-A*24:02, HLA-A*68:01, HLA-B*07:02, HLA-B*51:01 molecules were identified. Functional characterization revealed multifunctional memory cell responses nine...
In this study, nanoimprint processing was used to realize various multiscale textures on glass substrates for application in thin-film photovoltaic devices. The are formed by a combination of large and small features, which proofed be beneficial light trapping silicon solar cells. Two approaches the fabrication presented study. first approach, texture is realized at lacquer/transparent conductive oxide (TCO) interface, second TCO/Si interface. Various types were fabricated tested...
Abstract Glioblastoma are incurable primary tumors of the central nervous system that frequently harbor molecular alterations in retinoblastoma pathway with subsequent cell cycle abnormalities. It is aimed to investigate anti‐glioma activity novel cycle‐stabilizing compound Argyrin F and its potential treatment‐induced vulnerabilities exploit possibilities for rational combination therapies. Human murine glioma cells used, cytotoxicity clonogenic survival assays, analyses, immunoblots...
In this work, we report on the prototyping of thin-film silicon tandem solar cells with periodic light-trapping textures. Our approach combines an industrial applicable nano-imprint process, for fabrication advanced light management concepts, state-of-the-art thin film cell technology. a joint experimental effort between project partners European “Fast-Track”, demonstrate that optimal nano-textures at front-side multi-junction enhance power conversion efficiency device in comparison to...
Two types of nano-imprinted 2D grating textures were tested on their light trapping performance for thin film Si tandem solar cells in combination with various TCO’s. The rough TCO layers like LPCVD ZnO leads to double textured superstrates that exhibit at least a similar capability as the state-of-the-art reference while using thinner layers. Further optimization AR air/glass interface has potential >13% devices and absorber
The structural and electrical properties of as grown multicrystalline (mc) solar silicon have been characterized with special emphasis on the ingot's edge regions. For this purpose a vertical cross section an mc-Si Bridgman ingot was investigated by Fourier transform infrared spectroscopy (FTIR), laser scattering tomography (LST), lateral photovoltage scanning (LPS), microscopy microwave detected photoconductivity decay (&PCD). Images distribution dislocations, grain boundaries,...
Abstract The success of cancer immunotherapy relies on the induction an immunoprotective response targeting tumor antigens (TAs) presented by cells MHC class I molecules. Alternative translation events emerged as a rich source TAs and generate so-called Pioneer Translation Products (PTPs), which are peptides generated from unspliced mRNA. We demonstrated in vitro vivo that splicing inhibitor isoginkgetin derived water-soluble less toxic molecule, IP2, act at production stage PTPs. showed IP2...