A5051836629- Epigenetics and DNA Methylation
- Genetic Syndromes and Imprinting
- Reproductive Biology and Fertility
- Prenatal Screening and Diagnostics
- Pluripotent Stem Cells Research
- Animal Genetics and Reproduction
- Ovarian function and disorders
- Advanced biosensing and bioanalysis techniques
- Renal and related cancers
- Sperm and Testicular Function
- Chromosomal and Genetic Variations
- MicroRNA in disease regulation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Polymer Nanocomposites and Properties
- Genomics and Chromatin Dynamics
- Alzheimer's disease research and treatments
- Reproductive biology and impacts on aquatic species
- RNA modifications and cancer
- Genomic variations and chromosomal abnormalities
- DNA and Nucleic Acid Chemistry
- RNA Interference and Gene Delivery
- Fish Ecology and Management Studies
- Birth, Development, and Health
- Cancer-related gene regulation
- Tissue Engineering and Regenerative Medicine
Tokyo University of Agriculture
2012-2022
Kyoto University
1985-2020
Noda Institute for Scientific Research
2007
Bio-oriented Technology Research Advancement Institution
2004-2007
Gunma University
2000-2003
Genome-wide dynamic changes in DNA methylation are indispensable for germline development and genomic imprinting mammals. Here, we report single-base resolution methylome transcriptome maps of mouse germ cells, generated using whole-genome shotgun bisulfite sequencing cDNA (mRNA-seq). Oocyte genomes showed a significant positive correlation between mRNA transcript levels the transcribed region. Sperm had nearly complete coverage methylation, except CpG-rich regions, negative gene expression...
Reconstituting gametogenesis in vitro is a key goal for reproductive biology and regenerative medicine. Successful reconstitution of primordial germ cells spermatogenesis has recently had significant effect the field. However, recapitulation oogenesis remains unachieved. Here we demonstrate first reconstitution, to our knowledge, entire process mammalian from cells, using an estrogen-receptor antagonist that promotes normal follicle formation, which turn crucial supporting oocyte growth. The...
In mammals, some genes categorized as imprinted are exclusively expressed either from maternal or paternal allele. This parental‐origin‐specific gene expression is regulated by epigenetic modification of DNA methylation in differentially methylated region (DMR), which independently imposed during oogenesis and spermatogenesis. It known that DMR the female germ line established oocyte growth phase. However, cause progression on DMR, due to aging mice growth‐size was unclear up now. Here, we...
Parthenogenetic embryos, which contained one genome from a neonate-derived non-growing oocyte and the other fully grown oocyte, developed to day 13.5 of gestation in mice, 3 days longer than previously recorded for parthenogenetic development. To investigate hypothesis that disruption primary imprinting during growth leads modified expression imprinted genes this phenotype, we have examined Peg1/Mest, Igf2, Peg3, Snrpn, H19, Igf2r excess p57KIP2. We show paternally expressed genes, Peg3 are...
The ability of maternal chromatin to support full-term development is attained during oocyte growth. aim this study was identify when the growth phase developed capacity term development. Mature metaphase II-arrested oocytes that contained from at different stages were constructed by micromanipulation. fertilized in vitro, blastocyst stage and transferred recipients assay developmental potential. results demonstrate, firstly, origin has no effect on rate maturation, fertilization, or vitro....
The cluster of imprinted genes located in the Dlk1-Dio3 domain spanning 1 Mb plays an essential role controlling pre- and postnatal growth differentiation mice humans. failure parent-of-origin-dependent gene expression this results grave disorders, leading to death some cases. However, little is known about maternally expressed non-coding RNAs (ncRNAs) including many miRNAs snoRNAs domain. In order further understand these ncRNAs, we created Gtl2-mutant harboring a 10 kb deletion exons 1-5....
