A5070793093- Pluripotent Stem Cells Research
- Reproductive Biology and Fertility
- CRISPR and Genetic Engineering
- Retinal Diseases and Treatments
- Animal Genetics and Reproduction
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Retinal and Optic Conditions
- Renal and related cancers
- Genomics and Chromatin Dynamics
- Nuclear Structure and Function
- RNA Interference and Gene Delivery
- Retinal Imaging and Analysis
- RNA modifications and cancer
- Glaucoma and retinal disorders
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cellular Mechanics and Interactions
- Nitric Oxide and Endothelin Effects
- Atherosclerosis and Cardiovascular Diseases
- Cancer-related gene regulation
- Tissue Engineering and Regenerative Medicine
- Microtubule and mitosis dynamics
- Neuroinflammation and Neurodegeneration Mechanisms
- Sperm and Testicular Function
- Cancer-related molecular mechanisms research
Kindai University
2017-2024
Cedars-Sinai Smidt Heart Institute
2022
Cedars-Sinai Medical Center
2022
The Gurdon Institute
2010-2018
University of Cambridge
2010-2018
Wellcome Trust
2010-2018
Bio-oriented Technology Research Advancement Institution
2017
Kagoshima University
2016
Tokushima Bunri University
2015
Weatherford College
2013
Amphibian oocytes can rapidly and efficiently reprogram the transcription of transplanted somatic nuclei. To explore factors mechanisms involved, we focused on nuclear actin, an especially abundant component oocyte's nucleus (the germinal vesicle). The existence significance actin has long been debated. Here, found that polymerization plays essential part in transcriptional reactivation pluripotency gene Oct4 (also known as Pou5f1 ). We also signaling protein, Toca-1 , enhances by regulating...
Previous studies have revealed that miR-26a-5p and miR-26b-5p act as tumour suppressors in various types of cancer tissues. Here, we aimed to investigate the functional roles these miRNAs identify their regulatory targets bladder (BC). We performed assays BC cells using transfection mature microRNAs (miRNAs). In silico luciferase reporter analyses were applied target genes miRNAs. The overall survival (OS) patients with was evaluated by Kaplan–Meier method. significantly downregulated...
For a long time, it has been assumed that the only role of sperm at fertilization is to introduce male genome into egg. Recently, ideas have emerged epigenetic state nucleus could influence transcription in embryo. However, conflicting reports challenged existence marks on genes, and there are no functional tests supporting marking embryonic gene expression. Here, we show epigenetically programmed regulate By comparing development sperm- spermatid-derived frog embryos, programming for...
Actin is well known for its cytoskeletal functions, where it helps to control and maintain cell shape architecture, as regulating migration intracellular cargo transport, among others. However, actin also prevalent in the nucleus, genome-regulating roles have been described, including being part of chromatin-remodeling complexes. More recently, with help advances microscopy techniques specialized imaging probes, direct visualization nuclear filament dynamics has helped elucidate new actin,...
To study the correlation between integrity of photoreceptor layer after resolution macular oedema (MO) associated with branch retinal vein occlusion (BRVO) and final visual acuity (VA), to determine prognostic factors for outcome.We retrospectively studied 46 eyes from patients resolved MO secondary BRVO, foveal thickness which was less than 250 microm at observation. We assessed status third high reflectance band (HRB) in fovea using optical coherence tomography (OCT) observation, OCT...
Eggs and oocytes have a remarkable ability to induce transcription of sperm after normal fertilization in somatic nuclei cell nuclear transfer. This eggs is essential for development. Nuclear actin actin-binding proteins been shown contribute transcription, although their mode action elusive. Here, we find that Xenopus Wave1, previously characterized as protein involved cytoskeleton organization, present the oocyte nucleus required efficient transcriptional reprogramming. Moreover, Wave1...
Abstract It is known that differentiated cells can be reprogrammed to an undifferentiated state in oocyte cytoplasm after nuclear transfer. Recently, some reports suggested Xenopus egg extracts have the ability reprogram mammalian somatic cells. Reprogramming events of extract treatment and cell culture extract‐treated not been elucidated. In this experiment, we examined reprogramming reversibly permeabilized or nonpermeabilized porcine fibroblast treatment. The egg‐specific histone B4 was...
Nuclear transfer has been regarded as the only reliable tool for studying nuclear reprogramming of mammalian somatic cells by oocytes. However, is not well suited biochemical analyses molecular mechanisms reprogramming. A cell-free system from oocytes an attractive alternative way to mimic in vitro, since a large number can be treated and analyzed. Nevertheless, using developed mammals. Here, cell extracts porcine were prepared their ability induce was evaluated. Extracts metaphase II (MII)...
After fertilization, sperm and oocyte nuclei are rapidly remodeled to form swollen pronuclei (PN) in mammalian zygotes, the proper formation function of PN key producing totipotent zygotes. However, how mature formed has been unclear. We find that filamentous actin (F-actin) assembles mouse zygotes is required for fully functional PN. The perturbation nuclear dynamics results misregulation genes related genome integrity abnormal development embryos. show F-actin ensures DNA damage repair,...
Full-grown Xenopus oocytes in first meiotic prophase contain an immensely enlarged nucleus, the Germinal Vesicle (GV), that can be injected with several hundred somatic cell nuclei. When nuclei of mammalian cells or cultured lines are into a GV, wide range genes not transcribed donor cells, including pluripotency genes, start to transcriptionally activated, and synthesize primary transcripts continuously for days. Because large size abundance laevis oocytes, this experimental system offers...
Understanding the mechanism of resistance genes to reactivation will help improve success nuclear reprogramming. Using mouse embryonic fibroblast nuclei with normal or reduced DNA methylation in combination chromatin modifiers able erase H3K9me3, H3K27me3, and H2AK119ub1 from transplanted nuclei, we reveal basis for transcriptional reprogramming by oocyte factors. A majority is affected more than one type treatment, suggesting that can require repression through multiple epigenetic...
Oocytes have a remarkable ability to reactivate silenced genes in somatic cells. However, it is not clear how the chromatin architecture of cells affects this transcriptional reprogramming. Here, we investigated relationship between opening and activation. We reveal changes accessibility their relevance reprogramming after transplantation nuclei into Xenopus oocytes. Genes that are silenced, but pre-existing open transcription start sites donor cells, prone be activated nuclear transfer,...
Nuclear transfer to oocytes is an efficient way transcriptionally reprogram somatic nuclei, but its mechanisms remain unclear. Here, we identify a sequence of molecular events that leads rapid transcriptional reprogramming nuclei after transplantation Xenopus oocytes. RNA-seq analyses reveal by results in selective switch transcription toward oocyte rather than pluripotent type, without requiring new protein synthesis. Time-course at the single-nucleus level show induced most transplanted...
Abstract Multicellular organisms consist of multiple cell types. The identity these cells is primarily maintained by cell-type-specific gene expression programs; however, mechanisms that suppress programs are poorly defined. Here we show serum response factor (Srf), a transcription activated various extracellular stimuli, can repress genes and promote cellular reprogramming to pluripotency. Manipulations decrease β-actin monomer quantity result in the nuclear accumulation Mkl1 activation...
Abstract The 28,000-year-old remains of a woolly mammoth, named ‘Yuka’, were found in Siberian permafrost. Here we recovered the less-damaged nucleus-like structures from and visualised their dynamics living mouse oocytes after nuclear transfer. Proteomic analyses demonstrated presence components remains. Nucleus-like tissue homogenate histone- lamin-positive by immunostaining. In reconstructed oocytes, mammoth nuclei showed spindle assembly, histone incorporation partial formation; however,...