A5015339759- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Amino Acid Enzymes and Metabolism
- Bacterial Genetics and Biotechnology
- biodegradable polymer synthesis and properties
- GABA and Rice Research
- Enzyme Structure and Function
- Polyamine Metabolism and Applications
- Catalysis for Biomass Conversion
- Microbial Fuel Cells and Bioremediation
- Viral Infectious Diseases and Gene Expression in Insects
- Music and Audio Processing
Bielefeld University
2019-2024
Methanol is a sustainable substrate for biotechnology. In addition to natural methylotrophs, metabolic engineering has gained attention transfer of methylotrophy. Here, we engineered Corynebacterium glutamicum methanol-dependent growth with sugar co-substrate. Heterologous expression genes methanol dehydrogenase from Bacillus methanolicus and ribulose monophosphate pathway hexulose phosphate synthase isomerase subtilis enabled mutants carrying one two independent cut-offs, i.e., either...
Establishment of sustainable technology for methanol-based production acetoin by metabolically engineered<italic>Bacillus methanolicus</italic>.
Bio-based plastics represent an increasing percentage of the economy. The fermentative production bioplastic monomer 5-aminovalerate (5AVA), which can be converted to polyamide 5 (PA 5), has been established in Corynebacterium glutamicum via two metabolic pathways. l-lysine 5AVA by either oxidative decarboxylation and subsequent deamination or cadaverine followed transamination oxidation. Here, a new three-step pathway was using monooxygenase putrescine oxidase (Puo), catalyzes cadaverine,...
Abstract Background Bacillus methanolicus is the next workhorse in biotechnology using methanol, an alternative and economical one-carbon feedstock that can be obtained directly from carbon dioxide, as both energy source for production of various value-added chemicals. The wild-type strain B. MGA3 naturally overproduces l -glutamate methanol-based fed-batch fermentations. Results Here we generated, by induced mutagenesis, evolved exhibiting enhanced capability (>150%). To showcase...
The methylotrophic thermophile Bacillus methanolicus can utilize the non-food substrate methanol as its sole carbon and energy source. Metabolism of L -lysine, in particular biosynthesis, has been studied to some detail, methanol-based -lysine production achieved. However, little is known about degradation, which may proceed via 5-aminovalerate (5AVA), a non-proteinogenic ω-amino acid with applications bioplastics. physiological role 5AVA related compounds native methylotroph was unknown....