A5002929265- Immune Response and Inflammation
- Immune Cell Function and Interaction
- interferon and immune responses
- T-cell and B-cell Immunology
- Animal Virus Infections Studies
- Animal Nutrition and Physiology
- Herpesvirus Infections and Treatments
- Cytokine Signaling Pathways and Interactions
- Virology and Viral Diseases
- Microbial infections and disease research
- Animal Disease Management and Epidemiology
- Immunotherapy and Immune Responses
- Thyroid Disorders and Treatments
- Aquaculture disease management and microbiota
- Viral Infections and Vectors
- Gut microbiota and health
- Cytomegalovirus and herpesvirus research
- Probiotics and Fermented Foods
- Salmonella and Campylobacter epidemiology
- Monoclonal and Polyclonal Antibodies Research
- Influenza Virus Research Studies
- Clostridium difficile and Clostridium perfringens research
- Viral gastroenteritis research and epidemiology
- Animal health and immunology
- Antimicrobial Peptides and Activities
Ludwig-Maximilians-Universität München
2016-2025
Freie Universität Berlin
2007-2016
University Medical Center Freiburg
2014
Institute of Animal Physiology of the Slovak Academy of Sciences
1996-2006
LMU Klinikum
2002-2006
University of Freiburg
1995-2000
Swiss National Science Foundation
2000
Czech Academy of Sciences, Institute of Molecular Genetics
1996
Universität Innsbruck
1996
Beltsville Agricultural Research Center
1994
Significance Chickens have been an important animal model in the fields of developmental biology and immunology over last century contributed a number basic findings these areas. However, their use has limited more recently because there no way to genetically engineer specific mutations chicken. In addition research applications, genetic modification birds could also be used mitigate threat avian influenza by production flu-resistant bird stocks agriculture. Here we describe efficient target...
There is a high mortality rate in patients with systemic inflammatory response syndrome (SIRS) or sepsis. Therefore, an early diagnosis and prognostic assessment important for optimal therapeutic intervention. The objective of the study was to evaluate if baseline values changes serum C-reactive protein (CRP) might predict survival dogs SIRS sepsis.Prospective study; July 2004 2005.Small Animal Clinic, Berlin, Clinic Small Medicine, Munich.Sixty-one dogs.For CRP analysis blood drawn on day...
The immune system plays a crucial role in sustaining animal health. Its development is markedly influenced by early microbial colonization of the gastrointestinal tract.
Non-mammalian NK cells have not been characterized in detail; however, their analysis is essential for the understanding of cell receptor phylogeny. As a first step towards defining chicken cells, several tissues were screened presence phenotypically defined as CD8(+) lacking T- or B-lineage specific markers. By this criteria, approximately 30% intestinal intraepithelial lymphocytes (IEL), but <1% splenocytes peripheral blood cells. These CD8(+)CD3(-) IEL used generation 28-4 mAb,...
Upon induction with lipopolysaccharide (LPS) the chicken macrophage cell line HD‐11 secretes an activity that stimulates synthesis of a CXC chemokine in fibroblast CEC‐32. We used cDNA expression cloning strategy COS cells to characterize this activity. The isolated clone codes for polypeptide 267 amino acids which lacks hydrophobic N‐terminal domain could serve as secretory signal. Sequence homology and structural features indicate protein is homolog mammalian interleukin‐1β (ChIL‐1β)....
Suppression subtractive hybridization technology was used to identify differentially expressed genes in spleens of chickens that had been treated with the synthetic immune modifier S-28463. One induced chicken gene encoded a protein about 35% sequence identity human interleukin-6 (IL-6). It consists 241 amino acids including putative N-terminal signal peptide 47 residues. Bacterially IL-6 (ChIL-6) carrying histidine tag place biologically active: it proliferation IL-6-dependent murine...
