A5076797273- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Probiotics and Fermented Foods
- Enzyme Structure and Function
- Amino Acid Enzymes and Metabolism
- Polyamine Metabolism and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Advanced Biosensing Techniques and Applications
- Bacterial Genetics and Biotechnology
- GABA and Rice Research
- Cancer Research and Treatments
- Microbial Metabolism and Applications
- Metabolism and Genetic Disorders
- Biopolymer Synthesis and Applications
- Glycosylation and Glycoproteins Research
- Bioeconomy and Sustainability Development
- Biochemical Acid Research Studies
- Protein Hydrolysis and Bioactive Peptides
- Protein Structure and Dynamics
- Fault Detection and Control Systems
- Advanced Chemical Sensor Technologies
- Advanced Control Systems Optimization
- Advanced Fluorescence Microscopy Techniques
- Enzyme Production and Characterization
Norwegian University of Science and Technology
2019-2024
Trondheim Kommune
2021
Bielefeld University
2016-2019
Instituto de Biotecnología de León
2016
ABSTRACT Gamma‐aminobutyric acid (GABA) is a non‐protein amino widespread in Nature. Among the various uses of GABA, its lactam form 2‐pyrrolidone can be chemically converted to biodegradable plastic polyamide‐4. In metabolism, GABA synthesized either by decarboxylation l ‐glutamate or pathway that starts with transamination putrescine. Fermentative production from glucose recombinant Corynebacterium glutamicum has been described via both routes. Putrescine‐based was characterized...
Corynebacterium glutamicum is used for the million-ton scale production of amino acids and has recently been engineered cyclic non-proteinogenic acid L-pipecolic (L-PA). In this synthetic pathway L-lysine was converted to L-PA by oxidative deamination, dehydration reduction 6-dehydrogenase (deaminating) from Silicibacter pomeroyi pyrroline 5-carboxylate reductase C. glutamicum. However, occurred as by-product only. Here, author show that abolishing export respective gene deletion resulted in...
The increasing global demand for food and energy production encourages the development of new strategies focused on sustainability. Often, microbial bioprocesses rely or feed competitive feedstocks; hence, there is a trending need green substrates. Here, we have proven potential brown seaweed biomass as feedstock account its content mannitol glucose polymer laminarin. Our host, Corynebacterium glutamicum, was engineered to enable access carbon source through heterologous expression...
The dicarboxylic acid glutarate is an important building-block gaining interest in the chemical and pharmaceutical industry. Here, a synthetic pathway for fermentative production of by actinobacterium Corynebacterium glutamicum has been developed. does not require molecular oxygen operates via lysine decarboyxylase followed two transamination NAD-dependent oxidation reactions. Using genome-streamlined L-lysine producing strain as basis, metabolic engineering was performed to enable...
Pipecolic acid or L-PA is a cyclic amino derived from L-lysine which has gained interest in the recent years within pharmaceutical and chemical industries. can be produced efficiently using recombinant Corynebacterium glutamicum strains by expanding natural biosynthetic pathway. six-membered ring homolog of five-membered L-proline, serves as compatible solute C. glutamicum. Here, we show that de novo synthesized externally added partially beneficial for growth under hyper-osmotic stress...
Bacteriocins are antimicrobial peptides produced by bacteria to inhibit competitors in their natural environments. Some of these have emerged as commercial food preservatives and, due the rapid increase antibiotic resistant bacteria, also discussed interesting alternatives antibiotics for therapeutic purposes. Currently, bacteriocins exclusively with producer organisms on complex substrates and sold semi-purified preparations or crude fermentates. To allow clinical application, efficacy...
Abstract The growing need of next generation feedstocks for biotechnology spurs an intensification research on the utilization methanol as carbon and energy source biotechnological processes. In this paper, we introduced methanol‐based overproduction riboflavin into metabolically engineered Bacillus methanolicus MGA3. First, showed that B. naturally produces small amounts riboflavin. Then, created strains overexpressing either homologous or heterologous gene clusters encoding biosynthesis...
Residual streams from lignocellulosic processes contain sugar mixtures of glucose, xylose, and mannose. Here, the industrial workhorse Corynebacterium glutamicum was explored as a research platform for rational utilization multiple substrate. The endogenous manA gene overexpressed to enhance mannose utilization. overexpression xylA Xanthomonas campestris in combination with xylB enabled xylose consumption by C. glutamicum. Furthermore, riboflavin production triggered overexpressing sigH...
Functionalization of amino acids and their derivatives opens up the possibility to produce novel compounds with additional functional groups, which can expand application spectra. Hydroxylation polyamide building blocks might allow crosslinking between molecular chains by esterification. Consequently, this alter properties resulting polymers. C. glutamicum represents a well-known industrial workhorse has been used extensively lysine derivatives. These are as for chemical pharmaceutical...
Availability of measurements key process variables is great importance to allow real time monitoring and control applications. However, in microbial fermentation processes, the unavailability sensors or their high cost a major barrier automated In this study, we implemented an Extended Kalman Filter (EKF) Unscented (UKF), both augmented take state constraints into account, order estimate biomass formation, sugar consumption CO2 formation fed-batch bacterial cultivation process. The filters...
Abstract Riboflavin overproduction by Corynebacterium glutamicum was achieved screening synthetic operons, enabling fine-tuned expression of the riboflavin biosynthetic genes ribGCAH. The operons were designed means predicted translational initiation rates each open reading frame, with best-performing selection without negatively affecting cell growth. Overexpression fructose-1,6-bisphosphatase ( fbp ) and 5-phosphoribosyl 1-pyrophosphate aminotransferase purF encoding then done to redirect...
Fed-batch bioreactors are multiple input, output non-linear systems with a central role in the production of antimicrobials, fine-chemicals and desirable products pharmaceutical industry, as such industrial microbial bio-catalytic processes require understanding microorganism, bioreactor, set differential equations that allow description fermentation system. In present study, we show our preliminary modelling results growth Corynebacterium glutamicum wild type strain ATCC13032 single sugar...