A5036684937- Malaria Research and Control
- Mosquito-borne diseases and control
- Trypanosoma species research and implications
- Toxoplasma gondii Research Studies
- Lipid Membrane Structure and Behavior
- Complement system in diseases
- Parasitic infections in humans and animals
- Parasites and Host Interactions
- Virus-based gene therapy research
- Peptidase Inhibition and Analysis
- Drug Transport and Resistance Mechanisms
- HIV Research and Treatment
- Aquaculture disease management and microbiota
- Apelin-related biomedical research
- Vector-borne infectious diseases
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Viral Infections and Outbreaks Research
- Immune Cell Function and Interaction
- Lysosomal Storage Disorders Research
- Viral gastroenteritis research and epidemiology
- Helminth infection and control
- Parasitic Diseases Research and Treatment
- HIV/AIDS drug development and treatment
- RNA Research and Splicing
- Carbohydrate Chemistry and Synthesis
Philipps University of Marburg
2009-2022
TU Dortmund University
2014
Max Planck Society
2013
Petra Christian University
2006
University of Veterinary Medicine Hannover, Foundation
1997
Specific microRNAs (miRNAs), including miR-134, localize to neuronal dendrites, where they control synaptic protein synthesis and plasticity. However, the mechanism of miRNA transport is unknown. We found that precursor-miRNA-134 (pre-miR-134) accumulates in dendrites hippocampal neurons at synapses vivo. Dendritic localization pre-miR-134 mediated by DEAH-box helicase DHX36, which directly associates with terminal loop. DHX36 function required for miR-134-dependent inhibition target gene...
To form new blood vessels (angiogenesis), endothelial cells (ECs) must be activated and acquire highly migratory proliferative phenotypes. However, the molecular mechanisms that govern these processes are incompletely understood. Here, we show Apelin signaling functions to drive ECs into such an angiogenic state. Zebrafish lacking exhibit defects in tip cell morphology sprouting. Using transplantation experiments, find mosaic vessels, wild-type leave dorsal aorta (DA) while neighboring...
Peptide hormones are key messengers in the signaling network between nervous system, endocrine glands, energy stores and gastrointestinal tract that regulates feeding metabolism. Studies on Drosophila system have uncovered parallels homologies homeostatic peptidergic fruit flies vertebrates. Yet, role of enteroendocrine peptides regulation metabolism has not been explored, with research hampered by unknown identity produced fly's intestinal tract. We performed a peptidomic LC/MS analysis fly...
Background Highly charged compounds typically suffer from low membrane permeability and thus are generally regarded as sub-optimal drug candidates. Nonetheless, the highly fosmidomycin its more active methyl-derivative FR900098 have proven parasiticidal activity against erythrocytic stages of malaria parasite Plasmodium falciparum. Both target isoprenoid biosynthesis pathway present in bacteria plastid-bearing organisms, like apicomplexan parasites. Surprisingly, inactive a range...
ABSTRACT Both apicomplexan parasites Toxoplasma gondii and Plasmodium falciparum lack type I NADH dehydrogenases (complex I) but instead carry alternative (type II) dehydrogenases, which are absent in mammalian cells thus considered promising antimicrobial drug targets. The quinolone-like compound 1-hydroxy-2-dodecyl-4(1 H )quinolone (HDQ) was recently described as a high-affinity inhibitor of fungal enzymatic assays, probably by interfering with the ubiquinol binding site enzyme. We...
Abstract The Down syndrome-associated DYRK1A kinase has been reported as a stimulator of the developmentally important Hedgehog (Hh) pathway, but cells from syndrome patients paradoxically display reduced Hh signalling activity. Here we find that stimulates GLI transcription factor activity through phosphorylation general nuclear localization clusters. In contrast, in vivo and vitro experiments reveal can also function an inhibitor endogenous by negatively regulating ABLIM proteins, actin...
The kinome of the human malaria parasite Plasmodium falciparum comprises representatives most eukaryotic protein kinase groups, including kinases which regulate proliferation and differentiation processes. Despite extensive research on plasmodial enzymes, little information is available regarding four identified members cyclin-dependent kinase-like (CLK) family. In other eukaryotes, CLKs mRNA splicing through phosphorylation Serine/Arginine-rich proteins. Here, we investigate two PfCLKs,...
