A5007439601- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Monoclonal and Polyclonal Antibodies Research
- Cancer Research and Treatments
- Antibiotic Resistance in Bacteria
- Protein purification and stability
- Salmonella and Campylobacter epidemiology
- Estrogen and related hormone effects
- Enzyme Production and Characterization
- HER2/EGFR in Cancer Research
- Biochemical and Structural Characterization
- Viral gastroenteritis research and epidemiology
- Advanced Cellulose Research Studies
- Vibrio bacteria research studies
- Genomics and Phylogenetic Studies
- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Biofuel production and bioconversion
- Receptor Mechanisms and Signaling
- Microbial Inactivation Methods
- Virus-based gene therapy research
- Peptidase Inhibition and Analysis
- Cell Adhesion Molecules Research
Vietnam National University Ho Chi Minh City
2015-2025
Ho Chi Minh City University of Science
2016-2025
Chemical Genomics Centre
2009-2016
Max Planck Institute of Molecular Physiology
2009-2016
Max Planck Society
2009-2014
University of Bayreuth
2005-2013
Oak Ridge National Laboratory
1960
United States Department of Energy
1960
In general, fusion of recombinant genes to strong inducible promoters allowing intracellular expression in Bacillus subtilis is a two-step process. The ligation products are transformed into Escherichia coli, followed by identification the correct plasmid, and this plasmid subsequently B. subtilis. This raises problem that basal level gene could be harmful for E. coli cells. Based on Pgrac promoter, we optimized UP element, -35, 15, -10 +1 region enhance promoter activity after induction....
This study aimed to produce bacterial cellulose from paper waste sludge (PWS) as a method of utilizing the source remaining pulp in material. Initially, PWS was hydrolyzed by sulfuric acid create an enriched-reducing sugar hydrolysate. One-factor experiments were conducted with fixed amount (5 g) investigate influence hydrolysis conditions, including water, addition, temperature, and retention time, on production yield reducing sugars. Based these results, Box-Behnken model designed optimize...
ABSTRACT Colistin resistance threatens global health as it compromises the effectiveness of a last-resort antibiotic. We present complete genome sequence Enterobacter cloacae ST462, which carries mcr-9.1 gene, isolated from pediatric diarrhea case in southern Vietnam. The 5,049,362 bp contains 24 genes distributed across 107 contigs.
Salmonella spp. is a prominent causative agent of pediatric diarrhea, with recent research emphasizing the rising concern regarding antimicrobial resistance (AMR), particularly involving colistin, last-resort antibiotic. This study involved collection 500 stool samples from children diarrhea two tertiary hospitals in Ho Chi Minh City. Conventional methods were used to isolate spp., and antibiotic profiling against seven antibiotics was conducted using disk diffusion method. Colistin...
Bacillus subtilis codes for two putative sortases, YhcS and YwpE, surface proteins, YhcR YfkN, harboring sorting motifs supposed to be recognized by the sortase(s). However, there is no experimental evidence show a direct link between these sortases sequences. To study role of on displaying YfkN cell wall, expression yhcS ywpE was analyzed transcriptional fusions Northern blot. It turned out that gene expressed at higher level during late stationary phase from both experiments, while not...
Nuclear receptor binding to coactivator proteins is an obligate first step in the regulation of gene transcription. receptors preferentially bind LXXLL peptide motif which highly conserved throughout 300 or so natural proteins. This knowledge has shaped current understanding this fundamental protein-protein interaction, and continues inspire search for new drug therapies. However, sequence specificity beyond molecular functioning flanking residues still requires urgent addressing. Here,...
Discoidin domain-containing receptors (DDRs) exhibit a unique mechanism of action among the receptor tyrosine kinases (RTKs) because their catalytic activity is induced by extracellular collagen binding. Moreover, they are essential components in assimilation signals. Recently, DDRs were reported to be significantly linked tumor progression breast cancer facilitating processes invasion, migration, and metastasis. Here, we report successful development fluorescence-based, direct binding assay...
Besides Escherichia coli, Bacillus subtilis is an important bacterial species for the production of recombinant proteins. Recombinant genes are inserted into shuttle expression vectors which replicate in both E. coli and B. subtilis. The ligation products first transformed cells, analyzed correct insertions, plasmids then A major problem using cells can be strong basal level protein may interfere with stability cells. To minimize this problem, we developed being repressed inducer-free In...
Inducer-free integrative vectors are often used to create B. subtilis strains for industrial purposes, but employing strong promoters produce high levels of recombinant proteins in results leaky expression that can hamper cloning Escherichia coli. To overcome the problem, we IPTG-inducible Pgrac harboring lac operators construct inducer-free able integrate into genome at either lacA or amyE locus, both and examined their ability repress β-galactosidase (bgaB) gene E. coli overexpress BgaB...
In addition to the catalytically active kinase domain, most kinases feature regulatory domains that govern their activity. Modulating and interfering with these interdomain interactions presents a major opportunity for understanding biological systems developing novel therapeutics. Therefore, small molecule inhibitors target through an allosteric mode of action have high intrinsic selectivity, as are often unique single or family. Here we report development iFLiK (interface-Fluorescent...
Miniprotein phage display screening yields structured peptides with high affinity for the Estrogen Receptor (ER). Hits from apamin libraries feature a LXXLL motif specifically placed on predefined miniprotein helical segment. The scaffold also allows optimization of flanking amino acids to ensure an optimal ER binding affinity.