The primary aim of the present study was to examine effect maternal age (in months) on mitochondrial DNA copy number (Mt number), ATP content and IVF outcome bovine oocytes. We also compared Mt oocytes with fertilisation content. Oocytes were collected from cows aged 20-204 months determined by real-time polymerase chain reaction. in immature mature be 368,118 807,794, respectively; these 1.2 2.0 pM, respectively. Both increased during oocyte maturation. However, after 90 age, decreased...

The aim of the present study was to address effect resveratrol-mediated upregulation sirtuin 1 (SIRT1) during oocyte maturation on mitochondrial function, developmental ability oocytes and mechanisms responsible for blockage polyspermic fertilization. Oocytes collected from slaughterhouse-derived ovaries were cultured in TCM-199 medium supplemented with 10% FCS 0 or 20 µM resveratrol (Res). We examined Res SIRT1 expression vitro-matured (Exp 1); fertilization 2); DNA copy number (Mt number),...

The present study assessed the effect of resveratrol on expression SIRT1 and mitochondrial quality quantity in porcine oocytes. Supplementing maturation medium with 20 µM increased SIRT1, enhanced functions, as observed from ATP content membrane potential. Addition also improved ability oocytes to develop into blastocyst stage following activation. effects number were examined by comparing DNA copy (Mt number) between group collected same donor gilt ovaries. only did not affect Mt However,...

Mitochondrial numbers increase during oocyte growth. In this study, we collected oocytes and granulosa cell complexes (OGCs) from early antral follicles (EAFs) of aged cows (> 120 months age) examined the effects resveratrol on mitochondrial generation, degradation, quality in grown vitro. We also gene expression cells. Resveratrol (20 µM) enhanced SIRT1 induced autophagy both cells derived cows. Culturing OGCs with increased DNA copy Furthermore, ATP content improved developmental ability...

Follicle development is accompanied by proliferation of granulosa cells and increasing oocyte size. To obtain high-quality oocytes in vitro, it important to understand the processes that occur during follicle differences between vivo vitro development. In present study, were collected from early antral follicles (EAFs, 0.5–0.7 mm diameter), small (SAFs, 1–3 large (LAFs, 3–7 grown oocyte-and-granulosa cell complexes (OGCs), which cultured for 14 days after collection EAFs. Gene expression was...

Macrophages are essential for regulating the physiology of pregnancy; however, excessive inflammatory responses to macrophages, induced by infection and/or endogenous danger signals, may potentially result in complications during pregnancy. Advanced glycation end-products (AGE) and lipopolysaccharides (LPS) known induce inflammation associated with adverse developmental outcomes. The aim present study was examine effect AGE LPS on cytokines J774 murine macrophage cell line potential...

Abstract Purpose Oocyte and embryo quality differs significantly among individuals. Follicular fluid (FF) is a solo environment of oocyte maturation may flux into the oviduct. Supplementation in vitro (IVM) culture (IVC) medium with extracellular vesicles FFs supports embryonic development. We addressed hypothesis that miRNA profiles are crucial background Methods were collected from ovaries individual cows, classified Good or Poor FF based on developmental rate to blastocyst stage enclosed...

In general, maternal age affects the quality of oocytes and embryos. The present study aimed to examine features age-associated gene expression profiles bovine embryos as well discover possible countermeasures against events. Comprehensive assays germinal vesicle metaphase II (MII)-stage 8- 16-cell-stage were conducted using next-generation sequencing technology. aged cows showed high genes related oxidative phosphorylation, eIF4 p70S6K signaling, mitochondrial dysfunction in MII-stage...

Low oocyte quality is a possible causal factor of obesity-induced infertility. High palmitic acid (PA) concentration in follicular fluid crucial feature noted obese women. This study examined how high PA reduced mitochondrial oocytes and investigated countermeasure against dysfunction. Cumulus cell–oocyte complexes were obtained from the ovaries gilts, incubated medium containing (0.5 mM) or vehicle (BSA) for 44 h. Culturing at induced dysfunction determined by reactive oxygen species low...

The present study investigated the vitrification-induced deterioration of mitochondrial functions that may reduce developmental ability post-warming bovine embryos. In addition, effect supplementation culture medium with resveratrol on and embryonic development was examined. Two days after in vitro fertilization, embryos 8–12 cells (referred to hereafter as 8-cell embryos) were vitrified warmed, followed by incubation for 5 a containing either vehicle or 0.5 μM resveratrol. Vitrification...

The concentrations of electrolytes (Na, K, Cl, Mg and Ca) glucose in small follicle (SF) follicular fluid (SFF) large (LF) (LFF) from slaughterhouse-derived ovaries were studied. Oocytes matured medium based on synthetic oviductal fluid. effects various Ca Mg) the maturation progression nuclear subsequent development also K SFF was significantly greater than that LFF. concentration (FF) is 2.0–2.3 mM, which present generally used for culture. FF about 3.5–3.9 mM rapidly decreases during...

Progression of meiotic division in oocytes and early embryonic development are affected by quality. In most mammals, oocyte quality declines with increase maternal age. The main aim the present study is to investigate effect age on developmental competence, progression division, associated kinetics maturation promoting factor (MPF) activity bovine oocytes. Oocytes were collected from ovaries young old cows (here after referred as cow oocytes, respectively). When matured fertilized vitro,...

Problem Advanced glycation end products ( AGE s) and high‐mobility group box‐1 HMGB 1) are considered contributing to placental inflammation. We examined the effect of s 1 on cytokines from Sw.71 human trophoblast cell lines interactions between cells THP ‐1‐monocytes. Methods study were cultured with/without or 1. role migration IL ‐6 using co‐cultures conditioned medium ‐1 cells. Results increased interleukin (IL)‐6, ‐8, chemokine C‐C motif ligand 2 CCL 2) secretion The was dependent...

Maternal age affects the quality of oocytes. The present study examined whether follicular fluid (FF) is a casual factor for age-associated decline in oocyte quality. First, we measured concentration advanced glycation end-products (AGE) FF derived from young (21-45 months; Young-FF) and aged (≥120 Aged-FF) cows found significantly higher concentrations AGE Aged-FF than Young-FF. Second, oocytes were collected ovaries or cultured maturation medium containing 10% cows. Regardless origin,...

Abstract Problem Systemic inflammation induced by infection, which is associated with testicular inflammation, predisposes males to subfertility. Recently, the nucleotide‐binding oligomerization domain, leucine‐rich repeat‐, and pyrin domain‐containing 3 (NLRP3) inflammasome was identified as a key mediator of excessive activation NLRP3 shown contribute pathogenesis wide variety diseases. However, mechanisms underlying infectious in testis remain unclear. We investigated effect...

Age-associated deterioration in both the quality and quantity of mitochondria occurs older women. The main aim this study was to examine effect age on mitochondrial DNA copy number (mtDNA number) early developmental stage bovine embryos as well dynamics mtDNA during embryo development. Real-time PCR used determine number. In vitro-produced 48 h after insemination derived from Japanese black cows, ranging 25 209 months were categorized based their cleavage status. There an overall negative...