The largest Ebola virus (EBOV) epidemic in West Africa ever caused more than 28,000 cases and 11,000 deaths, the current EBOV Democratic Republic of Congo continues, with 3,000 to date. Therefore, it is essential develop antivirals against EBOV. Recently, an inhibitor cellular phosphatase PP2A-mediated dephosphorylation transcription factor VP30 has been shown suppress spread virus. Here, we identified protein kinase SRPK1 as a VP30-specific that phosphorylates serine 29, same residue...
The intraerythrocytic developmental stages of the malaria parasite Plasmodium falciparum are responsible for clinical symptoms associated with tropica. non-infected human erythrocyte is a terminally differentiated cell that unable to synthesize proteins and lipids de novo, it incapable importing number solutes essential proliferation. Approximately 12-15 h after invasion parasitized undergoes marked increase in its permeability variety different present extracellular milieu. due induction...
Human Onchocerca volvulus infection sera were found to recognize zwitterionic glycolipids of O. and cross-react with those other parasitic nematodes (Ascaris suum, Setaria digitata Litomosoides sigmodontis). By the use an epitope-specific monoclonal antibody, all these nematode species observed contain antigenic determinant phosphocholine. A hyperimmune serum specific for arthro-series glycolipid structures reacted various neutral nematodes, which demonstrated that their oligosaccharide...
The intraerythrocytic development of P. falciparum induces New Permeability Pathways (NPP) in the membrane parasitized erythrocyte which provide parasite with nutrients, adjust electrolyte composition to needs parasite, and dispose metabolic waste products osmolytes. Patch-clamp recordings identified inwardly outwardly rectifying (OR) anion conductances host as electrophysiological correlate NPP. OR conductance is regulated by serum. Here we show that serum albumin (SA) stimulated...
The presence of glycosphingolipids in the metacestodes fox tapeworm, Taenia crassiceps , has been established. normal‐phase TLC pattern neutral‐fraction glycolipids revealed groups bands corresponding to homologous components increasing sugar chain length. three simplest glycolipid have isolated and their chemical constitution determined as being neogala series: Galβ1Cer, Galβ6Galβ1Cer Galβ6Galβ6Galβ1Cer. ceramide tetrasaccharide fraction found consist a mixture neogalatetraosylceramide, an...
Heavy metals are required by all organisms for normal function, but high levels of heavy toxic. Therefore, homeostasis these is crucial. In the human malaria-causing agent Plasmodium falciparum, mechanisms metal transport have yet to be characterized. We developed a P. falciparum line resistant from wild-type sensitive metals. A molecular and biochemical analysis involvement multidrug resistance 2 (pfmdr2) gene, an ABC-type transporter, in was studied. Using novel uptake assay applied on two...
In addition to the catalytically active kinase domain, most kinases feature regulatory domains that govern their activity. Modulating and interfering with these interdomain interactions presents a major opportunity for understanding biological systems developing novel therapeutics. Therefore, small molecule inhibitors target through an allosteric mode of action have high intrinsic selectivity, as are often unique single or family. Here we report development iFLiK (interface-Fluorescent...
SYNCRIP, a member of the cellular heterogeneous nuclear ribonucleoprotein (hnRNP) family RNA binding proteins, regulates various aspects neuronal development and plasticity. Although SYNCRIP has been identified as component cytoplasmic granules in dendrites mammalian neurons, only little is known about specific target mRNAs that mediate its effect on morphogenesis function. Here, we present comprehensive characterization mRNA interactome using iCLIP primary rat cortical neurons. We identify...
During development in human erythrocytes, Plasmodium falciparum parasites display a remarkable number of adhesive proteins on their plasma membrane. In the invasive merozoites, these include members PfMSP1 and PfAMA1/RON complexes, which facilitate contact between merozoites red blood cells. gametocytes, sexual precursor cells mediating parasite transmission to mosquito vector, membrane-associated primarily belong PfCCp 6-cys families with roles fertilization. This study describes newly...
In its host erythrocyte, the malaria parasite<i>Plasmodium falciparum</i> resides within a parasitophorous vacuole, membrane of which forms barrier between cell cytosol and parasite surface. The vacuole is unique compartment because it contains specific proteins that are believed to be involved in biological functions essential for survival. As prerequisite characterization vacuolar proteome, we have developed an experimental approach allows selective biotinylation soluble proteins. This...