A5009861886- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neuropharmacology Research
- Photoreceptor and optogenetics research
- Cell Adhesion Molecules Research
- Retinal Development and Disorders
- Pluripotent Stem Cells Research
- Axon Guidance and Neuronal Signaling
- Cancer-related gene regulation
- Neuroscience and Neural Engineering
- Cancer Research and Treatments
- Voice and Speech Disorders
- Peptidase Inhibition and Analysis
- Neural dynamics and brain function
- Glycosylation and Glycoproteins Research
- Tracheal and airway disorders
- Head and Neck Cancer Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Colorectal Cancer Surgical Treatments
- Pancreatic and Hepatic Oncology Research
- Ubiquitin and proteasome pathways
- CRISPR and Genetic Engineering
- Melanoma and MAPK Pathways
- Genetic factors in colorectal cancer
- Receptor Mechanisms and Signaling
- Advanced Vision and Imaging
Kanazawa University
2016-2025
Kyushu University
2023-2025
Hokushin General Hospital
2018-2025
Nishinomiya Kyoritsu Neurosurgical Hospital
2025
Ibaraki Prefectural Central Hospital
2006-2024
Kanazawa Medical University
2015-2024
RIKEN Center for Integrative Medical Sciences
2022-2023
Keio University
2022-2023
Osaka Medical and Pharmaceutical University
1994-2017
Interface (United States)
2017
Parkinson disease (PD) is a neurodegenerative disorder characterized by loss of midbrain dopaminergic (DA) neurons. ES cells are currently the most promising donor cell source for cell-replacement therapy in PD. We previously described strong neuralizing activity present on surface stromal cells, named cell–derived inducing (SDIA). In this study, we generated neurospheres composed neural progenitors from monkey which capable producing large numbers DA demonstrated that FGF20, preferentially...
We previously identified a stromal cell-derived inducing activity (SDIA), which induces differentiation of neural cells, including midbrain tyrosine hydroxylase-positive (TH + ) dopaminergic neurons, from mouse embryonic stem cells. report here that SDIA efficient also in primate Induced neurons contain TH at frequency 35% and produce significant amount dopamine. Interestingly, undifferentiated cells occurs much faster vitro (10 days) than it does the embryo (≈5 weeks). In addition, 8%...
In the mammalian central nervous system glutamate is major excitatory neurotransmitter and plays a crucial role in plasticity toxicity of certain neural cells. We found that stimulated activation p38 stress-activated protein kinase (SAPK, also known as c-Jun N-terminal (JNK)), two subgroup members mitogen-activated superfamily matured cerebellar granule The was largely mediated by <i>N</i>-methyl-d-aspartate receptors. Furthermore, we have revealed novel signaling pathway, is,...
To identify the role of survivin, a novel inhibitor apoptosis (IAP) in colorectal tumorigenesis, authors investigated tissue expression survivin human tumors including 43 hyperplastic polyps, 171 adenomas with low dysplasia, 42 high and 60 carcinomas adenoma, examined whether correlated tumor cell apoptosis, proliferation, angiogenesis, which is known to initiate imbalance between proliferation apoptosis.Immunohistochemical staining for paraffin sections by using monoclonal antibodies, p53,...
To understand the range of competence embryonic stem (ES) cell-derived neural precursors, we have examined in vitro differentiation mouse and primate ES cells into dorsal- (neural crest) ventralmost (floor plate) axis. Stromal inducing activity (SDIA; accumulated on PA6 stromal cells) induces cocultured to differentiate rostral CNS tissues containing both ventral dorsal cells. Although early exposure SDIA-treated bone morphogenetic protein (BMP)4 suppresses promotes epidermogenesis, late...
To determine whether primate embryonic stem (ES) cell-derived pigment epithelial cells (ESPEs) have the properties and functions of retinal (RPE) in vitro vivo.Cynomolgus monkey ES were induced to differentiate into by coculturing them with PA6 stromal a differentiating medium. The expanded, single-layer ESPEs examined light electron microscopy. expression standard RPE markers was determined RT-PCR, Western blot, immunocytochemical analyses. transplanted subretinal space 4-week-old Royal...
Context Overexpression of epidermal growth factor (EGF) in the liver induces transformation to hepatocellular carcinoma animal models. Polymorphisms EGF gene modulate levels. Objective To assess relationship among human single-nucleotide polymorphism, expression, and risk carcinoma. Design, Setting, Participants Molecular mechanisms linking 61*G allele polymorphism expression were examined cell lines tissue. A case-control study involving 207 patients with cirrhosis was conducted at...
