A5081228861- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Renal and related cancers
- 3D Printing in Biomedical Research
- Blood Pressure and Hypertension Studies
- Hormonal Regulation and Hypertension
- Sodium Intake and Health
- RNA Interference and Gene Delivery
- Cardiac Valve Diseases and Treatments
- Chromosomal and Genetic Variations
- Lung Cancer Treatments and Mutations
- Race, History, and American Society
- Electrospun Nanofibers in Biomedical Applications
- Fuel Cells and Related Materials
- Historical Gender and Feminism Studies
- Lung Cancer Research Studies
- Myasthenia Gravis and Thymoma
- Brain Metastases and Treatment
- Genomic variations and chromosomal abnormalities
- American Constitutional Law and Politics
- Animal Virus Infections Studies
- Viral Infections and Immunology Research
- SARS-CoV-2 and COVID-19 Research
- Animal Genetics and Reproduction
- Cardiac Structural Anomalies and Repair
Terumo (Japan)
2024-2025
Iwate Medical University
2021
Kyoto University
2009-2019
Arkray (Japan)
2014-2017
Stem Cell Institute
2015-2016
Abstract Excessive salt intake is one of the causes hypertension, and reducing important for managing risk hypertension subsequent cardiovascular events. Esaxerenone, a mineralocorticoid receptor blocker, has potential to exert an antihypertensive effect in hypertensive patients with excessive intake, but evidence still lacking, especially clinical settings. We aimed determine if baseline sodium/potassium ratio estimated 24-h urinary sodium excretion can predict esaxerenone essential...
Abstract The EXCITE-HT study aimed to evaluate the efficacy and safety of esaxerenone versus thiazide diuretics (trichlormethiazide) as second-line treatment for Japanese patients with uncontrolled essential hypertension. This was a 12-week, multicenter, randomized, open-label, parallel-group study. non-inferiority trichlormethiazide confirmed if upper limit two-sided 95% confidence interval (CI) difference in systolic blood pressure (SBP)/diastolic (DBP) change between groups below 3.9/2.1...
Practical clinical applications for current induced pluripotent stem cell (iPSC) technologies are hindered by very low generation efficiencies. Here, we demonstrate that newborn human (h) and mouse (m) extra-embryonic amnion (AM) yolk-sac (YS) cells, in which endogenous KLF4/Klf4, c-MYC/c-Myc RONIN/Ronin expressed, can be reprogrammed to hiPSCs miPSCs with efficiencies AM cells of 0.02% 0.1%, respectively. Both hiPSC indistinguishable from embryonic colony morphology, expression pluripotency...
Messenger RNA (mRNA) has great potential to provide innovative medical solutions in the treatment of heart failure. Although lipid nanoparticles (LNPs) are an established mRNA delivery system, effectively delivering LNPs remains a significant challenge. Here, we evaluated efficacy transcatheter intracoronary (IC) administration compared intravenous (IV) and intramyocardial (IM) normal ischemia-reperfusion (I/R) model rabbit hearts using encapsulating Firefly Luciferase (FLuc) mRNA. In model,...
Human induced pluripotent stem cells (iPSCs) are reprogrammed by transient expression of transcription factors in somatic cells. Approximately 1% can be into iPSCs, while the remaining differentially reprogrammed. Here, we established cancer stem-like (iCSCs) as self-renewing cell clones. Stable iCSC lines were from unstable epithelial (iESC) through re-plating followed embryoid body formation and serial transplantation. iCSCs shared marker genes with except for REX1 LIN28, exhibited EMP1...
Human pluripotent stem cells (hPSCs) are thought to be a promising cell-source solution for regenerative medicine due their indefinite proliferative potential and ability differentiate functional somatic cells. However, issues remain with regard achieving reproducible differentiation of the required functionality realizing human transplantation therapies reducing bacterial or fungal contamination. To meet these needs, we have developed closed-channel culture device corresponding control...
Abstract This prespecified subanalysis of the multicenter, randomized, open-label, parallel-group EXCITE-HT study aimed to examine non-inferiority esaxerenone trichlormethiazide as a second-line antihypertensive agent according basal used (angiotensin receptor blocker [ARB] or calcium channel [CCB]). The primary endpoint, change in morning home systolic/diastolic blood pressure (SBP/DBP) from baseline end treatment was similar between two groups (intergroup difference least squares mean [95%...
