A5033417797- Cancer Cells and Metastasis
- 3D Printing in Biomedical Research
- Renal cell carcinoma treatment
- Monoclonal and Polyclonal Antibodies Research
- Cancer Genomics and Diagnostics
- Glioma Diagnosis and Treatment
- Helicobacter pylori-related gastroenterology studies
- Advanced Biosensing Techniques and Applications
- Immune cells in cancer
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Pluripotent Stem Cells Research
- Renal and related cancers
- Systemic Lupus Erythematosus Research
- Cancer Research and Treatments
- Systemic Sclerosis and Related Diseases
- Pancreatic and Hepatic Oncology Research
- Galectins and Cancer Biology
- Cancer Mechanisms and Therapy
- Prostate Cancer Treatment and Research
- Microfluidic and Bio-sensing Technologies
- Mitochondrial Function and Pathology
- Nanoplatforms for cancer theranostics
- CRISPR and Genetic Engineering
- Ferroptosis and cancer prognosis
Natural and Medical Sciences Institute
2016-2025
University of Tübingen
2014-2025
Health and Environmental Sciences Institute
2023
Reutlingen University
2020
Universitätsklinikum Tübingen
2019
Ludwig Cancer Research
2009-2012
Uppsala University
2009-2012
Science for Life Laboratory
2012
Technical University of Munich
2004-2007
Klinikum rechts der Isar
2004
Dense fluorophore labeling without compromising the biological target is crucial for genuine super-resolution microscopy. Here we introduce a broadly applicable strategy fixed and living cells utilizing short peptide tag-specific nanobody (BC2-tag/bivBC2-Nb). BC2-tagging of ectopically introduced or endogenous proteins does not interfere with examined structures bivBC2-Nb staining results in close-grained minimal linkage errors. This allowed us to perform high-quality dSTORM imaging various...
Pancreatic ductal adenocarcinoma (PDAC) is among the most aggressive human malignancies with an overall 5-year survival rate of <5%. Despite significant advances in treatment disease during past decade, median (∼6 months) has hardly improved, warranting need to identify novel targets for therapeutic approaches. Quantitative real time PCR, western blot analyses and immunohistochemical staining tissue microarrays were used analyse expression TTK gene primary PDAC tissues cell lines. To inhibit...
Chromobodies are intracellular nanoprobes that combine the specificity of antibodies with convenience live fluorescence imaging in a flexible, DNA-encoded reagent. Here, we present first application this technique to an intact living vertebrate organism. We generated zebrafish lines expressing chromobodies trace major cytoskeletal component actin and cell cycle marker PCNA spatial temporal specificity. Using these chromobodies, captured full localization dynamics endogenous antigens...
Abstract The integration of microfluidics and cell biology has reached a significant milestone with the development “organ-on-chips”, smart technological platforms that, once applied to study human diseases, such as cancer, might ultimately contribute design personalised treatments hence improve health outcomes. This paper reports that combination dielectrophoresis (DEP) allows culture different pancreatic ductal adenocarcinoma (PDAC) lines into cyclic olefin polymer (COP) chamber (HepaChip...
Glioblastoma is an aggressive primary tumor of the central nervous system. Targeting immunosuppressive glioblastoma-associated microenvironment interesting therapeutic approach. Tumor-associated macrophages represent abundant population tumor-infiltrating host cells with tumor-promoting features. The colony stimulating factor-1/ factor-1 receptor (CSF-1/CSF1R) axis plays important role for macrophage differentiation and survival. We thus aimed at investigating antiglioma activity CSF1R...
Abstract Background The metabolic enzyme nicotinamide‐N‐methyltransferase (NNMT) is highly expressed in various cancer entities, suggesting tumour‐promoting functions. We systematically investigated NNMT expression and its interactions clear cell renal carcinoma (ccRCC), a prominent RCC subtype with alterations, to elucidate role as drug target. Methods was assessed primary ccRCC ( n = 134), non‐tumour tissue ccRCC‐derived metastases 145) by microarray analysis and/or immunohistochemistry....
Abstract Background Registered systemic treatment options for glioblastoma patients are limited. The phase II REGOMA trial suggested an improvement of median overall survival in progressive by the multi-tyrosine kinase inhibitor regorafenib. This has not been confirmed GBM AGILE. So far, regorafenib administered as monotherapy or addition to standard care newly diagnosed glioblastoma. Rational combination therapies involving might be a reasonable strategy. Here, we aimed at identifying...
