A5016703254- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Renal and related cancers
- Epigenetics and DNA Methylation
- Animal Genetics and Reproduction
- Immune Cell Function and Interaction
- Genetic Syndromes and Imprinting
- T-cell and B-cell Immunology
- Breast Lesions and Carcinomas
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Breast Cancer Treatment Studies
- RNA Interference and Gene Delivery
- Reproductive Biology and Fertility
- Chromosomal and Genetic Variations
- 3D Printing in Biomedical Research
- Cancer Genomics and Diagnostics
- Genomic variations and chromosomal abnormalities
- Genetics and Neurodevelopmental Disorders
- Immunotherapy and Immune Responses
- Genomics and Chromatin Dynamics
- Genetic factors in colorectal cancer
- Hernia repair and management
- Esophageal and GI Pathology
- Immune Response and Inflammation
- TGF-β signaling in diseases
Kyoto University
2009-2019
Iwate Medical University
1983-2016
Stem Cell Institute
2015-2016
Juntendo University Shizuoka Hospital
2013-2015
Tachikawa Hospital
2015
Japan Science and Technology Agency
2006-2012
Centre for Research in Engineering Surface Technology
2008
Breast Cancer Research Foundation
2006
The Cancer Institute Hospital
1996-2004
Nha Trang University
1998-2002
The pluripotential cell-specific gene Nanog encodes a homeodomain-bearing transcription factor required for maintaining the undifferentiated state of stem cells. However, molecular mechanisms that regulate expression are largely unknown. To address this important issue, we used luciferase assays to monitor relative activities deletion fragments from 5'-flanking region gene. An adjacent pair highly conserved Octamer- and Sox-binding sites was found be essential activating state-specific...
Abstract Human embryonic stem (ES) cells are predicted to be a valuable source for producing ES‐derived therapeutic spare tissues treat diseases by controlling their growth and differentiation. To understand the regulative mechanisms of differentiation in vivo vitro, ES derived from nonhuman primates could powerful tool. We established four cell lines cynomolgus monkey ( Macaca fascicularis ) blastocysts produced vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). The were...
Following hybridization with embryonic stem (ES) cells, somatic genomes are epigenetically reprogrammed and acquire pluripotency. This results in the transcription of genome-derived tissue-specific genes upon differentiation. During nuclear reprogramming, it is expected that DNA chromatin modifications, believed to function cell-type-specific epigenotype memory, should be significantly modified. Indeed, current evidence indicates acetylation methylation histone H3 H4 amino termini play a...
There are physiological variations in the levels of leucocytes. Among these, circadian rhythm is very important terms magnitude. Since newly identified lymphocyte subsets (i.e. extrathymic T cells) have recently been detected, a comprehensive study was conducted. All leucocytes were found to vary number or proportion with and classified into two groups. One group--granulocytes, macrophages, natural killer (NK) cells, gammadelta CD8+ subset--showed an increase daytime rhythm). The other...
The pluripotency factor Nanog is expressed in peri-implantation embryos and primordial germ cells (PGCs). Nanog-deficient mouse die soon after implantation. To explore the function of cells, RNA was conditionally knocked down vivo by shRNA. shRNA transgenic (NRi-Tg) mice were generated through formation germline chimeras with NRi-Tg embryonic stem cells. In E12.5 Cre-induced ER-Cre/NRi-Tg TNAP-Cre/NRi-Tg double-transgenic embryos, number alkaline phosphatase-positive SSEA1-positive PGCs...
Genomic imprinting directs the allele-specific marking and expression of loci according to their parental origin. Differential DNA methylation at imprinted control regions (ICRs) is established in gametes and, although largely preserved through development, can be experimentally reset by fusing somatic cells with embryonic germ cell (EGC) lines. Here, we show that Ten-Eleven Translocation proteins Tet1 Tet2 participate efficient erasure imprints this model system. The fusion B EGCs initiates...
Mouse liver contains both IL-2Rβ- (or low positive) high T-cell receptor (TCR(hi)) cells and IL-2Rβ+ intermediate TCR (TCR(int)) cells. TCR(int) consist of natural killer 1.1 (NK1)+ NK1- subsets. increase constantly with age whereas TCR(hi) decrease. NK1+ cell proportions in the until middle decrease thereafter. Although other organs are few regardless age, gradually appear lymphoid aging. Skewed usage Vβ7 Vβ8 was observed but predominance less obvious organs. V α14 messenger RNA (mRNA)...
In mammals, X-chromosome inactivation occurs in all female cells, leaving only a single active X chromosome. This serves to equalise the dosage of X-linked genes male and cells. mouse, paternally derived chromosome (X(P)) is imprinted preferentially inactivated extraembryonic tissues whereas embryonic random. To investigate how X(P) chosen as an we have produced experimental embryos by serial nuclear transplantation from non-growing (ng) oocytes fully grown (fg) oocytes, which chromosomes...
Practical clinical applications for current induced pluripotent stem cell (iPSC) technologies are hindered by very low generation efficiencies. Here, we demonstrate that newborn human (h) and mouse (m) extra-embryonic amnion (AM) yolk-sac (YS) cells, in which endogenous KLF4/Klf4, c-MYC/c-Myc RONIN/Ronin expressed, can be reprogrammed to hiPSCs miPSCs with efficiencies AM cells of 0.02% 0.1%, respectively. Both hiPSC indistinguishable from embryonic colony morphology, expression pluripotency...
Abstract Somatic nuclei can be epigenetically reprogrammed by factors present in undifferentiated embryonic stem (ES) cells. The acquisition of pluripotency somatic genomes could render such cells a viable source personalized cell type(s) for therapeutic application, avoiding the need controversial cloning. To investigate this possibility, we first determined origin transcripts teratomas generated from mouse (ES × cell) hybrid clones. Transcription markers genome demonstrated efficient vivo...
X inactivation in female mammals involves transcriptional silencing of an entire chromosome response to a cis-acting noncoding RNA, the inactive-specific transcript (Xist). Xist can also inactivate autosomal sequences, for example, X;autosome translocations; but here, appears be relatively inefficient. This variation has been attributed either attenuated spreading RNA at onset or inefficient maintenance silencing. Evidence date favored latter. Here, we demonstrate T(X;4)37H translocation....
Abstract IL-6 is a cytokine secreted in normal individuals by monocytes, fibroblasts, and endothelial cells. We have found increased levels of the sera from MH134 hepatoma- CSA1M fibrosarcoma-bearing mice. Concerning capacity these tumor cells themselves to produce vitro, they exhibited distinct contrast, i.e., produced high whereas generated marginal level IL-6. It was, however, demonstrated that appreciably enhanced production was observed spleen cell culture supernatants both types...
Embryonic germ-line cells are unipotent that give rise to either sperm or oocytes. However, pluripotent stem can be derived from primordial germ (PGCs) spermatogonia, suggesting retain a capacity for pluripotency. Here, we made genome-wide comparisons of the gene expression profiles freshly isolated PGCs, in vitro-formed PGCs (iPGCs), and other cell lines, including embryonic (ESCs), (EGCs) (GS) cells. Comparing PGC with ESC, 382 genes/transcripts were significantly up-regulated while 188...