A5040923213- Genomics and Chromatin Dynamics
- DNA Repair Mechanisms
- Cancer, Hypoxia, and Metabolism
- Inflammatory mediators and NSAID effects
- Eicosanoids and Hypertension Pharmacology
- RNA Research and Splicing
- PARP inhibition in cancer therapy
- Microtubule and mitosis dynamics
- Neuroblastoma Research and Treatments
- Cancer-related Molecular Pathways
- Advanced Fluorescence Microscopy Techniques
- Nuclear Structure and Function
- Sirtuins and Resveratrol in Medicine
- Estrogen and related hormone effects
- Cell death mechanisms and regulation
- Cancer, Stress, Anesthesia, and Immune Response
- Cancer-related gene regulation
- Chemical Synthesis and Analysis
- Cellular Mechanics and Interactions
- Wnt/β-catenin signaling in development and cancer
- RNA Interference and Gene Delivery
- Axon Guidance and Neuronal Signaling
- Genomic variations and chromosomal abnormalities
- Cancer-related molecular mechanisms research
- Cancer therapeutics and mechanisms
University of Cambridge
2009-2019
The Gurdon Institute
2009-2019
At Bristol
2019
University of Bristol
2007-2018
Wellcome Trust
2009
Cancer Research UK
2005-2007
SIRT6 belongs to the sirtuin family of protein lysine deacetylases, which regulate aging and genome stability. We found that human has a role in promoting DNA end resection, crucial step double-strand break (DSB) repair by homologous recombination. depletion impaired accumulation replication A single-stranded at damage sites, reduced rates recombination, sensitized cells DSB-inducing agents. identified DSB resection CtIP [C-terminal binding (CtBP) interacting protein] as interaction partner...
Abstract Cyclooxygenase (COX)-2, the inducible key enzyme for prostanoid biosynthesis, is overexpressed in most colorectal carcinomas and a subset of adenomas. Genetic, biochemical, clinical evidence indicates an important role COX-2 tumorigenesis. Although can be induced by aberrant growth factor signaling oncogene activation during tumorigenesis, microenvironmental factors such as hypoxia regulation remains to elucidated. For first time, we report that under hypoxic conditions protein...
// Gabriella Cunha Vieira 1, * , S. Chockalingam Zsombor Melegh 2 Alexander Greenhough 3 Sally Malik 1 Marianna Szemes Ji Hyun Park Abderrahmane Kaidi Li Zhou 4 Daniel Catchpoole Rhys Morgan David O. Bates 5 Peter Gabb Karim Cancer Epigenetics Laboratory and, School of Cellular & Molecular Medicine, University Bristol, UK Department Pathology, Southmead Hospital, Colorectal Laboratory, The Kids Research Institute, Children's Hospital at Westmead, New South Wales, Australia Biology, Division...
Abstract Increased expression of cyclooxygenase‐2 (COX‐2) and subsequent prostaglandin production is an important event in several human malignancies, including colorectal cancer. COX‐2 mediated prostanoid synthesis has been shown to play a key role tumor progression with E 2 (PGE ) being promote growth, invasion angiogenesis. The the other prostaglandins produced by tumors remains poorly understood. We have that cells produce F α (PGF provide evidence PGF may tumorigenesis. Our data show...
Neuroblastoma is a biologically and clinically heterogeneous pediatric malignancy that includes high-risk subset for which new therapeutic agents are urgently required. As well as MYCN amplification, activating point mutations of ALK NRAS associated with relapsing neuroblastoma. both RAS signal through the MEK/ERK pathway, we sought to evaluate two previously reported inhibitors ETS-related transcription factors, transcriptional mediators Ras-MEK/ERK pathway in other cancers. Here show...
Changes in chromatin structure are key determinants of genomic responses. Thus, methods that enable such measurements instrumental for investigating genome regulation and function. Here, we report further developments validation a streamlined method histone-based fluorescence lifetime imaging microscopy (FLIM) robustly detects compaction states fixed live cells, 2D 3D. We present quality-controlled detailed is simpler faster than previous methods, uses FLIMfit open-source software....
Abstract Changes in chromatin compaction are crucial during genomic responses. Thus, methods that enable such measurements instrumental for investigating genome function. Here, we address this challenge by developing, validating, and streamlining histone-based fluorescence lifetime imaging microscopy (FLIM) robustly detects states fixed live cells; 2D 3D. We present quality-controlled detailed method is simpler faster than previous approches, uses FLIMfit open-source software. demonstrate...