Previous studies showed that loss of the T-cell protein tyrosine phosphatase (TC-PTP) induces Rab4a-dependent recycling platelet-derived growth factor (PDGF) beta-receptor in mouse embryonic fibroblasts (MEFs). Here we identify kinase C (PKC) alpha as critical signaling component regulates sorting PDGF at early endosomes. Down-regulation PKC abrogated receptor by preventing activated into EGFP-Rab4a positive domains on This effect was mimicked inhibition PKCalpha, using myristoylated...
Receptor tyrosine kinase (RTK) signaling is frequently increased in tumor cells, sometimes as a result of decreased receptor down-regulation. The extent to which the endocytic trafficking routes can contribute such RTK hyperactivation unclear. Here, we show for first time that fibroblast transformation by H-RasG12V induces internalization platelet-derived growth factor β-receptor (PDGFRβ) macropinocytosis, enhancing its activity and increasing anchorage-independent proliferation. PDGFRβ...
// Giuseppina Sannino 1, 6 , Nicole Armbruster 7 Mona Bodenhöfer 1 Ursula Haerle Diana Behrens 2 Malte Buchholz 3 Ulrich Rothbauer 4 Bence Sipos 5 Christian Schmees Natural and Medical Sciences Institute (NMI) at the University of Tuebingen, Tumor Biology Group, Reutlingen, Germany Experimental Pharmacology Oncology GmbH, Berlin, Department Medicine, Division Gastroenterology, Endocrinology Metabolism, Philipps Marburg, Pharmaceutical Biotechnology, Pathology, Current address:...
In light of the frequent development therapeutic resistance in cancer treatment, there is a strong need for personalized model systems representing patient tumor heterogeneity, while enabling parallel drug testing and identification appropriate treatment responses individual patients. Using ovarian as prime example heterogeneous disease, we developed 3D preclinical comprised patient-derived microtumors (PDM) autologous tumor-infiltrating lymphocytes (TILs) to identify vulnerabilities...
HepaChip-MP: a 24-culture-chamber, automated microfluidic<italic>in vitro</italic>model of the liver sinusoid in multiwellplate format.
Endothelial and epithelial cellular barriers play a vital role in the selective transport of solutes other molecules. The properties function these are often affected case inflammation disease. Modelling vitro can greatly facilitate studies inflammation, disease mechanisms progression, addition, be exploited for drug screening discovery. Here, we report on parallelizable microfluidic platform multiwell plate format with ten independent cell culture chambers to support modelling co-cultured...
The DNA damage response (DDR) is a physiological network preventing malignant transformation, e.g. by halting cell cycle progression upon detection and promoting repair. Glioblastoma are incurable primary tumors of the nervous system DDR dysregulation contributes to acquired treatment resistance. Therefore, targeting promising therapeutic anti-glioma strategy. Here, we investigated Ataxia telangiectasia Rad3 related (ATR) inhibition (ATRi) functionally-instructed combination therapies...
Despite tremendous progress in deciphering breast cancer at the genomic level, pronounced intra- and intertumoral heterogeneity remains a major obstacle to advancement of novel more effective treatment approaches. Frequent failure development resistance highlight need for patient-derived tumor models that reflect individual tumors patients allow comprehensive analyses parallel functional validation individualized therapeutically targetable vulnerabilities protein signal transduction...
Abstract Glioblastoma are incurable primary tumors of the central nervous system that frequently harbor molecular alterations in retinoblastoma pathway with subsequent cell cycle abnormalities. It is aimed to investigate anti‐glioma activity novel cycle‐stabilizing compound Argyrin F and its potential treatment‐induced vulnerabilities exploit possibilities for rational combination therapies. Human murine glioma cells used, cytotoxicity clonogenic survival assays, analyses, immunoblots...
CD34+ cells were isolated from peripheral blood of a breast cancer patient. By the introduction five integration-free episomal vectors, successfully reprogrammed and resulted in four iPSC clones. Flow Cytometry, reverse transcriptase PCR immunocytochemistry confirm robust expression pluripotency factors concomitant loss exogenous reprogramming plasmids. The maintenance genomic integrity was confirmed by array-based comparative hybridization iPSCs harbored capacity to differentiate into all...
Despite intensive research, the development of endometriosis remains elusive. Highly painful symptoms, predominantly affecting young women, and limited therapeutic options highlight need to improve our understanding disease biology advance early diagnostic strategies.
Peripheral-blood derived CD34+ hematopoietic stem and progenitor cells were isolated from a 49-year old male donor successfully reprogrammed into human induced pluripotent (hiPSCs) using integration-free episomal vectors. The hiPSC line exhibited typical cell-like morphology endogenously expressed several pluripotency markers by concomitant loss of exogenous reprogramming Genomic integrity was confirmed microarray-based comparative genomic hybridization (array CGH). Further analysis affirmed...