A5029388347- Reproductive Biology and Fertility
- Genetic and phenotypic traits in livestock
- Reproductive System and Pregnancy
- Reproductive Physiology in Livestock
- Sperm and Testicular Function
- Genetic Mapping and Diversity in Plants and Animals
- Sirtuins and Resveratrol in Medicine
- Cancer-related molecular mechanisms research
- Genomics and Phylogenetic Studies
- Genetic diversity and population structure
- Neurobiology and Insect Physiology Research
- Pluripotent Stem Cells Research
- Birth, Development, and Health
- Preterm Birth and Chorioamnionitis
- Aquatic life and conservation
- Molecular Biology Techniques and Applications
- Animal Nutrition and Physiology
- Renal and related cancers
- Tissue Engineering and Regenerative Medicine
- Lepidoptera: Biology and Taxonomy
- Kruppel-like factors research
- Animal Genetics and Reproduction
- Pregnancy and preeclampsia studies
- Animal Behavior and Reproduction
- Plant and animal studies
Tokyo University of Agriculture
2014-2023
Thermo Fisher Scientific (Japan)
2019
Reconstituting gametogenesis in vitro is a key goal for reproductive biology and regenerative medicine. Successful reconstitution of primordial germ cells spermatogenesis has recently had significant effect the field. However, recapitulation oogenesis remains unachieved. Here we demonstrate first reconstitution, to our knowledge, entire process mammalian from cells, using an estrogen-receptor antagonist that promotes normal follicle formation, which turn crucial supporting oocyte growth. The...
Significance Human color vision is tri-chromatic, with three opsins expressed in cone photoreceptors that are sensitive the red, green, and blue region of spectrum. As theories predict, such tri- or tetra-chromacy four opsin genes common among mammals, birds, other animals, including insects. However, we discovered dragonflies possess as many 15–33 have evolved through dynamic gene multiplications losses within lineage dragonflies. These differentially between adult larva, well dorsal...
The aim of the present study was to address effect resveratrol-mediated upregulation sirtuin 1 (SIRT1) during oocyte maturation on mitochondrial function, developmental ability oocytes and mechanisms responsible for blockage polyspermic fertilization. Oocytes collected from slaughterhouse-derived ovaries were cultured in TCM-199 medium supplemented with 10% FCS 0 or 20 µM resveratrol (Res). We examined Res SIRT1 expression vitro-matured (Exp 1); fertilization 2); DNA copy number (Mt number),...
Mitochondrial numbers increase during oocyte growth. In this study, we collected oocytes and granulosa cell complexes (OGCs) from early antral follicles (EAFs) of aged cows (> 120 months age) examined the effects resveratrol on mitochondrial generation, degradation, quality in grown vitro. We also gene expression cells. Resveratrol (20 µM) enhanced SIRT1 induced autophagy both cells derived cows. Culturing OGCs with increased DNA copy Furthermore, ATP content improved developmental ability...
Abstract Background Because the Japanese native cattle Kuchinoshima-Ushi have been isolated in a small island and their lineage has intensely protected, it assumed to date that numerous valuable genomic variations are conserved this breed. Results In study, we evaluated genetic features of breed, including single nucleotide polymorphism (SNP) information, by whole-genome sequencing using Genome Analyzer II. A total 64.2 Gb sequence was generated, which 86% obtained reads were successfully...
Follicle development is accompanied by proliferation of granulosa cells and increasing oocyte size. To obtain high-quality oocytes in vitro, it important to understand the processes that occur during follicle differences between vivo vitro development. In present study, were collected from early antral follicles (EAFs, 0.5–0.7 mm diameter), small (SAFs, 1–3 large (LAFs, 3–7 grown oocyte-and-granulosa cell complexes (OGCs), which cultured for 14 days after collection EAFs. Gene expression was...
Cognitive performance in people varies according to time-of-day, with memory retrieval declining the late afternoon-early evening. However, functional roles of local brain circadian clocks remains unclear. Here, we show that hippocampal clock controlled by circadian-dependent transcription factor BMAL1 regulates time-of-day profile. Inducible transgenic dominant negative (dnBMAL1) expression mouse forebrain or hippocampus disrupted memories at Zeitgeber Time 8-12, independently retention...
In general, maternal age affects the quality of oocytes and embryos. The present study aimed to examine features age-associated gene expression profiles bovine embryos as well discover possible countermeasures against events. Comprehensive assays germinal vesicle metaphase II (MII)-stage 8- 16-cell-stage were conducted using next-generation sequencing technology. aged cows showed high genes related oxidative phosphorylation, eIF4 p70S6K signaling, mitochondrial dysfunction in MII-stage...
