A5050471321- MicroRNA in disease regulation
- RNA Research and Splicing
- Circular RNAs in diseases
- Cellular Mechanics and Interactions
- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced Fluorescence Microscopy Techniques
- Neuroscience and Neuropharmacology Research
- Cell Image Analysis Techniques
- Cancer-related molecular mechanisms research
- Cell Adhesion Molecules Research
- RNA regulation and disease
- Genomics and Chromatin Dynamics
- Fibroblast Growth Factor Research
- Mechanisms of cancer metastasis
- Eosinophilic Esophagitis
- Microtubule and mitosis dynamics
- Asthma and respiratory diseases
- RNA Interference and Gene Delivery
- Receptor Mechanisms and Signaling
- Protein Kinase Regulation and GTPase Signaling
- Obsessive-Compulsive Spectrum Disorders
- Protease and Inhibitor Mechanisms
- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Neurogenesis and neuroplasticity mechanisms
Philipps University of Marburg
2013-2025
Justus-Liebig-Universität Gießen
2021-2025
University Hospital Heidelberg
2009-2011
Heidelberg University
2009-2011
Specific microRNAs (miRNAs), including miR-134, localize to neuronal dendrites, where they control synaptic protein synthesis and plasticity. However, the mechanism of miRNA transport is unknown. We found that precursor-miRNA-134 (pre-miR-134) accumulates in dendrites hippocampal neurons at synapses vivo. Dendritic localization pre-miR-134 mediated by DEAH-box helicase DHX36, which directly associates with terminal loop. DHX36 function required for miR-134-dependent inhibition target gene...
MicroRNAs (miRNAs) are small non-coding RNAs with important functions in the development and plasticity of post-mitotic neurons. In addition to well-described cytoplasmic function miRNAs post-transcriptional gene regulation, recent studies suggested that could also be involved transcriptional regulatory processes nuclei proliferating cells. However, whether localize within nucleus neurons is unknown. Using a combination microarray hybridization RNA deep sequencing, we identified specific...
Abstract Bipolar disorder (BD) is a severe and highly heritable neuropsychiatric with lifetime prevalence of 1%. Molecular genetic studies have identified the first BD susceptibility genes. However, disease pathways remain largely unknown. Accumulating evidence suggests that microRNAs, class small noncoding RNAs, contribute to basic mechanisms underlying brain development plasticity, suggesting their possible involvement in pathogenesis several psychiatric disorders, including BD. In present...
Neuronal activity controls the correct establishment and refinement of neuronal circuits by regulating key aspects such as dendritogenesis spine development. Both transcriptional post-transcriptional gene expression programs induced have to be coordinated in a tight spatio-temporal manner order for proper functioning neuron. In this context microRNAs (miRNAs), which are implicated regulation, good candidates control dendritic recent study we demonstrated that induces myocyte enhancing factor...
Studies of the past decade established cyclase-associated protein (CAP) as a key regulator actin dynamics and associated its dysregulation with human brain disorders. However, neuronal functions remained unknown until recent studies deciphered CAP-dependent mechanisms relevant for neuron differentiation or synapse morphogenesis, which are summarized discussed in this forum article.
MicroRNAs are important regulators of local protein synthesis during neuronal development.We investigated the dynamic regulation microRNA production and found that majority microRNA-generating complex, consisting Dicer, TRBP, PACT, specifically associates with intracellular membranes in developing neurons.Stimulation brain-derived neurotrophic factor (BDNF), which promotes dendritogenesis, caused redistribution TRBP from endoplasmic reticulum into cytoplasm, its dissociation a Ca 2+...
Cyclase-associated proteins (CAPs) are evolutionary-conserved actin-binding with crucial functions in regulating actin dynamics, the spatiotemporally controlled assembly and disassembly of filaments (F-actin). Mammals possess two family members (CAP1 CAP2) different expression patterns. Unlike most other tissues, both CAPs expressed brain present hippocampal neurons. We recently reported roles for CAP1 growth cone function, neuron differentiation, connectivity mouse brain. Instead, CAP2...