In mammals, X-chromosome inactivation occurs in all female cells, leaving only a single active X chromosome. This serves to equalise the dosage of X-linked genes male and cells. mouse, paternally derived chromosome (X(P)) is imprinted preferentially inactivated extraembryonic tissues whereas embryonic random. To investigate how X(P) chosen as an we have produced experimental embryos by serial nuclear transplantation from non-growing (ng) oocytes fully grown (fg) oocytes, which chromosomes...
Systematic screening of differentially expressed genes among androgenetic, parthenogenetic, and normal embryos by means fluorescent differential display revealed five imprinted genes. One them, named Rian, was exclusively from the maternal allele closely linked to an gene, Meg3(Gtl2), mapped distal end chromosome 12. The Rian transcript did not have any apparent open reading frame, its localized nucleus.
Mouse parthenogenetic embryos (PEs) are developmentally arrested until embryo day (E) 9.5 because of genomic imprinting. However, we have shown that containing genomes from non-growing (ng) and fully grown (fg) oocytes, i.e. ng wt /fg PE (wt, wild type), developed to E13.5. Moreover, development could be extended term by further regulation Igf2 H19 expression using mice with deletion the transcription unit (H19Δ13) together its differentially (DMR). To gain an insight into parthenotes term,...
The present study shows that the H19 and Igf2r genes, which are imprinted expressed solely from maternal alleles, in an unregulatable manner mouse uniparental, androgenetic, parthenogenetic fetuses at day 9.5 of gestation. In androgenetic fetuses, genes were respectively 12 40% levels biparental fetuses. addition, expression both was excessive (1259 482%, respectively) parthenotes. These expressions not regulated by methylation regulatory regions. Moreover, antisense RNA (Air) also...
Mouse genomes show a large cluster of imprinted genes at the Dlk1-Gtl2 domain in distal region chromosome 12. An intergenic-differentially methylated (IG-DMR) located between Dlk1 and Gtl2 is specifically male germline; IG-DMR regulates parental allele-specific expression genes. Here, we resetting methylation marks during germ-cell differentiation. For analysis, polymorphisms were detected 2.6-kb three mouse strains. Bisulfite analysis showed erasure by E14 re-establishment before birth. The...
In mammals, imprinted genes show parental origin‐dependent expression based on epigenetic modifications called genomic imprinting (GI), which are established independently during spermatogenesis or oogenesis. Due to GI, uniparental fetuses never develop term. To determine whether such of is maintained in mouse fetuses, we analyzed the 20 paternally and 11 maternally expressed androgenetic parthenogenetic fetuses. Four each type were both groups Furthermore, quantitative analysis showed that...
In mammals, genomic imprinting governed by DNA methyltransferase DNMT3A and its cofactor DNMT3L is essential for functional gametes. Oocyte-specific methylation imprints are established during oocyte growth concomitant with DNMT3A/DNMT3L expression, although the mechanisms of oocyte-specific not fully understood. To determine whether presence in oocytes sufficient acquisition imprints, we produced transgenic mice to induce expression prematurely oogenesis analyzed imprints. The results...
We report here on the precise ability of mouse androgenetic embryos produced by in-vitro fertilization enucleated oocytes to develop day 9.5 gestation when cultured with M16 and CZB media. Androgenetic rather than medium developed blastocyst stage in a more significant proportion (56.6% versus 45.0%, P < 0.001). However, after cavitation, rate cell proliferation was significantly decreased (P 0.05). Embryo transfer experiments showed that blastocysts were superior those their 9.5-day-old...
Oligomers of β-amyloid 42 (Aβ42), rather than fibrils, drive the pathogenesis Alzheimer's disease (AD). In particular, toxic oligomeric species called protofibrils (PFs) have attracted significant attention. Herein, we report RNA aptamers with higher affinity toward PFs derived from a Aβ42 dimer fibrils produced WT or toxic, conformationally constrained variant, E22P-Aβ42. We obtained these by using preincubated model E22P-Aβ42, which dimerized via linker located at Val-40, as target in...