A5101812050- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- RNA regulation and disease
- Advanced biosensing and bioanalysis techniques
- interferon and immune responses
- Hematological disorders and diagnostics
- Biochemical and Molecular Research
- Endoplasmic Reticulum Stress and Disease
- Prenatal Screening and Diagnostics
- Historical and Literary Studies
- Parasitic Infections and Diagnostics
- Philosophy and History of Science
- Edible Oils Quality and Analysis
- Immune Cell Function and Interaction
- Philosophy, Science, and History
- Neurobiology and Insect Physiology Research
- German Literature and Culture Studies
- Classical Studies and Legal History
- Helminth infection and control
- Virology and Viral Diseases
Max Perutz Labs
2016-2025
Medical University of Vienna
2016-2025
Vienna Biocenter
2016-2023
Institut de Microelectrònica de Barcelona
2023
Instituto de Medicina Tropical “Pedro Kourí”
2018
Austrian Academy of Sciences
2006-2016
Institute of Molecular Biotechnology
2006-2016
Saarland University
2008
Universidad de Extremadura
2006
Rockefeller University
2003-2004
microRNAs (miRNAs) are endogenous, noncoding ∼22-nucleotide RNA molecules that have recently emerged as fundamental, post-transcriptional regulators of cognate target gene expression. Many mammalian miRNAs expressed in a tissue-specific manner, phenomenon has so far been attributed to transcriptional regulation. We here show by Northern blots and situ hybridization experiments the expression can be regulated at level. In particular, miR-138 is spatially restricted distinct cell types, while...
Gene silencing in the process of RNA interference is mediated by a ribonucleoprotein complex referred to as RNA-induced (RISC). Here we describe molecular mechanism target cleavage using affinity-purified minimal RISC from human cells. Cleavage proceeds via hydrolysis and release 3′-hydroxyl 5′-phosphate terminus. Substitution 2′-hydroxyl group at site 2′-deoxy had no significant effect, suggesting that product and/or conformational transition rather than chemical step rate-limiting....
Splicing of mammalian precursor transfer RNA (tRNA) molecules involves two enzymatic steps. First, intron removal by the tRNA splicing endonuclease generates separate 5' and 3' exons. In animals, second step predominantly entails direct exon ligation an elusive ligase. Using activity-guided purification ligase from HeLa cell extracts, we identified HSPC117, a member UPF0027 (RtcB) family, as essential subunit complex. interference-mediated depletion HSPC117 inhibited maturation...
Abstract The unfolded protein response ( UPR ) is a conserved stress‐signaling pathway activated after accumulation of proteins within the endoplasmic reticulum ER ). Active signaling leads to unconventional, enzymatic splicing XBP1 mRNA enabling expression transcription factor XBP1s control homeostasis. While IRE1 has been identified as endoribonuclease required for cleavage this mRNA, corresponding ligase in mammalian cells remained elusive. Here, we report that RTCB , catalytic subunit...
Highlights•A third of the RBPome remodels upon SARS-CoV-2 infection•Viral RNPs include 139 cellular and 6 viral RBPs•Inhibition these RBPs hampers infection•The tRNA ligase complex is a key regulator SARS-CoV-2SummarySevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes disease 2019 (COVID-19). relies on RNA-binding proteins (RBPs) to replicate spread, although which control its life cycle remains largely unknown. Here, we employ multi-omic approach identify systematically...
Genetic networks are characterized by extensive buffering. During tumor evolution, disruption of functional redundancies can create de novo vulnerabilities that specific to cancer cells. Here, we systematically search for cancer-relevant paralog interactions using CRISPR screens and publicly available loss-of-function datasets. Our analysis reveals >2,000 candidate dependencies, several which validate experimentally, including CSTF2-CSTF2T, DNAJC15-DNAJC19, FAM50A-FAM50B, RPP25-RPP25L. We...
When Escherichia coli encounters stress, the endoribonuclease MazF initiates a post-transcriptional response that results in reprogramming of protein synthesis. By removing 3΄-terminus 16S rRNA, generates specialized ribosomes selectively translate mRNAs likewise processed by MazF. Given energy required for de novo ribosome biosynthesis, we considered existence repair mechanism operating upon stress relief to recycle modified ribosomes. Here, show stress-ribosomes and 3΄-terminal rRNA...
Polymerase δ is essential for eukaryotic genome duplication and synthesizes DNA at both the leading lagging strands. The polymerase complex a heterotetramer comprising catalytic subunit POLD1 accessory subunits POLD2, POLD3, POLD4. Beyond replication, has emerged as central element in maintenance. essentiality of constrained generation δ–knockout cell lines or model organisms and, therefore, understanding complexity its activity function subunits. To our knowledge, no germline biallelic...