LOV KELCH PROTEIN2 (LKP2), ZEITLUPE (ZTL)/LOV PROTEIN1 (LKP1) and FLAVIN-BINDING REPEAT F-BOX1 (FKF1) constitute a family of Arabidopsis F-box proteins that regulate the circadian clock. Over-expression LKP2 or ZTL causes arrhythmicity multiple clock outputs under constant light in darkness. Here, we show significance photoperiodic control flowering time Arabidopsis. In plants over-expressing LKP2, CO FT expression was down-regulated long-day conditions. physically interacted with FKF1,...

Abstract Background Autophagy plays an important role in tumour cell growth and survival also promotes resistance to chemotherapy. Hence, autophagy has been targeted for cancer therapy. We previously reported that macrolide antibiotics including azithromycin (AZM) inhibit various types of cells vitro. However, the underlying molecular mechanism inhibition remains unclear. Here, we aimed identify target AZM inhibiting autophagy. Methods identified AZM-binding proteins using AZM-conjugated...

Orf virus (ORFV) is gaining attention as a promising viral vector for cancer therapy because of its unique properties. Recent studies have shown that ORFV could be effective against various cancers, particularly nasopharyngeal carcinoma. This research explores the ability wild-type and recombinant ORFVs, which lack specific virulence factors, to kill NPC cells modulate immune response. Two cell lines, HK1 (from Hong Kong) TW02 Taiwan), were infected with two ORFVs lacking either vascular...

We studied the effects of calcium, cyclic nucleotides, and protein kinase C on albumin transfer, electrical resistance, cytoskeletal actin filaments in cultured human umbilical vein endothelial cells. The monolayer grown collagen-treated filters markedly restricted transfer relative to its across filter alone. Both Ca++ ionophore A23187 ethyleneglycol tetraacetic acid disrupted integrity monolayer, thereby increasing decreasing resistance a concentration-dependent manner. Neither W-7,...

Abstract The levels of endogenous brassinosteroids (BRs) and the expression biosynthesis/metabolism/perception genes involved have been investigated during development germination pea (Pisum sativum) seeds. When seeds were rapidly growing, level biologically active BRs (brassinolide [BL] castasterone [CS]) transcript two BR C-6 oxidases (CYP85A1 CYP85A6) reached a maximum, suggesting significance BL CS in seed development. In early stages germination, CS, but not BL, appeared its increased...

Abstract C-6 oxidation genes play a key role in the regulation of biologically active brassinosteroid (BR) levels plant. They control BR activation, which involves 6-deoxocastasterone (6-DeoxoCS) to castasterone (CS) and some cases further conversion CS brassinolide (BL). is controlled by CYP85A family cytochrome P450s, date, two CYP85As have been isolated tomato (Solanum lycopersicum), Arabidopsis (Arabidopsis thaliana), one rice (Oryza sativa), grape (Vitis vinifera). We now (CYP85A1...

Caffeic acid (CA) and its analogues such as rosmarinic are well known antioxidative agents. Exposure to UVA is generate reactive oxygen species (ROS) singlet (1O2) superoxide anion radical (·O2-) in the skin of animals, which turn induces photodamage photoaging. Because CA quench 1O2, these compounds were topically applied abdominal live hairless mice found suppress ROS generation upon exposure. Furthermore, UVA-induced was also suppressed that orally given CA. In order understand mechanism...

The present study uses structural equation modeling to explore the relationship of child behavior type and its evaluation during dental treatment. population consisted 33 children at their first visit a pediatric dentist Dental Hospital Tsurumi University. Child was evaluated by Frankl Behavior Rating Scale scale developed Kurosu. Factor analysis extracted 3 types: escape, self-defense, facial expression. path diagram relationships between indicated that expression had strongest correlation Scale.

Caffeic acid (CA) and its analogues such as rosmarinic are well known antioxidative agents. Exposure to UVA is generate reactive oxygen species (ROS) singlet (1O2) superoxide anion radical (*O2-) in the skin of animals, which turn induces photodamage photoaging. Because CA quench 1O2, these compounds were topically applied abdominal live hairless mice found suppress ROS generation upon exposure. Furthermore, UVA-induced was also suppressed that orally given CA. In order understand mechanism...