Avian bornaviruses (ABV), representing a new genus within the family Bornaviridae, were recently discovered in parrots from North America and Israel with proventricular dilatation disease (PDD). We show here that closely related viruses are also present captive European of various species PDD. The six ABV strains we identified clinically diseased birds members previously defined genotypes 2 4. Viruses both readily established persistent, noncytolytic infections quail chicken cell lines but...
The Campylobacter jejuni-host interaction may be affected by the host's gut microbiota through competitive exclusion, metabolites, or modification of immune response. To understand this interaction, C. jejuni colonization and local responses were compared in chickens with different compositions. Birds treated an antibiotic cocktail (AT) (experiments 1 2) raised under germfree (GF) conditions (experiment 3). At 18 days posthatch (dph), they orally inoculated either 104 CFU diluent. Cecal as...
Tumor necrosis factor-α (TNF-α) is a pleiotropic cytokine playing critical roles in host defense and acute chronic inflammation. It has been described fish, amphibians mammals but was considered to be absent the avian genomes. Here we report on identification functional characterization of orthologue. The chicken TNF-α (chTNF-α) encoded by highly GC-rich gene, whose product shares with its mammalian counterpart 45% homology extracellular part displaying characteristic TNF domain. Orthologues...
By searching a chicken EST database, we identified cDNA clone that appeared to contain the entire open reading frame (ORF) of interleukin-18 (ChIL-18). The encoded protein consists 198 amino acids and exhibits approximately 30% sequence identity IL-18 humans various others mammals. Sequence comparisons reveals putative caspase-1 cleavage site at aspartic acid 29 primary translation product, indicating mature ChIL-18 might consist 169 acids. Bacterially expressed in which N-terminal precursor...
Abstract The phylogeny of Th1 and Th2 subsets has not been characterized mainly due to the limited information regarding cytokines in nonmammalian vertebrates. In this study, we characterize a Th1-like regulatory system focusing on IL-18-regulated IFN-γ secretion. Stimulation splenocytes with chicken IL-18 induced high levels Depletion either macrophages or CD4+ T cells from splenocyte cultures caused unresponsiveness IL-18. contrast, PBL were unresponsive presence absence macrophages, but...
Abstract Supernatants of the chicken T cell line 855 contain antiviral and macrophage‐activating factor activity strongly activate transcription guanylate‐binding protein (GBP) gene in cells. To characterize cytokine responsible for GBP‐inducing activity, we chose a cDNA expression cloning strategy COS Sequencing positive clone revealed that it encodes interferon‐γ (ChIFN‐γ). Histidine‐tagged ChIFN‐γ was expressed Escherichia coli purified by nickel chelate affinity chromatography. from...
Non-structural protein NS1 of influenza A virus counteracts the host immune response by blocking synthesis type I interferon (IFN). As deletion complete gene has to date been reported only in human H1N1 strain A/PR/8/34, it remained unclear whether is a non-essential virulence factor other strains as well. In this report, properties NS1-deficient mutants derived from SC35M (H7N7) are described. mutant that completely lacks was an excellent inducer IFN mammalian and avian cells culture and,...
The development, progression, and recurrence of autoimmune diseases are frequently driven by a group participatory autoantigens. We identified characterized novel autoantigens analyzing the autoantibody binding pattern from horses affected spontaneous equine recurrent uveitis to retinal proteome. Cellular retinaldehyde-binding protein (cRALBP) had not been described previously as autoantigen, but subsequent characterization in revealed B T cell autoreactivity this established link epitope...
Interferons (IFNs) are essential components of the antiviral defense system vertebrates. In mammals, functional receptors for type III IFN (lambda interferon [IFN-λ]) found mainly on epithelial cells, and IFN-λ was demonstrated to play a crucial role in limiting viral infections mucosal surfaces. To determine whether plays similar birds, we produced recombinant chicken (chIFN-λ) used replication-competent retroviral RCAS vector generate mosaic-transgenic embryos that constitutively express...