A5101484326- Barrier Structure and Function Studies
- Cancer-related molecular mechanisms research
- Gut microbiota and health
- Caveolin-1 and cellular processes
- Neurological Disease Mechanisms and Treatments
- Cell Adhesion Molecules Research
- Glioma Diagnosis and Treatment
- Connexins and lens biology
- Insect and Arachnid Ecology and Behavior
- Cancer Cells and Metastasis
- Pluripotent Stem Cells Research
- Microbial Inactivation Methods
- Genetic diversity and population structure
- Cancer, Hypoxia, and Metabolism
- Ultrasound and Hyperthermia Applications
- Neurogenesis and neuroplasticity mechanisms
- Ubiquitin and proteasome pathways
- Drug Transport and Resistance Mechanisms
- Heat shock proteins research
- Heme Oxygenase-1 and Carbon Monoxide
- PI3K/AKT/mTOR signaling in cancer
- CRISPR and Genetic Engineering
- Lipid metabolism and disorders
- Calpain Protease Function and Regulation
- Advanced Glycation End Products research
Toyama City Hospital
2024
Kanazawa University Hospital
2024
Kanazawa University
2014-2020
Sapporo Medical University
2009-2019
Osaka University of Pharmaceutical Sciences
2017-2018
Oklahoma Medical Research Foundation
2014
University of Tennessee at Knoxville
2014
University of Vermont
2014
Sichuan University
2014
Indiana University – Purdue University Indianapolis
2014
The CRISPR/Cas9 system has recently been adapted for generating knockout mice to investigate physiological functions and pathological mechanisms. Here, we report a highly efficient procedure brain-specific disruption of genes interest in vivo. We constructed pX330 plasmids expressing humanized Cas9 single-guide RNAs (sgRNAs) against the Satb2 gene, which encodes an AT-rich DNA-binding transcription factor is responsible callosal axon projections developing mouse brain. first confirmed that...
An increase in the diversity of neural progenitor subtypes and folding cerebral cortex are characteristic features which appeared during evolution mammalian brain. Here, we show that expansion a specific subtype is crucial for cortical folding. We found outer radial glial (oRG) cells can be subdivided by HOPX expression gyrencephalic ferrets. Compared with HOPX-negative oRG cells, HOPX-positive had high self-renewal activity were accumulated prospective gyral regions. Using our vivo genetic...
The present study investigated the mRNA expression level of ubiquitin c‐terminal hydrolase (UCH)‐L1 and ‐L3 in breast cancer tissue aimed to elucidate its association with tumor characteristics patient prognosis. UCH‐L1 UCH‐L3 levels invasive ( n = 100) were determined by a real‐time polymerase chain reaction (PCR) assay their relationship various clinicopathological tumors as well prognosis studied. was significantly upregulated compared adjacent normal P < 0.005), UHC‐L1 also showed...
Respiratory syncytial virus (RSV) is the major cause of bronchitis, asthma, and severe lower respiratory tract disease in infants young children. The airway epithelium, which has a well-developed barrier regulated by tight junctions, first line defense during infection. In upper human nasal epithelial cells (HNECs), however, primary site RSV infection, mechanisms replication budding RSV, cell responses, including junctional barrier, remain unknown. To investigate detailed HNECs we...
Abstract BACKGROUND Clostridium perfringens enterotoxin (CPE) triggers lysis of epithelial cells through binding to tight‐junction proteins claudin‐3 (Cldn3) and Cldn4, which are over‐expressed in prostate cancer. We investigated the potential Cldn‐targeted therapy using CPE. METHODS expression levels subcellular localization Cldn3 Cldn4 primary human cancer tissues, cell lines (22Rv1, DU145, PC3) normal (PrECs). Cytotoxic effects CPE on these were examined by colorimetric assay. studied...
Abstract Since claudin‐18 (Cldn18) is overexpressed in precursor lesion PanIN and pancreatic duct carcinoma, it serves as a diagnostic marker target of immunotherapy. The stomach isoform Cldn18, Cldn18a2 regulated via PKC/MAPK/AP‐1‐dependent pathway PKC activator 12‐ O ‐tetradecanoylphorbol 13‐acetate (TPA)‐stimulated gastric cancer cells. However, little known about how Cldn18 regulated, not only carcinoma but also normal human epithelial cells (HPDE cells). In the present study, four cell...
Significance Cell adhesion is indispensable for multicellular organisms to form cohesive sheets and serve as tissues organs. In addition the adhesive activity, it exhibits signaling ability organize a variety of cellular processes. However, mechanism by which cell influences events, especially on regulation gene expression, remains obscure. We determine that originated from tight-junction protein claudin-6, targets members nuclear receptor superfamily, such retinoic acid γ estrogen α,...
Angiogenesis is crucial for many pathological processes and becomes a therapeutic strategy against diseases ranging from inflammation to cancer. The regulatory mechanism of angiogenesis remains unclear. Although tetraspanin CD82 widely expressed in various endothelial cells (ECs), its vascular function unknown.Angiogenesis was examined Cd82-null mice with vivo ex morphogenesis assays. Cellular functions, molecular interactions, signaling were analyzed ECs. Angiogenic responses stimuli became...
Aim This study was undertaken to evaluate the expression of claudins 7 and 18 in pancreatic ductal adenocarcinoma. Methods results Material tested included 111 operated samples 47 additional biopsy consisting 26 cases pancreatitis, 3 intraepithelial neoplasia adenocarcinomas. Samples were stained with antibodies analysed for membranous cytoplasmic expression. Membrane bound claudin detected 62 105 (59%) 78 (70%) cases, respectively. associated large or intermediate neoplastic ducts (p=0.01,...
The tetraspanin protein CD53 recruits the kinase PKCβ to plasma membrane activate signaling in B cells.
Tricellulin is an important component of tricellular tight junctions (TJs) and involved in the formation contacts. However, little known about its regulation during assembly disassembly TJs. By using well-differentiated pancreatic cancer cell line HPAC, which highly expresses tricellulin at contacts, we have investigated changes localization, expression phosphorylation TJ functions as a barrier fence destruction TJs induced by extracellular calcium concentration. During both Ca(2+) depletion...