A5020012457- Reproductive Physiology in Livestock
- Reproductive Biology and Fertility
- Reproductive System and Pregnancy
- Pregnancy and preeclampsia studies
- Birth, Development, and Health
- Inflammasome and immune disorders
- Sperm and Testicular Function
- Tissue Engineering and Regenerative Medicine
- Renal and related cancers
- Angiogenesis and VEGF in Cancer
- Animal health and immunology
- Fatty Acid Research and Health
- IL-33, ST2, and ILC Pathways
- Nitric Oxide and Endothelin Effects
- Sirtuins and Resveratrol in Medicine
- Ovarian function and disorders
- Genetic and phenotypic traits in livestock
- Neonatal Respiratory Health Research
- Pregnancy-related medical research
- Veterinary Equine Medical Research
- Pregnancy and Medication Impact
- Mitochondrial Function and Pathology
- Genetics, Aging, and Longevity in Model Organisms
- Immune Response and Inflammation
- Pluripotent Stem Cells Research
Tokyo University of Agriculture
2016-2025
GTx (United States)
2019-2021
Jichi Medical University
2012-2018
University of Tsukuba
2018
Kitasato University
2018
Yokohama City University
2018
University of Agriculture Faisalabad
2015
Obihiro University of Agriculture and Veterinary Medicine
2005-2014
Kurume University
2014
Shinshu University
2014
Objective— Abdominal aortic aneurysm (AAA) is considered a chronic inflammatory disease; however, the molecular basis underlying sterile response involved in process of AAA remains unclear. We previously showed that inflammasome, which regulates caspase-1–dependent interleukin-1β production, mediates cardiovascular responses. Therefore, we hypothesized inflammasome key mediator initial inflammation formation. Approach and Results— Apoptosis-associated speck-like protein containing caspase...
Abstract Inflammation plays a key role in the pathophysiology of hepatic ischemia–reperfusion (I/R) injury. However, mechanism by which I/R induces inflammatory responses remains unclear. Recent evidence indicates that sterile response triggered is mediated through multiple-protein complex called inflammasome. Therefore, we investigated inflammasome injury and found stimuli upregulated inflammasome-component molecule, nucleotide-binding oligomerization domain–like receptor (NLR) family pyrin...
Objective— Inflammation provoked by the imbalance of fatty acid composition, such as excess saturated acids (SFAs), is implicated in development metabolic diseases. Recent investigations suggest possible role NLRP3 (nucleotide-binding oligomerization domain, leucine-rich repeat and pyrin domain containing 3) inflammasome, which regulates IL-1β (interleukin 1β) release leads to inflammation, this process. Therefore, we investigated underlying mechanism SFAs trigger inflammasome activation....
In cows, interferon-tau (IFNT) regulates maternal recognition around days 15-19 after artificial insemination (AI). The present study hypothesized that if key target genes of IFNT are clearly upregulated in earlier stages pregnancy, these could be use as indices future pregnancy cows. Therefore, we determined the expression peripheral blood mononuclear leukocytes (PBMCs) and polymorphonuclear granulocytes (PMNs) during period (MRP). Twenty multiparous Holstein cows were subjected to AI on...
Abstract Background After ovulation in the cow, corpus luteum (CL) rapidly develops within a few days with angiogenesis and progesterone production. CL formation resembles an inflammatory response due to influx of immune cells. Neutrophils play role host defense inflammation, secrete chemoattractants stimulate angiogenesis. We therefore hypothesized that neutrophils infiltrate developing from just after may CL. Methods Results Polymorphonuclear (PMN) were detected tissue by Pas-staining,...
Mitochondrial numbers increase during oocyte growth. In this study, we collected oocytes and granulosa cell complexes (OGCs) from early antral follicles (EAFs) of aged cows (> 120 months age) examined the effects resveratrol on mitochondrial generation, degradation, quality in grown vitro. We also gene expression cells. Resveratrol (20 µM) enhanced SIRT1 induced autophagy both cells derived cows. Culturing OGCs with increased DNA copy Furthermore, ATP content improved developmental ability...