Insects comprise over half of the described species, and acquisition metamorphosis must have contributed to their diversity prosperity. The order Odonata (dragonflies damselflies) is among most-ancestral insects with drastic morphological changes upon metamorphosis, in which understanding molecular mechanisms will provide insight into evolution incomplete complete insects. In identify metamorphosis-related genes Odonata, we performed comprehensive RNA-sequencing blue-tailed damselfly...
Abstract Maternal immune activation (MIA) contributes to behavioral abnormalities relevant schizophrenia in adult offspring, although the molecular mechanisms underlying MIA-induced changes remain unclear. Here we demonstrated that dietary intake of glucoraphanin (GF), precursor a natural antioxidant sulforaphane, during juvenile and adolescent stages prevented cognitive deficits loss parvalbumin (PV) immunoreactivity medial prefrontal cortex (mPFC) offspring after MIA. Gene set enrichment...
More than 2,500 breeds of chicken are reared throughout the world as a source eggs or meat and pets. The primary ancestor present domestic is widely believed to be red junglefowl, although genetic contributions from other junglefowls cannot excluded entirely. reference genome for was obtained purity which has been debated. There is, at present, insufficient data resolve these interesting issues. In this study, we performed whole-genome sequencing compare various species chicken, including...
Abstract The 28,000-year-old remains of a woolly mammoth, named ‘Yuka’, were found in Siberian permafrost. Here we recovered the less-damaged nucleus-like structures from and visualised their dynamics living mouse oocytes after nuclear transfer. Proteomic analyses demonstrated presence components remains. Nucleus-like tissue homogenate histone- lamin-positive by immunostaining. In reconstructed oocytes, mammoth nuclei showed spindle assembly, histone incorporation partial formation; however,...
While the study of phenotypic variation is a central theme in evolutionary biology, genetic approaches available to understanding this are usually limited because lack genomic information non-model organisms. This explored utility next-generation sequencing (NGS) technologies for studying variations between 2 populations species, Hokkai shrimp (Pandalus latirostris; Decapoda, Pandalidae). Before we performed transcriptome analyses using NGS, examined and differentiation populations. Analyses...
Antrum formation and estradiol (E(2)) secretion are specific features of oocyte granulosa cell complexes (OGCs). This study investigates the effect E(2) on in vitro development bovine OGCs derived from early antral follicles as well expression genes cells (GCs). The supplementation culture medium with either or androstenedione (A(4)) improved nuclear maturation enclosed oocytes. When were cultured containing A(4), developmentally competent secreted more than that not competent. In addition,...
Resveratrol is a potent activator of NAD-dependent deacetyltransferase sirtuin-1 (SIRT1) and affects lipid metabolism ATP generation in somatic cells. In the present study, effects supplementing culture medium with resveratrol on metabolism, generation, cryosensitivity bovine vitro produced embryos were investigated. Bovine early cleaved-stage cultured containing 0 or 0.5 µM for 1 5 days. treatment both day days increased expression levels SIRT1 phosphorylated AMP-activated protein kinase...
Endometrial cells secrete various cytokines and the dysfunction of endometrial may directly lead to infertility. Interferon tau (IFNT) secreted by trophoblast cells, a well-known pregnancy recognition signal in ruminants, acts on uterus prepare for pregnancy. Aging causes cellular organ dysfunction, advanced maternal age is associated with reduced fertility. However, few studies have investigated age-dependent changes uterus. Using next generation sequencing real-time PCR, we examined mRNA...
Blastocyst formation gives rise to the inner cell mass (ICM) and trophectoderm (TE) is followed by differentiation of epiblast (Epi) primitive endoderm (PrE) within ICM. Although these two-round lineage differentiations underpin proper embryogenesis in every mammal, their spatiotemporal dynamics are quite diverse among species. Here, molecular details blastocyst stage cattle were dissected using an optimized vitro culture method. embryos placed on agarose gel filled with nutrient-rich media...
SUMMARY A major difference between in vivo and vitro follicle culture is the stiffness of substrate which follicles grow. In this study, we examined effect polyacrylamide gel (PAG), as a substrate, on development porcine oocytes derived from early antral follicles. Oocyte‐granulosa cell complexes (OGCs) were collected gilts, incubated individually for 14 days 96‐well plate without or with PAG. We then evaluated number granulosa cells present OGCs well abundance ATP, lipid, acetylated lysine...