Two-photon imaging with genetically encoded calcium indicators (GECIs) enables long-term observation of neuronal activity in vivo. However, there are very few studies GECIs primates. Here, we report a method for GECI, GCaMP6f, expressed from adeno-associated virus vectors cortical neurons the adult common marmoset (Callithrix jacchus), small New World primate. We used tetracycline-inducible expression system to robustly amplify GCaMP6f and up- downregulate it more than 100 days. succeeded...
Vocal function following laser surgery for glottic T1a carcinoma was evaluated in 17 patients and compared to vocal radiotherapy 14 patients. The results are summarized as follows. 1) A slight degree of hoarseness found more frequently than radiotherapy. quality rough breathy most cases. 2) In stroboscopic examination, incomplete glottal closure diminution or lack vibration the operated fold were observed surgery. 3) There no marked difference maximum phonation time, mean airflow rate,...
Higher mammals such as primates and carnivores have highly developed unique brain structures the ocular dominance columns in visual cortex, gyrus outer subventricular zone of cerebral cortex. However, our molecular understanding formation, function diseases these is still limited, mainly because genetic manipulations that can be applied to higher are poorly available. Here we validated a rapid efficient technique enables gyrencephalic using utero electroporation. Transgene-expressing ferret...
The c-Jun NH(2)-terminal protein kinase (JNK) plays important roles in a broad range of physiological processes. JNK is controlled by two upstream regulators, mitogen-activated (MKK) 4 and MKK7, which are activated various MAPKKKs. Studies employing knockout mice have demonstrated that the signaling pathway involved diverse phenomena brain, regulating brain development maintenance as well animal metabolism behavior. Furthermore, examination single or combined Jnk1, Jnk2, Jnk3 has revealed...
The CRISPR/Cas9 system has recently been adapted for generating knockout mice to investigate physiological functions and pathological mechanisms. Here, we report a highly efficient procedure brain-specific disruption of genes interest in vivo. We constructed pX330 plasmids expressing humanized Cas9 single-guide RNAs (sgRNAs) against the Satb2 gene, which encodes an AT-rich DNA-binding transcription factor is responsible callosal axon projections developing mouse brain. first confirmed that...
Folds in the cerebral cortex mammals are believed to be key structures for accommodating increased cortical neurons cranial cavity. However, mechanisms underlying folding remain largely unknown, mainly because genetic manipulations gyrencephalic brain have been unavailable. By combining utero electroporation and CRISPR/Cas9 system, we succeeded efficient gene knockout of Cdk5, which is mutated some patients with classical lissencephaly, brains ferrets. We show that Cdk5 ferret markedly...
Although it has been believed that the evolution of cortical folds was a milestone, allowing for an increase in number neurons cerebral cortex, mechanisms underlying formation are largely unknown. Here we show regional differences expression fibroblast growth factor receptors (FGFRs) developing cortex ferrets even before formed. By taking advantage our utero electroporation technique ferrets, found folding impaired ferret when FGF signaling inhibited. We also crucial producing Pax6-positive...
Abstract GABA A receptors (GABA Rs) mediate the majority of fast inhibitory neurotransmission in brain via synergistic association with postsynaptic scaffolding protein gephyrin and its interaction partners. However, unlike their counterparts at glutamatergic synapses, binding partners lack canonical motifs; hence, molecular basis for has remained unclear. In this study, we identify characterize two new posttranslational modifications gephyrin, SUMOylation acetylation. We demonstrate that...
An increase in the diversity of neural progenitor subtypes and folding cerebral cortex are characteristic features which appeared during evolution mammalian brain. Here, we show that expansion a specific subtype is crucial for cortical folding. We found outer radial glial (oRG) cells can be subdivided by HOPX expression gyrencephalic ferrets. Compared with HOPX-negative oRG cells, HOPX-positive had high self-renewal activity were accumulated prospective gyral regions. Using our vivo genetic...
Although distant metastasis from pancreatic ductal adenocarcinoma (PDAC) has a dismal prognosis, some single center studies reported that lung favorable prognosis. The aim of this study is to evaluate the prognostic value site-specific after pancreatectomy for PDAC, with focus on metastasis.Data 117 cases were collected retrospectively 23 institutions in Japan. To compare sites we also data 134 patients liver only metastasis, 67 peritoneal and 121 locoregional recurrence alone.In median time...