A5102927120- Pluripotent Stem Cells Research
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- RNA Interference and Gene Delivery
- Nanoparticle-Based Drug Delivery
- Pulsars and Gravitational Waves Research
- Bacteriophages and microbial interactions
- Pleural and Pulmonary Diseases
- Medical Imaging and Pathology Studies
- Neuroscience and Neural Engineering
- Occupational and environmental lung diseases
- Boron Compounds in Chemistry
- Biopolymer Synthesis and Applications
- CRISPR and Genetic Engineering
- Biomedical Ethics and Regulation
- Microbial infections and disease research
- Graphene and Nanomaterials Applications
- Congenital heart defects research
- Geophysics and Gravity Measurements
- Amoebic Infections and Treatments
- Angiogenesis and VEGF in Cancer
- Molecular Communication and Nanonetworks
- Fuel Cells and Related Materials
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Geophysics and Sensor Technology
Osaka University
2011-2025
Osaka University Hospital
2011-2024
Osaka City University
2019
Soka University
2011
City University of Seattle
2008
Despite major therapeutic advances, heart failure, as a non-communicable disease, remains life-threatening disorder, with 26 million patients worldwide, causing more deaths than cancer. Therefore, novel strategies for the treatment of failure continue to be an important clinical need. Based on preclinical studies, allogenic human induced pluripotent stem cell-derived cardiomyocyte (hiPSC-CM) patches have been proposed potential candidate failure. We report implantation allogeneic hiPSC-CM in...
Abstract Background Cell- or tissue-based regenerative therapy is an attractive approach to treat heart failure. A tissue patch that can safely and effectively repair damaged muscle would greatly improve outcomes for patients with In this study, we conducted a preclinical proof-of-concept analysis of the efficacy safety clinical-grade human induced pluripotent stem cell-derived cardiomyocyte (hiPSC-CM) patches. Methods hiPSC line was established using peripheral blood mononuclear cells from...
With the expected increase in patients with heart failure and ischemic 15 cardiomyopathy, development of myocardial regenerative medicine using cell transplantation as a novel treatment method is progressing. This first-in-human clinical trial aimed to confirm safety cardiomyocyte patch derived from allogeneic induced pluripotent stem (iPS) cells based on results several preclinical studies.The inclusion criteria were left ventricular ejection fraction 35% or less; symptoms New York Heart...
Messenger RNA (mRNA) has great potential to provide innovative medical solutions in the treatment of heart failure. Although lipid nanoparticles (LNPs) are an established mRNA delivery system, effectively delivering LNPs remains a significant challenge. Here, we evaluated efficacy transcatheter intracoronary (IC) administration compared intravenous (IV) and intramyocardial (IM) normal ischemia-reperfusion (I/R) model rabbit hearts using encapsulating Firefly Luciferase (FLuc) mRNA. In model,...
A rotating wall vessel (RWV) bioreactor was constructed for growing massive functional cardiac constructs to recover the function of a distressed rat heart. Three-dimensional tissues were engineered by seeding human-induced pluripotent stem cell-derived cardiomyocytes on poly(lactic-co-glycolic acid) fiber sheets (3D-hiPSC-CTs) and cultured in RWV (RWV group) or under static conditions (control group). The transplanted into myocardial infarction nude model, performance evaluated. In group,...
In this study, we created a nanoscale layer of hyaluronic acid (HA) on the inactivated Hemagglutinating Virus Japan envelope (HVJ-E) via layer-by-layer (LbL) assembly technique for CD-44 targeted delivery. HVJ-E was selected as template virus because it has shown tumor-suppressing ability by eliciting inflammatory cytokine production in dendritic cells. Although been required to increase tumor-targeting and reduce nonspecific binding fuses with virtually all cells induces hemagglutination...
Abstract Background Despite major therapeutic advances, heart failure remains a life-threatening disorder, with 26 million patients worldwide, causing more deaths than cancer as non-communicable disease. Therefore, novel strategies for the treatment of continue to be an important clinical need. Based on preclinical studies, allogenic human-induced pluripotent stem cell-derived cardiomyocyte (hiPSC-CM) patches have been proposed potential candidate failure. We report implantation allogeneic...
Abstract Background Boron neutron capture therapy (BNCT) is a cell-selective radiation that uses the alpha particles and lithium nuclei produced by boron reaction. BNCT relatively safe tool for treating multiple or diffuse malignant tumors with little injury to normal tissue. The success failure of depends upon 10 B compound accumulation within tumor cells proximity body surface. To extend therapeutic use from surface visceral will require compounds accumulate strongly in without significant...
