A5016479584- Toxoplasma gondii Research Studies
- Parasitic Infections and Diagnostics
- Herpesvirus Infections and Treatments
- Clostridium difficile and Clostridium perfringens research
- Rabies epidemiology and control
- Salmonella and Campylobacter epidemiology
- Cytomegalovirus and herpesvirus research
- Carbohydrate Chemistry and Synthesis
- Helicobacter pylori-related gastroenterology studies
- Antibiotic Resistance in Bacteria
- Heme Oxygenase-1 and Carbon Monoxide
- Coccidia and coccidiosis research
- Lysosomal Storage Disorders Research
- Gut microbiota and health
- Biochemical and Molecular Research
- Bacteriophages and microbial interactions
- Viral gastroenteritis research and epidemiology
- Polyamine Metabolism and Applications
- Escherichia coli research studies
- Microscopic Colitis
- Insect symbiosis and bacterial influences
- SARS-CoV-2 and COVID-19 Research
- Trace Elements in Health
- Vibrio bacteria research studies
- Pneumocystis jirovecii pneumonia detection and treatment
Universitätsmedizin Göttingen
2016-2025
University of Göttingen
2006-2025
Intelligent Transport Systems Niedersachsen
2024
Otto-von-Guericke University Magdeburg
2023
Nephrologisches Zentrum Goettingen
2019
Institute of Medical Microbiology and Hygiene
1993-2014
University of Würzburg
1993-2003
University of Pennsylvania
1997-1998
South University
1997
Clostridioides difficile infections (CDI) have emerged over the past decade causing symptoms that range from mild, antibiotic-associated diarrhea (AAD) to life-threatening toxic megacolon. In this study, we describe a multiple and isochronal (mixed) CDI caused by isolates DSM 27638, 27639 27640 already initially showed different morphotypes on solid media. The three belonging ribotypes (RT) 012 (DSM 27639) 027 27638 27640) were phenotypically characterized high quality closed genome...
Stage conversion between tachyzoites and bradyzoites of Toxoplasma gondii was investigated in vitro by using murine bone marrow-derived macrophages (BMMs) as host cells. Following infection untreated BMMs with tachyzoites, spontaneous expression bradyzoite-specific antigens (Bsa) occurred at low frequency strain-dependent ratios from 0.03 to 2%. As previously described for peritoneal macrophages, activation tachyzoite-infected gamma interferon (IFN-gamma) or lipopolysaccharide resulted the...
Summary Stage conversion between the tachyzoite and bradyzoite forms of protozoan parasite Toxoplasma gondii is an important aspect in pathogenesis toxoplasmosis. In initial investigation molecular regulation stage T. , we describe cloning characterization a bradyzoite‐specifically expressed gene (hsp30/bag1). Bradyzoite formation was induced cell culture by alkaline pH, this followed purification parasitic using magnetic sorting. A cDNA library constructed random amplification polymerase...
By using stage-specific monoclonal antibodies, an in vitro model has been developed to analyze the kinetics of expression antigens during conversion process between tachyzoites and bradyzoites Toxoplasma gondii. Following infection murine macrophages with bradyzoites, bradyzoite-specific declined, whereas tachyzoite-specific increased first 72 h postinfection. Conversely, gamma interferon-treated infected tachyzoites, inhibitory effect interferon on replication parasites was accompanied by...
ABSTRACT Many apicomplexan parasites, such as Toxoplasma gondii and Plasmodium species, possess a nonphotosynthetic plastid, referred to the apicoplast, which is essential for parasites’ viability displays characteristics similar those of nongreen plastids in plants. In this study, we localized several key enzymes carbohydrate metabolism T. either apicoplast or cytosol by engineering parasites express epitope-tagged fusion proteins. The contains complete set glycolysis, should enable...
ABSTRACT Tachyzoites (VEG strain) that emerge from host cells infected with Toxoplasma gondii sporozoites proliferate relatively fast and double their number every 6 h. This rate of growth is intrinsic, as neither the invaded nor cell type appears to influence emergent tachyzoite replication. Fast was not persistent, following ∼20 divisions, population uniformly shifted slower growth. Parasites 10 days post-sporozoite infection doubled only once 15 h and, unlike tachyzoites, they grew at...
ABSTRACT Both apicomplexan parasites Toxoplasma gondii and Plasmodium falciparum lack type I NADH dehydrogenases (complex I) but instead carry alternative (type II) dehydrogenases, which are absent in mammalian cells thus considered promising antimicrobial drug targets. The quinolone-like compound 1-hydroxy-2-dodecyl-4(1 H )quinolone (HDQ) was recently described as a high-affinity inhibitor of fungal enzymatic assays, probably by interfering with the ubiquinol binding site enzyme. We...
