We previously identified a stromal cell-derived inducing activity (SDIA), which induces differentiation of neural cells, including midbrain tyrosine hydroxylase-positive (TH + ) dopaminergic neurons, from mouse embryonic stem cells. report here that SDIA efficient also in primate Induced neurons contain TH at frequency 35% and produce significant amount dopamine. Interestingly, undifferentiated cells occurs much faster vitro (10 days) than it does the embryo (≈5 weeks). In addition, 8%...

To determine whether primate embryonic stem (ES) cell-derived pigment epithelial cells (ESPEs) have the properties and functions of retinal (RPE) in vitro vivo.Cynomolgus monkey ES were induced to differentiate into by coculturing them with PA6 stromal a differentiating medium. The expanded, single-layer ESPEs examined light electron microscopy. expression standard RPE markers was determined RT-PCR, Western blot, immunocytochemical analyses. transplanted subretinal space 4-week-old Royal...

Potential retinal adverse events after COVID-19 vaccinations reported previously include paracentral acute middle maculopathy (PAMM), macular neuroretinopathy (AMN), and central serous chorioretinopathy. We report four cases of branch artery occlusion (BRAO), one case PAMM, AMN that occurred administration the Pfizer-BioNTech vaccine.We retrospectively reviewed medical records six patients who presented to Yame General Hospital or Oita University from July through October 2021.Four (2 males)...

In nonphagocytic cells, Rac1 is a component of NADPH oxidase that produces reactive oxygen species [Ushio-Fukai M (2006) Sci STKE 2006:re8]. expressed abundantly in mammalian retinal photoreceptors, where it activated response to light stimuli [Balasubramanian N, Slepak VZ (2003) Curr Biol 13:1306-1310]. We used Cre-LoxP conditional gene targeting knock down expression mouse rod photoreceptors and found protection against light-induced photoreceptor death compared with WT litter-mates. also...

To produce lens cells from primate embryonic stem (ES) in a reproducible, controlled manner.Cynomologus monkey ES were induced to differentiate by stromal cell-derived inducing activity (SDIA). The lentoids produced this treatment processed for immunohistochemical and immunoblotting analysis. effect of varying the concentration fibroblast growth factor (FGF)-2 density colonies plated during differentiation process also examined.After 2- 3-week induction period, subpopulation colonies....

Abstract Ischemic proliferative retinopathy (IPR), such as diabetic (PDR), retinal vein occlusion and of prematurity is a major cause vision loss. Our previous studies demonstrated that periostin (PN) tenascin-C (TNC) are involved in the pathogenesis IPR. However, interactive role PN TNC angiogenesis associated with IPR remain unknown. We found significant correlation between concentrations PDR vitreous humor. mRNA protein expression were pre-retinal fibrovascular membranes excised from...

The purpose of this study was to investigate the effects various genes related photoreceptor development on rodent and primate iris cells potential as donor for retinal transplantation.Adult rat monkey tissue were cultured in serum-free medium containing basic fibroblast growth factor. Gene deliveries Crx, Nrl, NeuroD some combinations (Crx-Nrl, Crx-NeuroD) performed with recombinant retrovirus. Immunocytochemistry, Western blot analysis, RT-PCR, intracellular recording used examine...

To describe the characteristics and surgical outcomes of pediatric rhegmatogenous retinal detachment.A retrospective study patients (15 years old or younger) who had undergone primary surgery for detachment was conducted. Patients were divided into five groups according to predisposing factors: trauma (group 1), myopia 2), atopic dermatitis 3), congenital developmental anomalies 4), others 5).A total 48 eyes 44 included in this study. There 18 (37.5%) group 1, twelve (25.0%) 2, six (12.5%)...

Intravitreal injection of anti-vascular endothelial growth factor (anti-VEGF) agents reduces microaneurysms in patients with diabetic macular edema (DME). However, residual anti-VEGF-resistant telangiectatic capillaries (TelCaps) have been reported. In this study, we investigated changes the size TelCaps after intravitreal anti-VEGF DME.Indocyanine green angiography (IA) and optical coherence tomography were performed before 3 months (pro re nata regimen three monthly loading doses) 12 eyes...

In Brief Purpose: To evaluate the efficacy of vitrectomy in treatment macular retinoschisis without a detectable optic disk pit. Methods: This retrospective interventional case series included eight patients with acquired, unilateral or foveal detachment. Patients an pit, vitreomacular traction, high myopia were excluded. Six underwent internal limiting membrane peeling and fluid-air exchange. The surgical outcome was evaluated terms improvement anatomy best-corrected visual acuity. Results:...

The purpose of this study was to evaluate the efficacy pars plana vitrectomy for treatment optic disc pit-associated maculopathy with or without preoperative posterior vitreous detachment.We reviewed clinical records four consecutive patients who underwent in one eye maculopathy, an emphasis on condition hyaloid membrane.Two eyes were confirmed have attached membrane, which subsequently removed during surgery. Following vitrectomy, these two experienced improvement visual acuity complete...

Intravitreal injections of anti-vascular endothelial growth factor (VEGF) agents such as brolucizumab and aflibercept are used widely to treat neovascular age-related macular degeneration (nAMD); however; they may theoretically affect the ocular blood flow. We investigated short-term changes in flow between eyes with nAMD treated intravitreal (IVBr) (IVA).This study included 21 Japanese patients either IVBr or IVA at Kurume University Hospital from April 2021 through June 2022. The rates...

The purpose of this study was to investigate the bacterial composition in conjunctiva and determine relationship between fluoroquinolone resistance mutations quinolone resistance-determining region (QRDR) Corynebacterium macginleyi (C. macginleyi).

The purpose of our study was to determine the 4-year visual and anatomic outcomes intravitreal aflibercept treatment for neovascular age-related macular degeneration (AMD) using a treat-and-extend (TAE) regimen.We retrospectively reviewed medical records 39 patients with AMD who were treated continuously injections TAE regimen at least 4 years. outcome measures best-corrected acuity (BCVA) central thickness (CMT) on spectral-domain optical coherence tomography. BCVAs measured as decimal...

Patients with polypoidal choroidal vasculopathy (PCV) may develop large submacular hemorrhages (SMHs), which result in severe visual loss. This study was performed to determine the outcomes and prognostic factors of SMHs secondary PCV.We retrospectively reviewed medical records patients diagnosed PCV who developed a SMH. The best-corrected acuity (BCVA) data were collected at SMH development, 1 month, year after final visit. Patients' information also included age, gender, systemic...

For cell replacement therapies of retinal diseases, the most-needed cells are pigment epithelial and photoreceptor cells; lens needed for cataract. Stromal cell-derived inducing activity induces differentiation mouse embryonic stem (ES) into neural cells, including midbrain tyrosine hydroxylase-positive dopaminergic neurons, ocular method also works with primate ES cells. We describe methods to induce from monkey using stromal show characteristics those in vitro vivo.