On 1 April 2017, six years have passed since the Fukushima Daiichi Nuclear Power Station (FDNPS) accident, and Japanese government declared that some residents who lived in Tomioka Town, Prefecture could return to their homes. We evaluated environmental contamination radiation exposure dose rates due artificial radionuclides livelihood zone of (living space such as housing sites), including a restricted area located within 10-km radius from FDNPS, immediately after had returned home town. In...

Abstract BRCA1 is a well-studied tumor suppressor involved in the homologous repair of DNA damage, whereas PINK1, mitochondrial serine/threonine kinase, known to be quality control. Genetic mutations PINK1 and Parkin cause autosomal recessive early-onset Parkinson’s disease. We found that breast cancer cells, targeting reagents, which all induce depolarization along with upregulation, induced proteasomal degradation. This degradation was dependent on downregulation upon damage caused...

Orf virus (ORFV) infects sheep and goats is also an important zoonotic pathogen. The viral protein OV20.0 has been shown to suppress innate immunity by targeting the double-stranded RNA (dsRNA)-activated kinase (PKR) multiple mechanisms. These mechanisms include a direct interaction with PKR binding two activators, dsRNA cellular activator (PACT), which ultimately leads inhibition of activation. In present study, we identified novel association between adenosine deaminase acting on 1...

Abstract Temporal variations in ambient dose rates a restricted area designated as “difficult-to-return” for residents of Tomioka Town, Fukushima Prefecture were evaluated car-borne survey during 2018–2019. The median the “Decontaminated area” difficult-to-return zone decreased rapidly from 1.0 μSv/h to 0.32 μSv/h; however, “Non-decontaminated and “Radioactive waste storage fluctuated between 1.1–1.4 0.46–0.61 μSv/h, respectively. detected rate cesium-137 ( 137 Cs) Cs-detected points per all...

Phosphoinositide metabolism participates in the control of cell calcium homeostasis. Because a notable neutral lipid (1,2-diacylglycerol) is generated from phosphoinositide hydrolysis and assumed to be secondary messenger, we determined 1,2-diacylglycerol content its fatty acid profiles thoracic aorta spontaneously hypertensive rats (SHR) compared it with those normotensive Wistar-Kyoto (WKY) rats. After was exposed 10(-5) M norepinephrine as stimulant, SHR significantly higher by 33% than...

Abstract Severe fever with thrombocytopenia syndrome virus (SFTSV) causes the highly fatal disease in humans. To facilitate diagnosis, native form of subunit glycoprotein (Gn), a prime target for potential vaccines and therapies, was produced Nicotiana benthamiana using Bamboo mosaic virus-based vector system. By fusion secretory signal tags, SS Ext , derived from extension protein, (SP) 10 motif, yield recombinant Gn (rGn) remarkably increased to approximately 7 mg/kg infiltrated leaves....

The aim of this study was to examine the effect heat-killed Lactobacillus paracasei NFRI 7415 on kidney and bone in mice fed an ethanol-containing diet with stress. Eight-week-old Cril : CD1 were a control (CD), alcohol (AD) (35.8% total energy from ethanol), or containing 20% Lb. (10 7 CFU/g) (LD) for 4 weeks. Mice AD LD groups also underwent restraint stress two weeks 13 days. placed 50 mL plastic tube, which had small hole drilled around its base allow ventilation, restrained 1 h every...

In recent years, Japan has suffered serious damage due to natural disasters such as earthquakes, heavy rains tropical storms (typhoons) and localized downpours. To assess the chronological changes in attenuation of external exposure doses environmental radiation contamination rainfall associated with typhoons during October December 2019 Fukushima, we measured levels forest areas along Mt Okura hiking trail Tomioka Town, Fukushima Prefecture, near Daiichi Nuclear Power Station. We confirmed...

Summary A case of myelodysplastic syndrome with haemolytic anaemia and a marked elliptocytosis is reported. Sodium dodecyl sulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE) erythrocyte membrane proteins revealed that the patient's band 4.1 was decreased to about 50–70% control contained abnormal molecule migrating in faster mobility than normal 4.1. which confirmed by immunoblotting. The actin/spectrin ratio ghosts diminished 70% ghosts. Flowcytometric analysis showed glycophorin C...