A5071175939- Microtubule and mitosis dynamics
- Advanced Fluorescence Microscopy Techniques
- Genomics and Chromatin Dynamics
- Cellular Mechanics and Interactions
- Advanced Electron Microscopy Techniques and Applications
- Microbial Metabolic Engineering and Bioproduction
- Nuclear Structure and Function
- Biofuel production and bioconversion
- Advanced Biosensing Techniques and Applications
- Cell Image Analysis Techniques
- DNA Repair Mechanisms
- Photochromic and Fluorescence Chemistry
- Cardiomyopathy and Myosin Studies
- Meat and Animal Product Quality
- Photoreceptor and optogenetics research
- Enzyme Catalysis and Immobilization
- Microbial Inactivation Methods
- Proteins in Food Systems
- GABA and Rice Research
- Near-Field Optical Microscopy
- Single-cell and spatial transcriptomics
- bioluminescence and chemiluminescence research
- Bioeconomy and Sustainability Development
- Amino Acid Enzymes and Metabolism
- Supramolecular Self-Assembly in Materials
Acies Bio (Slovenia)
2024
Max Planck Institute for Terrestrial Microbiology
2016-2023
Loewe Center for Synthetic Microbiology
2016-2023
Dense fluorophore labeling without compromising the biological target is crucial for genuine super-resolution microscopy. Here we introduce a broadly applicable strategy fixed and living cells utilizing short peptide tag-specific nanobody (BC2-tag/bivBC2-Nb). BC2-tagging of ectopically introduced or endogenous proteins does not interfere with examined structures bivBC2-Nb staining results in close-grained minimal linkage errors. This allowed us to perform high-quality dSTORM imaging various...
Abstract Photoconversion of fluorescent proteins by blue and complementary near‐infrared light, termed primed conversion (PC), is a mechanism recently discovered for Dendra2. We demonstrate that controlling the conformation arginine at residue 66 threonine 69 from Anthozoan families (Dendra2, mMaple, Eos, mKikGR, pcDronpa protein families) represents general route to facilitate PC. Mutations alanine 159 or serine 173, which are known influence chromophore flexibility allow reversible...
The key to ensuring proper chromosome segregation during mitosis is the kinetochore (KT), a tightly regulated multiprotein complex that links centromeric chromatin spindle microtubules and as such leads process. Understanding its architecture, function, regulation therefore essential. However, due complexity dynamics, only individual subcomplexes could be studied in structural detail so far. In this study, we construct nanometer-precise situ map of human-like regional KT Schizosaccharomyces...
There is an ongoing search for sustainable and functional alternative protein sources to animal proteins. Methylobacillus flagellatus (grown on renewable methanol) known give high yields with low resource requirements. However, the techno-functionality food applications has not yet been explored. In this study, biomass was processed by microfluidizer, centrifugation acid precipitation. Unprocessed (UB: content of 73 %) fractions were investigated their composition, physicochemical...
Super-resolution fluorescence microscopy plays a major role in revealing the organization and dynamics of living cells. Nevertheless, single-molecule localization imaging multiple targets is still limited by availability suitable fluorophore combinations. Here, we introduce novel strategy which combines primed photoconversion (PC) UV-photoactivation for different molecular species tagged fluorescent protein In this approach, proteins can be specifically photoactivated/-converted light...
The actin cytoskeleton operates in a multitude of cellular processes including cell shape and migration, mechanoregulation, membrane or organelle dynamics. However, its filamentous properties functions inside the mammalian nucleus are less well explored. We previously described transient assembly at mitotic exit that promotes nuclear expansion during chromatin decondensation. Here, we identify non-muscle α-actinin 4 (ACTN4) as critical regulator to facilitate F-actin reorganization bundling...
Abstract Background Bacillus methanolicus is the next workhorse in biotechnology using methanol, an alternative and economical one-carbon feedstock that can be obtained directly from carbon dioxide, as both energy source for production of various value-added chemicals. The wild-type strain B. MGA3 naturally overproduces l -glutamate methanol-based fed-batch fermentations. Results Here we generated, by induced mutagenesis, evolved exhibiting enhanced capability (>150%). To showcase...
Abstract Photokonversion von fluoreszierenden Proteinen mithilfe blauem und komplementärem nah‐infraroten Licht, genannt “Primed Conversion” (PC), ist ein kürzlich für Dendra2 entdeckter Mechanismus. Die Kontrolle über die Konformation Arginin an Position 66 Threonin 69 fluoreszierender Proteine aus Anthozoa (Proteinfamilien Dendra2, mMaple, Eos, mKikGR, pcDronpa) ermöglicht in allgemeiner Weise PC. Mutationen Alanin 159 oder Serin 173, dafür bekannt sind, Chromophorflexibilität zu...
Abstract The key to ensuring proper chromosome segregation during mitosis is the kinetochore complex. This large and tightly regulated multi-protein complex links centromeric chromatin microtubules attached spindle pole body as such leads process. Understanding architecture, function regulation of this vital therefore essential. However, due its complexity dynamics, only individual subcomplexes could be studied in high-resolution structural detail so far. In study we construct a...
Abstract The actin cytoskeleton operates in a multitude of cellular processes including cell shape and migration, mechanoregulation, as well membrane or organelle dynamics. However, its filamentous properties functions inside the mammalian nucleus are less explored. We previously described transient assembly at mitotic exit that promotes nuclear expansion during chromatin decondensation. Here, we identify non-muscle ACTN4 critical regulator to facilitate F-actin formation, reorganization...