A5106416359- Microbial Metabolic Engineering and Bioproduction
- Amino Acid Enzymes and Metabolism
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- Biofuel production and bioconversion
- Microbial metabolism and enzyme function
- Polyamine Metabolism and Applications
- Microbial Natural Products and Biosynthesis
- Enzyme Catalysis and Immobilization
- Bacteriophages and microbial interactions
- CRISPR and Genetic Engineering
- Enzyme Production and Characterization
- Glycosylation and Glycoproteins Research
- Fungal Biology and Applications
- Algal biology and biofuel production
- Metabolism and Genetic Disorders
- Biochemical Acid Research Studies
- Fungal and yeast genetics research
- Antioxidant Activity and Oxidative Stress
- Probiotics and Fermented Foods
- Carbohydrate Chemistry and Synthesis
- Biochemical and Molecular Research
- Biopolymer Synthesis and Applications
- GABA and Rice Research
- Viral Infectious Diseases and Gene Expression in Insects
Norwegian University of Science and Technology
2015-2024
SINTEF
2006-2016
Abstract Background Production of recombinant proteins in bacteria for academic and commercial purposes is a well established field; however the outcomes process developments specific are still often unpredictable. One reason limited understanding performance expression cassettes relative to each other due different genetic contexts. Here we report results systematic study aiming at exclusively comparing commonly used regulator/promoter systems by standardizing designs replicon backbones....
Bacillus methanolicus can utilize methanol as the sole carbon source for growth and it encodes an NAD+-dependent dehydrogenase (Mdh), catalyzing oxidation of to formaldehyde. Recently, genomes B. strains MGA3 (ATCC53907) PB1 (NCIMB13113) were sequenced found harbor three different putative Mdh encoding genes, each belonging type III Fe-NAD+-dependent alcohol dehydrogenases. In strain, two these genes are encoded on chromosome one a plasmid; only chromosomal act gene previously described...
Summary Methanol is regarded as an attractive substrate for biotechnological production of value‐added bulk products, such amino acids and polyamines. In the present study, methylotrophic thermophilic bacterium B acillus methanolicus was engineered into a microbial cell factory platform chemical 1,5‐diaminopentane (cadaverine) from methanol. This achieved by heterologous expression E scherichia coli genes cad A ldc C encoding two different lysine decarboxylase enzymes, increasing overall L...
Abstract Bacteria are widely used as cell factories for production of enzymes and chemicals, mostly from sugars. Methylotrophic bacteria can utilize the one‐carbon compound methanol sole carbon source growth, metabolic engineering is being to develop bioprocesses based on these organisms conversion into value‐added chemicals. model strains include both Gram‐positive Gram‐negative in all cases metabolism proceeds via cell‐toxic intermediate formaldehyde. Thus, understanding genetics,...
We report the cloning and characterization of biosynthetic gene cluster (crtE, crtB, crtI, crtE2, crtYg, crtYh, crtX) γ-cyclic C(50) carotenoid sarcinaxanthin in Micrococcus luteus NCTC2665. Expression complete partial Escherichia coli hosts revealed that biosynthesis from precursor molecule farnesyl pyrophosphate (FPP) proceeds via C(40) lycopene, C(45) nonaflavuxanthin, flavuxanthin, sarcinaxanthin. Glucosylation was accomplished by crtX product. This is first describing pathway a...
Bacillus methanolicus can utilize methanol as its sole carbon and energy source, the scientific interest in this thermotolerant bacterium has focused largely on exploring potential a biocatalyst for conversion of into L-lysine L-glutamate. We present here genome sequences important B. model strain MGA3 (ATCC 53907) alternative wild-type PB1 (NCIMB13113). The physiological diversity these two strains was demonstrated by comparative fed-batch cultivation displaying highly different consumption...
