A5074559631- Amino Acid Enzymes and Metabolism
- Bacterial Genetics and Biotechnology
- Microbial Metabolic Engineering and Bioproduction
- GABA and Rice Research
- Enzyme Structure and Function
- Polyamine Metabolism and Applications
- Protein Hydrolysis and Bioactive Peptides
- Viral Infectious Diseases and Gene Expression in Insects
- Biofuel production and bioconversion
- Bacteriophages and microbial interactions
- Enzyme Catalysis and Immobilization
- Cancer Research and Treatments
- Probiotics and Fermented Foods
- Protein purification and stability
- RNA and protein synthesis mechanisms
SINTEF
2010-2024
Summary Methanol is regarded as an attractive substrate for biotechnological production of value‐added bulk products, such amino acids and polyamines. In the present study, methylotrophic thermophilic bacterium B acillus methanolicus was engineered into a microbial cell factory platform chemical 1,5‐diaminopentane (cadaverine) from methanol. This achieved by heterologous expression E scherichia coli genes cad A ldc C encoding two different lysine decarboxylase enzymes, increasing overall L...
Bacillus methanolicus is a thermophilic methylotroph able to overproduce amino acids from methanol, substrate not used for human or animal nutrition. Based on our previous RNA-seq analysis mannitol inducible promoter and putative activator gene mtlR were identified. The was applied controlled expression using fluorescent reporter proteins flow cytometry analysis, improved by changing the -35 region co-expression of regulator gene. For independent complementary control, heterologous...
The use of methanol as carbon source for biotechnological processes has recently attracted great interest due to its relatively low price, high abundance, purity, and the fact that it is a non-food raw material. In this study, methanol-based production 5-aminovalerate (5AVA) was established using recombinant Bacillus methanolicus strains. 5AVA building block polyamides candidate become C5 platform chemical of, among others, δ-valerolactam, 5-hydroxy-valerate, glutarate, 1,5-pentanediol. we...
We previously designed the consensus signal peptide (CSP) and demonstrated that it can be used to strongly stimulate heterologous protein production in Escherichia coli. A comparative study using CSP two bacterial sequences, pelB ompA, showed effect of sequences on both expression level translocation efficiency highly specific. report here generation mutant libraries by a combinatorial mutagenesis approach. Degenerated oligonucleotides were cloned frame with 5' end bla gene, encoding mature...
ABSTRACT We investigated the regulation and roles of six aspartate pathway genes in l -lysine overproduction Bacillus methanolicus : dapG , encoding aspartokinase I (AKI); lysC AKII; yclM AKIII; asd semialdehyde dehydrogenase; dapA dihydrodipicolinate synthase; lysA meso -diaminopimelate decarboxylase. Analysis wild-type strain revealed that vivo transcription was repressed 5-fold by induced 2-fold dl -methionine added to growth medium. Surprisingly, -methionine, while were not significantly...
The facultative methylotroph Bacillus methanolicus MGA3 has previously been genetically engineered to overproduce the amino acids L-lysine and L-glutamate their derivatives cadaverine γ-aminobutyric acid (GABA) from methanol at 50°C. We here explored potential of utilizing sugar alcohol mannitol seaweed extract (SWE) containing mannitol, as alternative feedstocks for production chemicals by fermentation using B. methanolicus. Extracts brown algae Saccharina latissima harvested in Trondheim...
Abstract Background Bacillus methanolicus is the next workhorse in biotechnology using methanol, an alternative and economical one-carbon feedstock that can be obtained directly from carbon dioxide, as both energy source for production of various value-added chemicals. The wild-type strain B. MGA3 naturally overproduces l -glutamate methanol-based fed-batch fermentations. Results Here we generated, by induced mutagenesis, evolved exhibiting enhanced capability (>150%). To showcase...