Conventional human induced pluripotent stem cells (hiPSCs), reprogrammed from somatic by expression of Oct4, Sox2, Klf4, and c-Myc, are phenotypically different mouse embryonic (ESCs). In mice, culture in N2B27 serum-free 2i media (mitogen-activated protein kinase/extracellular signal-regulated kinase glycogen synthase 3 inhibitors; PD0325901 CHIR99021) plus leukemia inhibitory factor (LIF) (2i+LIF medium) enriches for germline competent ESCs. Here, we demonstrate that flat-shaped hiPSC...
To facilitate understanding the mechanisms of somatic reprogramming to human induced Pluripotent Stem Cells (iPSCs), we have established intermediately Reprogrammed (iRSCs), mesenchymal cells that express exogenous Oct4/Sox2/Klf4/c-Myc (OSKM) and endogenous SOX2/NANOG. iRSCs can be stably maintained at low density. At high density, however, they are enter Mesenchymal-to-Epithelial Transition (MET), resulting in an iPSC state. Morphological changes through MET correlate with silencing OSKM,...
Induced pluripotent stem cells (iPSCs) generated by epigenetic reprogramming of personal somatic have limited therapeutic capacity for patients suffering from genetic disorders. Here we demonstrate restoration a genomic mutation heterozygous Pkd1 (polycystic kidney disease 1) deletion (Pkd1(+/−) to Pkd1(+/R+)) spontaneous mitotic recombination. Notably, recombination between homologous chromosomes occurred at frequency 1∼2 per 10,000 iPSCs. Southern blot hybridization and PCR analyses...
The coronavirus disease 2019 (COVID-19) epidemic remains worldwide. usefulness of the intranasal vaccine and boost immunization against severe acute respiratory syndrome-related (SARS-CoV-2) has recently received much attention. We developed an SARS-CoV-2 by loading receptor binding domain S protein (S-RBD) as antigen into F-deficient Sendai virus vector. After S-RBD-Fd with trimer formation ability was intranasally administered to mice, S-RBD-specific IgM, IgG, IgA, neutralizing antibody...
Introduction: mRNA has great potential to provide new medical innovations in the treatment of heart failure. Lipid nanoparticles (LNPs) are an established and effective delivery system. However, effectively delivering LNPs remains a significant challenge. We evaluated efficacy transcatheter intracoronary (IC) administration compared intravenous (IV) intramyocardial (IM) as methods for delivering. Methods: Normal rabbits were used examine each route administration. Fluorescence (ATTO...
Electro cell fusion has significant potential as a biotechnology tool with applications ranging from antibody production to cellular reprogramming. However due low efficiency of the conventional electro methodology true technique not been reached. In this paper, we report new method which takes two orders magnitude higher than method. The method, based on one-toone pairing, and selection fused cells was developed using microfabricated device. device composed microfluidic channels, micro slit...
Meningeal carcinomatosis in lung cancer is known to have a very poor prognosis. Here we report case which bevacizumab plus erlotinib (BE) was effective against meningeal from afatinib-resistant EGFR mutation-positive cancer. A 61-year-old man started afatinib, 2nd generation molecular targeting drug, as first-line treatment for adenocarcinoma cT1bN0M1a stage IVA harboring exon19 deletion mutation. This shrank the tumor and allowed sustained control of growth. After 19 months start treatment,...
Analysis of gene expression in single cells is required to understand somatic cell reprogramming into human induced pluripotent stem (iPSCs). To facilitate this, we established intermediately reprogrammed (iRSCs), pre-iPSC lines. The iRSC-iPSC conversion system enables the reproducible monitoring events and analysis progressive profiles using single-cell microarray genome editing. Here, showed stage-specific sequential activation during iRSCs iPSCs, OCT4, TDGF1 E-CADHERIN as marker genes....
Nowadays, purulent pericarditis (PP) has become a rare disease with the increased use of antibiotics in clinical practices. The outcome PP is poor due to severe infection and haemodynamic failure by inhibition ventricle repletion, no consensus exists on treatment. We describe case postoperative chronic pericardial effusion changing abscess. Following literature, patient was treated drainage intrapericardial injection urokinase. Inflammation indexes transthoracic echocardiography were...