A5018252207- Organ Transplantation Techniques and Outcomes
- Tissue Engineering and Regenerative Medicine
- Genetic and phenotypic traits in livestock
- Genetic Mapping and Diversity in Plants and Animals
- Organ Donation and Transplantation
- Pluripotent Stem Cells Research
- Virus-based gene therapy research
- Liver physiology and pathology
- Renal and related cancers
- Clinical Nutrition and Gastroenterology
- Liver Disease and Transplantation
- Xenotransplantation and immune response
- Animal Genetics and Reproduction
- Mesenchymal stem cell research
- RNA Interference and Gene Delivery
- Transplantation: Methods and Outcomes
- Renal Transplantation Outcomes and Treatments
- Immunotherapy and Immune Responses
- 3D Printing in Biomedical Research
- Pancreatic function and diabetes
- Cancer Research and Treatments
- Immune Cell Function and Interaction
- Organ and Tissue Transplantation Research
- CRISPR and Genetic Engineering
- Anatomy and Medical Technology
Jikei University School of Medicine
2007-2025
Kanazawa University
2003-2025
Oita University
2023-2025
Keio University
2015-2024
Jichi Medical University
2010-2024
Institute of Livestock and Grassland Science
2015-2024
Obayashi (Japan)
1996-2024
National Agriculture and Food Research Organization
2013-2024
Nippon Veterinary and Life Science University
2020-2024
Advanced Neural Dynamics (United States)
2024
Recently, the field of tissue engineering has progressed rapidly, but poor vascularization remains a major obstacle in bioengineering cell-dense tissues, limiting viable size constructs due to hypoxia, nutrient insufficiency, and waste accumulation. Therefore, new technologies for fabricating functional tissues with well-organized vasculature are required. In present study, neonatal rat cardiomyocytes were harvested as intact sheets from temperature-responsive culture dishes stacked into...
Artificially engineered tissues may have many therapeutic applications but complex are hard to create in vitro. Here, Okano and colleagues report the production of functional cardiac tissue sheets with perfusable blood vessels, which increase thickness survival transplanted tissue. In vitro fabrication vascularized three-dimensional has been a long-standing objective field engineering. Here we technique engineer vessels Using resected connectable artery vein as vascular bed, overlay...
Background— Regenerative therapies, including myocardial tissue engineering, have been pursued as a new possibility to repair the damaged myocardium, and previously transplantation of layered cardiomyocyte sheets has shown be able improve cardiac function after infarction. We examined effects promoting neovascularization by controlling densities cocultured endothelial cells (ECs) within engineered tissues created using our cell sheet-based engineering approach. Methods Results— Neonatal rat...
Regenerative therapies have currently emerged as one of the most promising treatments for repair damaged heart. Recently, numerous researchers reported that isolated cell injection can improve heart function in myocardial infarction models. However, significant loss due to primary hypoxia or wash-out and difficulty control location grafted cells remains problem. As an attempt overcome these limitations, we proposed sheet-based tissue engineering, which involves stacking confluently cultured...
ObjectiveWe investigated the effects of single or repetitive intra-articular injections synovial mesenchymal stem cells (MSCs) on a rat osteoarthritis (OA) model, and elucidated behaviors underlying mechanisms after injection.DesignOne week transection anterior cruciate ligament (ACL) wild type Lewis rats, one million MSCs were injected into knee joint every week. Cartilage degeneration was evaluated with safranin-o staining first injection. To analyze cell kinetics MSC properties,...
Osteoarthritis in the knees, which can be caused by meniscal defect, constitutes an increasingly common medical problem. Repair for massive defect remains a challenge owing to lack of cell kinetics menisci precursors knee joint. The synovium plays pivotal roles during natural course healing and contains mesenchymal stem cells (MSCs) with high chondrogenic potential. Here, we investigated whether intra-articular injected synovium-MSCs enhanced regeneration rat defect. To track cells,...
Meiosis is a critical stage of gametogenesis in which alignment and synapsis chromosomal pairs occur, allowing for the recombination maternal paternal genomes. Here we show that FK506 binding protein (Fkbp6) localizes to meiotic chromosome cores regions homologous synapsis. Targeted inactivation Fkbp6 mice results aspermic males absence normal pachytene spermatocytes. Moreover, identified deletion exon 8 as causative mutation spontaneously male sterile / mutant rats. Loss abnormal pairing...