We developed a chronic and severe hindlimb ischemia (HLI) mouse model for preclinical research on critical limb (CLI). This partially reflects human CLI pathology in that it does not show spontaneous restoration of blood flow or expression angiogenic genes the ischemic limb. novel may be valuable therapeutic evaluation effects drug candidates.
Abstract Aims Cardiomyocyte-derived induced pluripotent stem cells (iPSCs) may represent a promising therapeutic strategy for severely damaged myocardium. This study aimed to assess the efficacy and safety of clinical grade human iPSC-derived cardiomyocyte (hiPSC-CM) patches conduct pre-clinical proof-of-concept analysis. Methods results A hiPSC line was established from peripheral blood mononuclear collected healthy volunteer homozygous leukocyte antigens differentiated into cardiomyocytes...
A major concern in the clinical application of cell therapy is manufacturing cost products, which mainly depends on quality control. The mycoplasma test, an important biological test therapy, takes several weeks to detect a microorganism and extremely expensive. Furthermore, manual detection from images requires high-level expertise. We hypothesized that identification program using convolutional neural network could reduce time improve sensitivity. To this end, we developed comprising three...
The 5-year survival rate for pancreatic cancer remains low, and the development of new methods its treatment is actively underway. After surgical cancer, recurrence peritoneal dissemination can be prevented by long-term local exposure to appropriate drug concentrations. We propose a novel method using non-woven sheets achieve this goal. Poly(L-lactic acid) containing gemcitabine (GEM) were prepared, GEM sustained release from delivery system was investigated. Approximately 35% dose released...
Introduction: mRNA has great potential to provide new medical innovations in the treatment of heart failure. Lipid nanoparticles (LNPs) are an established and effective delivery system. However, effectively delivering LNPs remains a significant challenge. We evaluated efficacy transcatheter intracoronary (IC) administration compared intravenous (IV) intramyocardial (IM) as methods for delivering. Methods: Normal rabbits were used examine each route administration. Fluorescence (ATTO...
Malignant pleural mesothelioma (MPM) is a refractory tumor because most of the lesions are already disseminated at diagnosis. Previously, main treatment for MPM was combination chemotherapy. However, recently, immune checkpoint inhibitors (ICIs) also used. For better efficacy treatment, we focused on hemagglutinating virus Japan envelope (HVJ-E), which activates antitumor immunity and induces tumor-specific cell death. In this paper, aimed to determine whether HVJ-E as single agent therapy...
Cisplatin (cis-diamminedichloroplatinum (II); CDDP) is a key chemotherapeutic agent but causes renal damage and other off-target effects. Here, we describe the pharmacological biochemical characteristics of novel formulation CDDP complexed with γ-polyglutamic acid (γ-PGA) chitosan (CS), γ-PGA/CDDP-CS, developed by complexing γ-PGA, then adding CS (15 kDa; 10 mol%/γ-PGA). We analyzed tumor cytotoxicity in vitro, as well blood kinetics, acute toxicity, antitumor efficacy vivo BALB/cAJcl mice....
Abstract Introduction How to construct massive cardiac tissue and culture it with functional improvement may be crucial as cardiomyogenesis in failed heart. We previously presented that dynamic a rotating wall vessel (RWV) bioreactor could provide better environment for maintenance of the engineered 3D tissue. However, is unknown about effect cultured RWV on engraftment function distressed rat Hypothesis hypothesized improve its lead recovery infarction model. Methods made by seeding 2.0 ×...
Abstract Cisplatin is the standard anti-tumor drug for mesothelioma, and other malignant tumors, however acute renal failure due to tubule injury often induced as its adverse event. Hence, dose of cisplatin limited by patient's function. We developed controlled release complex that released steadily, relieved event, examined characteristics. 1) Slow from novel Concentration penetrated dialysis membrane was measured ICP atomic emission spectrometer. - Approximately 4% day1 (initial burst very...
The Act on the Safety of Regenerative Medicine enforced in Japan 2014, regulates manufacture cellular processed products. However, with regards to manufacturing facilities at medical institutions, only submission necessary documents is required for a license, and need third-party inspection has been highlighted. Remote activities are becoming more prominent spread Severe Acute Respiratory Syndrome Coronavirus 2 infection; therefore, current assessment compliance structural facility standards...
Introduction: To completely repair the distressed heart with few myocytes it may be crucial how to construct massive engineered three-dimensional cardiac tissues (E3DCTs) and integrate them myocardium after transplant. We hypothesized that E3DCTs maturated by dynamic culture could enhance remodel diseased left ventricle (LV) in rat heart. Methods: made E3DCT seeding 2.0 х 10 6 human induced pluripotent stem cell derived cardiomyocytes on 6mm PLGA fiber sheet. It was cultured a rotating wall...