ABSTRACT Microsporidia are intracellular organisms of increasing importance as opportunistic pathogens in immunocompromised patients. Host cells infected by the extrusion and injection polar tubes located within spores. The spore is surrounded a rigid wall which, addition to providing mechanical resistance, might be involved host cell recognition initiation infection process. A 51-kDa outer protein was identified Encephalitozoon cuniculi with aid monoclonal antibody, corresponding gene, SWP1...
The identification and characterization of clinical Clostridioides difficile isolates with reduced fidaxomicin susceptibility.Agar dilution assays were used to determine MICs. Genome sequence data obtained by single-molecule real-time (SMRT) sequencing in addition amplicon rpoB rpoC alleles. Allelic exchange was introduce the identified mutation into C. 630Δerm. Replication rates, toxin A/B production spore formation determined from strain susceptibility.Out 50 isolates, isolate Goe-91...
The establishment of culture conditions suitable for inducing differentiation Toxoplasma gondii tachyzoites into parasites resembling the latent bradyzoite form has opened this important developmental transition to experimental analysis. In order develop a genetic marker positive and negative selection during parasite differentiation, T. HXGPRT gene was placed under control 5′ flanking sequences derived from two bradyzoite-specific genes: BAG1 LDH2. Random transgene integration at undefined...
ABSTRACT The apicomplexan parasite Toxoplasma gondii expresses type II NADH dehydrogenases (NDH2s) instead of canonical complex I at the inner mitochondrial membrane. These non-proton-pumping enzymes are considered to be promising drug targets due their absence in mammalian cells. We recently showed by inhibition kinetics that T. NDH2-I is a target quinolone-like compound 1-hydroxy-2-dodecyl-4(1 H )quinolone (HDQ), which inhibits replication nanomolar range. In this study, cationic...
In many apicomplexan parasites the entry of electrons from NADH into electron transport chain is governed by type II dehydrogenases (NDH2s) instead a canonical complex I. Toxoplasma gondii expresses two NDH2 isoforms, TgNDH2-I and TgNDH2-II with no indication for stage-specific regulation. We dissected orientation both isoforms using split GFP assay protease protection after selective membrane permeabilization. The approaches revealed that TgNDH2 are internal enzymes facing their active...
This study was performed as a head-to-head comparison of the performance characteristics (1) two SARS-CoV-2-specific rapid antigen assays with real-time PCR gold standard well (2) fully automated high-throughput transcription-mediated amplification (TMA) assay and in latent class analysis-based test without several hundred samples low prevalence "real world" setting. Recorded sensitivity specificity NADAL LumiraDx Hologic Aptima SARS-CoV-2 TMA were 0.1429 (0.0194, 0.5835), 0.7644 (0.7016,...
We previously reported the in vitro analysis of stage differentiation Toxoplasma gondii murine bone marrow-derived macrophages. The purpose this study was to generate monoclonal rat antibodies that might be suitable for investigating tachyzoite-bradyzoite interconversion vivo with model. Immunization Fischer rats cysts T. NTE resulted generation seven immunoglobulin G2a, G2b, or M isotype, which were further characterized by immunoblot technique, immunofluorescence assay, immunohistology,...
Plasmid vectors that incorporate sequence elements from the dehydrofolate reductase–thymidylate synthase (DHFR-TS) locus ofToxoplasma gondiiintegrate into parasite genome with remarkably high frequency (>1% of transfected parasites). These may—but need not—include mutantDHFR–TSalleles confer pyrimethamine resistance to transgenic parsites. Large genomic constructs integrate at endogenous by homologous recombination, but cDNA-derived sequences lacking long stretches contiguous DNA (due intron...
Carbapenemase-producing Klebsiella pneumoniae pose an increasing risk for healthcare facilities worldwide. A continuous monitoring of ST distribution and its association with resistance virulence genes is required early detection successful K. lineages. In this study, we used WGS to characterize MDR blaOXA-48-positive isolated from inpatients at the University Medical Center Göttingen, Germany, between March 2013 August 2014. Closed genomes 16 isolates carbapenemase-producing were generated...
Clostridioides (Clostridium) difficile infections (CDI) are considered worldwide as emerging health threat. Uptake of C. spores may result in asymptomatic carrier status or lead to CDI that could range from mild diarrhea, eventually developing into pseudomembranous colitis up a toxic megacolon often results high mortality. Most epidemiological studies date have been performed middle- and income countries. Beside others, the use antibiotics composition microbiome identified major risk factors...
ABSTRACT The invasive stages of apicomplexan parasites enter their host cells through mechanisms which are largely conserved throughout the phylum. Host cell invasion is divided into two distinct events, namely, adhesion onto surface and actual entry process. former mediated microneme proteins secreted at onset establishing contact with surface. Many identified so far contain adhesive domains. We here present genomic corresponding cDNA sequences coding for a 460-amino-acid (aa) protein in...