A5020447554- Monoclonal and Polyclonal Antibodies Research
- Nanoparticles: synthesis and applications
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- RNA Interference and Gene Delivery
- Angiogenesis and VEGF in Cancer
- Glycosylation and Glycoproteins Research
- Nanoparticle-Based Drug Delivery
- Cell Adhesion Molecules Research
- Advanced Biosensing Techniques and Applications
- HER2/EGFR in Cancer Research
- Graphene and Nanomaterials Applications
- Axon Guidance and Neuronal Signaling
- Advanced biosensing and bioanalysis techniques
- Cancer, Hypoxia, and Metabolism
- T-cell and B-cell Immunology
- Acute Myeloid Leukemia Research
- Allergic Rhinitis and Sensitization
- Virus-based gene therapy research
- Bacteriophages and microbial interactions
- Immune Cell Function and Interaction
- Cytokine Signaling Pathways and Interactions
- Cancer Treatment and Pharmacology
- Toxin Mechanisms and Immunotoxins
- interferon and immune responses
National Institute of Biomedical Innovation, Health and Nutrition
2014-2024
Kobe Gakuin University
2017-2024
Toranomon Hospital
2024
Osaka University
2011-2021
Kanazawa University
2014-2016
National Chung Hsing University
2012
Tokyo University of Science
2008-2011
Tochigi Cancer Center
1994-2011
Northwestern University
2011
University of Modena and Reggio Emilia
2011
Abstract Background Clarifying the physicochemical properties of nanomaterials is crucial for hazard assessment and safe application these substances. With this in mind, we analyzed relationship between particle size vitro effect amorphous nanosilica (nSP). Specifically, evaluated nSP biological effects using human keratinocyte cells (HaCaT). Results Our results indicate that exposure to 70 nm diameter (nSP70) induced an elevated level reactive oxygen species (ROS), leading DNA damage. A...
Structural differences in the binding of tumor necrosis factor to its two receptors may aid development receptor-specific therapeutics.
A safe and potent adjuvant is needed for development of mucosal vaccines against etiological agents, such as influenza virus, that enter the host at surfaces. Cytokines are potential adjuvants because they can enhance primary memory immune responses enough to protect some infectious agents. For this study, we tested 26 interleukin (IL) cytokines vaccine compared their abilities induce antigen (Ag)-specific virus. In mice intranasally immunized with recombinant virus hemagglutinin (rHA) plus...
Abstract Background Due to the rising use of nanomaterials (NMs), there is concern that NMs induce undesirable biological effects because their unique physicochemical properties. Recently, we reported amorphous silica nanoparticles (nSPs), which are one most widely used NMs, can penetrate skin barrier and various effects, including an immune-modulating effect. Thus, it should be clarified whether nSPs a risk factor for aggravation immune diseases. in this study, investigated relationship...
Abstract Surface properties are often hypothesized to be important factors in the development of safer forms nanomaterials (NMs). However, results obtained from studying cellular responses NMs contradictory. Hence, aim this study was investigate relationship between surface silica nanoparticles and their cytotoxicity against a murine macrophage cell line (RAW264.7). The either unmodified (nSP70) or modified with amine (nSP70-N) carboxyl groups (nSP70-C). First, were characterized. RAW264.7...
The CRISPR/Cas9 system has recently been adapted for generating knockout mice to investigate physiological functions and pathological mechanisms. Here, we report a highly efficient procedure brain-specific disruption of genes interest in vivo. We constructed pX330 plasmids expressing humanized Cas9 single-guide RNAs (sgRNAs) against the Satb2 gene, which encodes an AT-rich DNA-binding transcription factor is responsible callosal axon projections developing mouse brain. first confirmed that...
Recent rodent studies have shown that nanoparticles are distributed to breast milk, and more-detailed safety information regarding nanoparticle consumption by lactating mothers is required. Here, we used mice investigate the of use during lactation. When Ag Au were intravenously administered mice, milk without producing apparent damage mammary gland, amount increased with decreasing particle size. Orally also subsequently brains breast-fed pups. Ten-nanometer retained longer in pups' than...
(1 → 3)-β-d-glucan, a characteristic fungal molecule, is known to increase in blood invasive candidiasis, aspergillosis and cryptococcosis. This report shows that the plasma glucan concentration was also elevated four patients infected with Fusarium, Trichosporon beigelii, Saccharomyces cerevisiae Acremonium. In three of patients, elevation preceded positive cultures by 5–17 days, one them it even onset fever 6 days. fourth patient, level decreased clinical improvement. Plasma 3)-β-d-glucan...
Tumor necrosis factor-alpha (TNF) induces inflammatory response predominantly through the TNF receptor-1 (TNFR1). Thus, blocking binding of to TNFR1 is an important strategy for treatment many diseases, such as hepatitis and rheumatoid arthritis. In this study, we identified a TNFR1-selective antagonistic mutant from phage library displaying structural human variants in which each one six amino acid residues at receptor-binding site (amino acids positions 84-89) was replaced with other...
Carcinogenesis is often accompanied by dysfunctional tight junction (TJs), resulting in the loss of cellular polarity. Claudin, a tetra-transmembrane protein, plays pivotal role barrier and fence functions TJs. Claudin-4 deregulated various cancers, including breast, prostate, ovarian, gastric cancer. may be promising target molecule for tumor therapy, but claudin-targeting strategy has never been fully developed. In present study, we prepared claudin-4-targeting fusion C-terminal fragment...
We previously reported that well-dispersed amorphous nanosilicas with particle size 70 nm (nSP70) penetrate skin and produce systemic exposure after topical application. These findings underscore the need to examine biological effects nanosilicas. The present study was designed effects. BALB/c mice were intravenously injected of sizes 70, 100, 300, 1000 then assessed for survival, blood biochemistry, coagulation. As a result, injection nSP70 caused fatal toxicity, liver damage, platelet...
Nanomaterials with particle sizes <100 nm have been already applied in various applications such as cosmetics, medicines, and foods. Therefore, ensuring the safety of nanomaterials is becoming increasingly important. Here we examined localization biological responses intranasally administered amorphous nanosilica particles mice, focusing on coagulation system.We used diameters 30, 70, or 100 (nSP30, nSP70, nSP100 respectively), conventional microscale silica 300 1000 (mSP300 mSP1000,...