Bacillus methanolicus MGA3 is a facultative methylotroph of industrial relevance that able to grow on methanol as its sole source carbon and energy. The Gram-positive bacterium possesses soluble NAD(+) -dependent dehydrogenase assimilates formaldehyde via the ribulose monophosphate (RuMP) cycle. We used label-free quantitative proteomics generate reference proteome data for this compared B. two different sources (methanol mannitol) well growth temperatures (50°C 37°C). From total...
The increasing global demand for food and energy production encourages the development of new strategies focused on sustainability. Often, microbial bioprocesses rely or feed competitive feedstocks; hence, there is a trending need green substrates. Here, we have proven potential brown seaweed biomass as feedstock account its content mannitol glucose polymer laminarin. Our host, Corynebacterium glutamicum, was engineered to enable access carbon source through heterologous expression...
ABSTRACT Polyene macrolide antibiotics, including nystatin and amphotericin B, possess fungicidal activity are being used as antifungal agents to treat both superficial invasive fungal infections. Due their toxicity, however, clinical applications relatively limited, new-generation polyene macrolides with an improved therapeutic index highly desirable. We subjected the polyol region of heptaene analogue S44HP biosynthetic engineering designed remove introduce hydroxyl groups in C-9-C-10...
ABSTRACT Aspartokinase (AK) controls the carbon flow into aspartate pathway for biosynthesis of amino acids l -methionine, -threonine, -isoleucine, and -lysine. We report here cloning four genes ( asd , encoding semialdehyde dehydrogenase; dapA dihydrodipicolinate synthase; dapG AKI; yclM AKIII) in Bacillus methanolicus MGA3. Together with known AKII gene lysC form a set three AK this organism. Overexpression increased -lysine production wild-type B. strain MGA3 2-, 10-, 60-fold...
Establishment of sustainable technology for methanol-based production acetoin by metabolically engineered<italic>Bacillus methanolicus</italic>.
Abstract The growing need of next generation feedstocks for biotechnology spurs an intensification research on the utilization methanol as carbon and energy source biotechnological processes. In this paper, we introduced methanol‐based overproduction riboflavin into metabolically engineered Bacillus methanolicus MGA3. First, showed that B. naturally produces small amounts riboflavin. Then, created strains overexpressing either homologous or heterologous gene clusters encoding biosynthesis...
The polyene macrolide antibiotic nystatin produced by Streptomyces noursei contains a deoxyaminosugar mycosamine moiety attached to the C-19 carbon of macrolactone ring through beta-glycosidic bond. biosynthetic gene cluster three genes, nysDI, nysDII, and nysDIII, encoding enzymes with presumed roles in biosynthesis attachment as glycosyltransferase, aminotransferase, GDP-mannose dehydratase, respectively. In present study, functions these genes were analyzed. recombinant NysDIII protein...
The gram-positive bacterium Bacillus methanolicus MGA3 is a promising candidate for methanol-based biotechnologies. Accurate determination of intracellular metabolites crucial engineering this bacteria into an efficient microbial cell factory. Due to the diversity chemical and properties, experimental protocol validated on B. needed. Here systematic evaluation different techniques establishing reliable basis metabolome investigations presented. Metabolome analysis was focused closely linked...
The use of methanol as carbon source for biotechnological processes has recently attracted great interest due to its relatively low price, high abundance, purity, and the fact that it is a non-food raw material. In this study, methanol-based production 5-aminovalerate (5AVA) was established using recombinant Bacillus methanolicus strains. 5AVA building block polyamides candidate become C5 platform chemical of, among others, δ-valerolactam, 5-hydroxy-valerate, glutarate, 1,5-pentanediol. we...
ABSTRACT We investigated the regulation and roles of six aspartate pathway genes in l -lysine overproduction Bacillus methanolicus : dapG , encoding aspartokinase I (AKI); lysC AKII; yclM AKIII; asd semialdehyde dehydrogenase; dapA dihydrodipicolinate synthase; lysA meso -diaminopimelate decarboxylase. Analysis wild-type strain revealed that vivo transcription was repressed 5-fold by induced 2-fold dl -methionine added to growth medium. Surprisingly, -methionine, while were not significantly...