Timotheus Y. Althuis

ORCID: 0009-0009-8538-1941
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About
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Research Areas
  • Meat and Animal Product Quality
  • Microbial Inactivation Methods
  • CRISPR and Genetic Engineering
  • Vibrio bacteria research studies
  • Proteins in Food Systems
  • Bacterial Genetics and Biotechnology

Wageningen University & Research
2024

There is an ongoing search for sustainable and functional alternative protein sources to animal proteins. Methylobacillus flagellatus (grown on renewable methanol) known give high yields with low resource requirements. However, the techno-functionality food applications has not yet been explored. In this study, biomass was processed by microfluidizer, centrifugation acid precipitation. Unprocessed (UB: content of 73 %) fractions were investigated their composition, physicochemical...

10.1016/j.foodhyd.2024.109832 article EN cc-by Food Hydrocolloids 2024-02-02

ABSTRACT The editing of plasmids and construction plasmid libraries is paramount to the engineering desired functionalities in synthetic biology. Typically, with targeted mutations are produced through time– resource-consuming DNA amplification and/or cloning steps. In this study, we establish MOSAIC, a highly efficient protocol for generation combinatorial libraries. This quick employs single-stranded annealing protein (SSAP) CspRecT incorporate (libraries of) oligos harboring into target...

10.1101/2024.03.22.586135 preprint EN cc-by bioRxiv (Cold Spring Harbor Laboratory) 2024-03-22
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