Despite the increasing commercial use of nanoparticles, little is known about their effects on placental inflammation and pregnancy complications. In this study, nanosilica (NS) particles upregulated inflammasome component nucleotide-binding oligomerization domain-like receptor (NLR) family pyrin domain-containing 3 (NLRP3) induced reactive oxygen species (ROS) generation, resulting in Furthermore, NS-induced complications were markedly improved Nlrp3−/− mice but not apoptosis-associated...
Follicle development is accompanied by proliferation of granulosa cells and increasing oocyte size. To obtain high-quality oocytes in vitro, it important to understand the processes that occur during follicle differences between vivo vitro development. In present study, were collected from early antral follicles (EAFs, 0.5–0.7 mm diameter), small (SAFs, 1–3 large (LAFs, 3–7 grown oocyte-and-granulosa cell complexes (OGCs), which cultured for 14 days after collection EAFs. Gene expression was...
NLRP3 inflammasomes recognize non-microbial danger signals and induce release of proinflammatory cytokine interleukin (IL)-1β, leading to sterile inflammation in cardiovascular disease. Because is involved doxorubicin (Dox)-induced cardiotoxicity, we investigated the role Dox-induced cardiotoxicity. Cardiac dysfunction injury were induced by low-dose Dox (15 mg/kg) administration NLRP3-deficient (NLRP3(-/-)) mice but not wild-type (WT) IL-1β(-/-) mice, indicating that deficiency enhanced...
Macrophages are essential for regulating the physiology of pregnancy; however, excessive inflammatory responses to macrophages, induced by infection and/or endogenous danger signals, may potentially result in complications during pregnancy. Advanced glycation end-products (AGE) and lipopolysaccharides (LPS) known induce inflammation associated with adverse developmental outcomes. The aim present study was examine effect AGE LPS on cytokines J774 murine macrophage cell line potential...
Abstract Purpose Oocyte and embryo quality differs significantly among individuals. Follicular fluid (FF) is a solo environment of oocyte maturation may flux into the oviduct. Supplementation in vitro (IVM) culture (IVC) medium with extracellular vesicles FFs supports embryonic development. We addressed hypothesis that miRNA profiles are crucial background Methods were collected from ovaries individual cows, classified Good or Poor FF based on developmental rate to blastocyst stage enclosed...
Abstract Active angiogenesis and progesterone (P) synthesis occur in parallel during development of the corpus luteum (CL). Basic fibroblast growth factor (bFGF) vascular endothelial (VEGF) are known to stimulate P vitro. The aim present study was investigate impact bFGF or VEGF on CL cow by using a specific antibody against VEGF. antibody, saline as control (n = 4 cows/treatment) were injected directly into immediately after ovulation (Day 1), treatment continued for 3 times/day over 7...
When pregnancy is established, interferon tau (IFNT), a well-known recognition signal in ruminants, secreted by embryonic trophoblast cells and acts within the uterus to prepare for pregnancy. IFNT as an endocrine factor on corpus luteum (CL) induce refractory ability against luteolytic action of PGF2 α. Hypothesising that may influence not only uterine environment but also CL cows via local or peripheral circulation, we investigated qualitative changes pregnant during maternal period (day...
Preeclampsia is a pregnancy-specific syndrome characterized by elevated blood pressure, proteinuria, and intrauterine growth restriction (IUGR). Although sterile inflammation appears to be involved, its pathogenesis remains unclear. Recent evidence indicates that mediated through the nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3 (NLRP3) inflammasomes, composed of NLRP3, apoptosis-associated speck-like protein containing caspase recruitment domain...
Supplemental oxygen inhalation is frequently used to treat severe respiratory failure; however, prolonged exposure hyperoxia causes hyperoxic acute lung injury (HALI), which induces distress syndrome and leads high mortality rates. Recent investigations suggest the possible role of NLRP3 inflammasomes, regulate IL-1β production lead inflammatory responses, in pathophysiology HALI; their not fully understood. In this study, we investigated inflammasomes mice with HALI. Under conditions,...