A5087654889- Microbial Metabolic Engineering and Bioproduction
- Microbial Fuel Cells and Bioremediation
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Photosynthetic Processes and Mechanisms
- Microbial Community Ecology and Physiology
- Bacterial Genetics and Biotechnology
- Gene Regulatory Network Analysis
- RNA modifications and cancer
- Microbial metabolism and enzyme function
- Genomics and Phylogenetic Studies
- Algal biology and biofuel production
- Enzyme Structure and Function
- Metalloenzymes and iron-sulfur proteins
- CO2 Reduction Techniques and Catalysts
- Advanced biosensing and bioanalysis techniques
- Process Optimization and Integration
- thermodynamics and calorimetric analyses
- Viral Infectious Diseases and Gene Expression in Insects
- Enzyme Production and Characterization
- Metal-Catalyzed Oxygenation Mechanisms
- Mitochondrial Function and Pathology
- Biochemical and Molecular Research
Wageningen University & Research
2016-2025
Max Planck Institute of Molecular Plant Physiology
2018-2020
Abstract Six CO 2 fixation pathways are known to operate in photoautotrophic and chemoautotrophic microorganisms. Here, we describe chemolithoautotrophic growth of the sulphate-reducing bacterium Desulfovibrio desulfuricans (strain G11) with hydrogen sulphate as energy substrates. Genomic, transcriptomic, proteomic metabolomic analyses reveal that D. assimilates via reductive glycine pathway, a seventh pathway. In this is first reduced formate, which condensed second generate glycine....
Formate can be directly produced from CO2 and renewable electricity, making it a promising microbial feedstock for sustainable bioproduction. Cupriavidus necator is one of the few biotechnologically-relevant hosts that grow on formate, but uses Calvin cycle, high ATP cost which limits biomass product yields. Here, we redesign C. metabolism formate assimilation via synthetic, highly ATP-efficient reductive glycine pathway. First, demonstrate upper pathway segment supports biosynthesis...
Akkermansia muciniphila colonizes the mucus layer of gastrointestinal tract, where organism can be exposed to oxygen that diffuses from epithelial cells. To understand how A. is able survive and grow at this oxic-anoxic interface, its tolerance response reduction capacities were studied. was found tolerant. On top this, under aerated conditions, showed significant growth rate yield increased compared those seen strict anaerobic conditions. Transcriptome analysis revealed an initial stress...
Synthetic biology has brought about a conceptual shift in our ability to redesign microbial metabolic networks. Combining pathway-modularization with growth-coupled selection schemes is powerful tool that enables deep rewiring of the cell factories' biochemistry for rational bioproduction.
Abstract It has been known for decades that codon usage contributes to translation efficiency and hence protein production levels. However, its role in synthesis is still only partly understood. This lack of understanding hampers the design synthetic genes efficient production. In this study, we generated a synonymous codon-randomized library complete coding sequence red fluorescent protein. Protein levels full sequences were determined 1459 gene variants Escherichia coli. Using different...
One-carbon (C1) compounds such as methanol, formate, and CO2 are alternative, sustainable microbial feedstocks for the biobased production of chemicals fuels. In this study, we engineered carbon metabolism industrially important bacterium Pseudomonas putida to modularly assimilate these three substrates through reductive glycine pathway. First, demonstrated functionality C1-assimilation module by coupling growth auxotrophic strains formate assimilation. Next, extended in from...
To advance the sustainability of biobased economy, our society needs to develop novel bioprocesses based on truly renewable resources. The C1-molecule formate is increasingly proposed as carbon and energy source for microbial fermentations, it can be efficiently generated electrochemically from CO2 energy. Yet, its biotechnological conversion into value-added compounds has been limited a handful examples. In this work, we engineered natural formatotrophic bacterium C. necator cell factory...
High-level, recombinant production of membrane-integrated proteins in Escherichia coli is extremely relevant for many purposes, but has also been proven challenging. Here we study a combination transcriptional fine-tuning E. LEMO21(DE3) with different codon usage algorithms heterologous membrane proteins. The overexpression 6 compared the wild-type gene variant, commercially codon-optimized and codon-harmonized variant. We show that plays major role improving all tested Moreover, variants...
Different codon optimization algorithms are available that aim at improving protein production by optimizing translation elongation. In these algorithms, it is generally not considered how the altered coding sequence will affect secondary structure of corresponding RNA transcript, particularly effect on 5'-UTR and related ribosome binding site availability. This a serious drawback, because influence usage mRNA structures, especially near start gene, may strongly initiation. this study, we to...
Significance The Calvin cycle is the most important carbon fixation pathway in biosphere. However, its carboxylating enzyme Rubisco also accepts oxygen, thus producing 2-phosphoglycolate. Phosphoglycolate salvage pathways were extensively studied photoautotrophs but remain uncharacterized chemolithoautotrophs using cycle. Here, we study phosphoglycolate chemolithoautotrophic model bacterium Cupriavidus necator H16. We demonstrate that this mainly reassimilates 2-phosphoglycolate via...
Parageobacillus thermoglucosidasius is a thermophilic and facultatively anaerobic microbe, which emerging as one of the most promising model organisms for metabolic engineering. The use microorganisms industrial bioprocesses provides advantages increased reaction rates reduced cooling costs bioreactors compared to their mesophilic counterparts. Moreover, it enables starch or lignocellulose degradation fermentation occur at same temperature in Simultaneous Saccharification Fermentation (SSF)...