Abstract Neural stem/progenitor cells (NSPCs) migrate toward a damaged area of the central nervous system (CNS) for purpose limiting and/or repairing damage. Although this migratory property NSPCs could theoretically be exploited cell-based therapeutics CNS diseases, little is known mechanisms responsible responses NSPCs. Here, we found that sphingosine 1-phosphate (Sph-1-P), physiological lysophospholipid mediator, had potent chemoattractant activity NSPCs, in which, Sph-1-P receptors, S1P1...
Ischemia-reperfusion (I/R) injury associated with living donor liver transplantation impairs graft regeneration. Mesenchymal stem cells (MSCs) are potential cell therapeutic targets for disease. In this study, we demonstrate the impact of MSCs against hepatic I/R and hepatectomy.We used a new rat model in which major hepatectomy was performed. Male Lewis rats were separated into two groups: an MSC group given after reperfusion as treatment, Control phosphate-buffered saline placebo. The...
Abstract IFN-λ 1, -λ 2 and 3 have been discovered as the latest members of class II cytokine family shown to possess antiviral activity. Murine B16 melanoma Colon26 cancer cells were transduced with mouse determine whether possesses antitumor Overexpression induced cell surface MHC I expression Fas/CD95 Ag, significant caspase-3/7 activity, increased p21Waf1/Cip1 dephosphorylated Rb (Ser780) in vitro. tumor lines markedly inhibited s.c. metastatic formation vivo compared mock transfections...
To improve the welfare of experimental animals, investigators seek to respect 3R principle (Replacement, Reduction, and Refinement). Even when large animal studies are essential before moving clinical trials, it is important look for ways reduce number animals used. At Center Development Advanced Medical Technology, we consider 'medical' pigs be ideal preclinical model systems. We have been using both wild-type genetically modified pigs. began this approach about 10 years ago with a 'total...
The quality of fat is an important factor in defining the meat. Fat determined by composition fatty acids. Among lipid metabolism-related genes, including acid synthesis several genetic variations have been reported bovine synthase (FASN), stearoyl-CoA desaturase (SCD), sterol regulatory element-binding protein 1 (SREBP1), and GH genes. In present study, we evaluated single epistatic effects 5 (4 SNP insertion/deletion) 4 genes (FASN, SCD, SREBP1, GH) on longissimus thoracis muscle carcass...
Abstract Regulation of plant growth and development by light wavelength has been extensively studied. Less attention paid to effect on formation metabolites. The objective this study was investigate whether volatiles in preharvest postharvest tea ( Camellia sinensis ) leaves can be regulated wavelength. In the present study, contrast natural or dark treatment, blue (470 nm) red (660 significantly increased most endogenous including volatile fatty acid derivatives (VFADs),...
Background aimsTransplantation of synovial mesenchymal stromal cells (MSCs) may induce repair cartilage defects. We transplanted MSCs into defects using a simple method and investigated its usefulness process in pig modelMethodsThe chondrogenic potential the porcine was compared vitro. Cartilage were created both knees seven pigs, divided treated non-treated control knees. Synovial injected defect, knee kept immobilized for 10 min before wound closure. To visualize actual delivery adhesion...
Conventionally, in vitro–fertilized (IVF) bovine embryos are morphologically evaluated at the time of embryo transfer to select those that likely establish a pregnancy. This method is, however, subjective and results unreliable selection. Here we describe novel selection system for IVF blastocysts traces development individual with time-lapse cinematography our developed microwell culture dish analyzes embryonic metabolism. The can noninvasively identify prognostic factors reflect not only...
Anatomic and physiological similarities to the human make swine an excellent large animal model for health disease.Cloning from a modified somatic cell, which can be determined in cells prior making animal, is only method available production of targeted modifications swine.Since some strains are similar size humans, technologies that have been developed readily adapted humans vice versa. Here importance as biomedical model, current produce genetically enhanced swine, models, how completion...