Abstract The vast majority of excitatory synapses are formed on small dendritic protrusions termed spines. Dendritic spines vary in size and density that crucial determinants synaptic transmission. Aberrations spine morphogenesis can compromise brain function have been associated with neuropsychiatric disorders. Actin filaments (F-actin) the major structural component spines, therefore, actin-binding proteins (ABP) control F-actin dis-/assembly moved into focus as critical regulators...
SYNCRIP, a member of the cellular heterogeneous nuclear ribonucleoprotein (hnRNP) family RNA binding proteins, regulates various aspects neuronal development and plasticity. Although SYNCRIP has been identified as component cytoplasmic granules in dendrites mammalian neurons, only little is known about specific target mRNAs that mediate its effect on morphogenesis function. Here, we present comprehensive characterization mRNA interactome using iCLIP primary rat cortical neurons. We identify...
Serum response factor (SRF) is an essential transcription for brain development and function. Here, we explored how SRF cofactor, the actin monomer-sensing myocardin-related MRTF, regulated in mouse cortical neurons. We found that MRTF-dependent activity vitro vivo was repressed by cyclase-associated protein CAP1. Inactivation of actin-binding CAP1 reduced amount monomers cytoplasm, which promoted nuclear MRTF translocation MRTF-SRF activation. This function independent cofilin1...
The morphology of dendritic spines, the postsynaptic compartment most excitatory synapses, decisively modulates function neuronal circuits as also evident from human brain disorders associated with altered spine density or morphology. Actin filaments (F-actin) form backbone and a number actin-binding proteins (ABP) have been implicated in shaping cytoskeleton mature spines. Instead, only little is known about mechanisms that control reorganization unbranched F-actin immature spines to...
MicroRNAs (miRNAs) are short (∼22 nt) single-stranded non-coding RNAs that regulate gene expression at the post-transcriptional level. Over recent years, many studies have extensively characterized involvement of miRNA-mediated regulation in neurogenesis and brain development. However, a comprehensive catalog cortical miRNAs expressed cell-specific manner progenitor types developing mammalian cortex is still missing. Overcoming this limitation, here we exploited double reporter mouse line...
Recent studies identified cyclase-associated proteins (CAPs) as important regulators of actin dynamics that control assembly and disassembly filaments (F-actin). While these significantly advanced our knowledge their molecular functions, the physiological relevance CAPs largely remained elusive. Gene targeting in mice implicated CAP2 heart physiology skeletal muscle development. Heart defects mutant were associated with altered activity serum response factor (SRF), a transcription involved...
Serum response factor (SRF) is a ubiquitously expressed transcription essential for brain development and function. SRF activity controlled by two competing classes of coactivators, myocardin-related factors (MRTF) ternary complex (TCF), which introduce specificity into gene expression programs. To date, only few studies investigated upstream regulatory mechanisms, mainly focused on TCF. Since an inhibitory function monomeric actin towards MRTF-SRF signaling well-established, we hypothesized...
Abstract The vast majority of excitatory synapses are formed on small dendritic protrusions termed spines. Dendritic spines vary in size and density that crucial determinants synaptic transmission. Aberrations spine morphogenesis can compromise brain function have been associated with neuropsychiatric disorders. Actin filaments (F-actin) the major structural component therefore actin-binding proteins (ABP) control F-actin dis-/assembly moved into focus as critical regulators function....
ABSTRACT MicroRNAs (miRNAs) are short (∼22 nt) single-stranded non-coding RNAs that regulate gene expression at the post-transcriptional level. Over past years, many studies have extensively characterized involvement of miRNA-mediated regulation in neurogenesis and brain development. However, a comprehensive catalog cortical miRNAs cell-specifically expressed progenitor types developing mammalian cortex is still missing. Overcoming this limitation, here we exploited double reporter mouse...