A5002264469- Antibiotic Resistance in Bacteria
- Berberine and alkaloids research
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- RNA Interference and Gene Delivery
- Alkaloids: synthesis and pharmacology
- Antimicrobial Peptides and Activities
- Enzyme Catalysis and Immobilization
- Drug Transport and Resistance Mechanisms
- Lipid Membrane Structure and Behavior
- Computational Drug Discovery Methods
- Vibrio bacteria research studies
- Metabolomics and Mass Spectrometry Studies
- Bacterial Genetics and Biotechnology
- Biochemical and Structural Characterization
- Enzyme Production and Characterization
- Bacteriophages and microbial interactions
- Enzyme Structure and Function
- Bacterial biofilms and quorum sensing
- Phenothiazines and Benzothiazines Synthesis and Activities
- Microbial Natural Products and Biosynthesis
- Microbial Community Ecology and Physiology
- Advancements in Transdermal Drug Delivery
- Biochemical Analysis and Sensing Techniques
- Algal biology and biofuel production
Marmara University
2015-2024
Acıbadem Kadıköy Hospital
2010
Postgenomics drug development is undergoing major transformation in the age of multi-omics studies and repositioning. Rather than applications solely personalized medicine, omics science thus additionally offers a better understanding broader range targets Berberine an isoquinoline alkaloid found many medicinal plants. We report here whole genome microarray study tandem with proteomics techniques for mining plethora that are putatively involved antimicrobial activity berberine against...
In the last 20 years, an increasing number of studies have been reported on membrane active peptides, which exert their biological activity by interacting with cell either to disrupt it and lead lysis or translocate through deliver cargos into reach target. These peptides are attractive alternatives currently used pharmaceuticals. Antimicrobial (AMPs) designed for drug gene delivery in pipeline suggest that these will soon constitute a significant percentage market. Here, we focus two most...
The virulence factor Type IV pili (T4P) are surface appendages used by the opportunistic pathogen Pseudomonas aeruginosa for twitching motility and adhesion in environment during infection. Additionally, use of these P. biofilm formation increases its drug resistance. Therefore, attenuation activity T4P would be desirable to control infections. Here, a computational approach has been pursued screen natural products that can this purpose. PilB, elongation ATPase machinery aeruginosa, selected...
Oranges are the most processed fruit in world–it is therefore apparent that industrial production of orange juice generates large quantities peel as a by-product. Unfortunately, management waste leads to economic and environmental problems. Meanwhile, use sustainable raw materials for bulk chemicals, such amino acids, becoming increasingly attractive. To address both issues, this study focused on material media preparation acids by engineered Corynebacterium glutamicum . C. grew pure...
Diacyl glycerophospholipids (GPs) belong to the most abundant lipid species in living organisms and consist of a glycerol backbone with fatty acyl groups sn-1 sn-2 polar head group sn-3 position. Regioisomeric mixed diacyl GPs have same composition but differ their allocation or unit. In-depth analysis regioisomeric GP composed moieties that are similar length degree saturation typically requires either chemical derivatization sophisticated analytical instrumentation, since these types...
Declining efficiency of antibiotic-inhibitor combinatorial therapies in treating beta-lactamase mediated resistance necessitates novel inhibitor development. Allosteric inhibition offers an alternative to conventional drugs that target the conserved active site. Here, we show evolutionarily PWP triad located at N-terminus H10 helix directly interacts with allosteric site TEM-1 and regulates its activity. While point mutations preserve overall secondary structures around site, they result a...
Berberine is a plant-derived alkaloid possessing antimicrobial activity; unfortunately, its efflux through multidrug resistance pumps reduces efficacy. Cellular life span of Escherichia coli generally shorter with prolonged berberine exposure; nevertheless, about 30% the cells still remain robust following this treatment. To elucidate mechanism action and to identify proteins that could be involved in development resistance, protein profiles E. treated for 4.5 8 hours were compared control...
A gram-negative, moderately halophilic bacterium was isolated from Çamaltı Saltern area, located in the Aegean Region of Turkey. Analysis its 16S rRNA gene sequence and physiological characteristics showed that this strain belonged to genus Halomonas ; hence, it designated as sp. AAD12. The isolate tolerated up 800 mg⋅L –1 phenol; however, at elevated concentrations, phenol severely retarded cell growth. increase lag phase with increasing concentrations indicated microorganism undergoing...
Cell-penetrating peptides (CPPs) are commonly defined by their shared ability to be internalized into eukaryotic cells, without inducing permanent membrane damage, and improve cargo delivery. Many CPPs also possess antimicrobial action strong enough selectively lyse microbes in infected mammalian cultures. pVEC, a CPP derived from cadherin, is able translocate it antimicrobial. Structure-activity relationship sequence alignment studies have suggested that the hydrophobic N-terminus (LLIIL)...
Discovery of cargo carrying cell-penetrating peptides has opened a new gate in the development peptide-based drugs that can effectively target intracellular enzymes. Success application and drug design depends on understanding their translocation mechanisms. In this study, our aim was to examine bacterial mechanism pVEC peptide (LLIILRRRIRKQAHAHSK) using steered molecular dynamics (SMD) simulations. The significance specific residues or regions for studied by performing SMD simulations...
Co-administration of beta-lactam antibiotics and beta-lactamase inhibitors has been a favored treatment strategy against beta-lactamase-mediated bacterial antibiotic resistance, but the emergence beta-lactamases resistant to current necessitates discovery novel non-beta-lactam inhibitors. Peptides derived from Ala46–Tyr51 region inhibitor protein are considered as potent beta-lactamase; unfortunately, peptide delivery into cell limits their potential. The properties cell-penetrating peptides...
Discovery of new antibacterials with novel mechanisms is important to counteract the ingenious resistance bacteria. In this connection, omics-guided drug discovery offers a rigorous method in quest antibacterials. (-)-Roemerine plant alkaloid that has been reported possess putative antibacterial activity against Escherichia coli, Bacillus subtilis, and Salmonella typhimurium. The aim present study was characterize (-)-roemerine coli TB1 using proteomics tools. With treatment, we found...
Beta-lactamase-mediated bacterial drug resistance exacerbates the prognosis of infectious diseases, which are sometimes treated with co-administration beta-lactam type antibiotics and beta-lactamase inhibitors. Antimicrobial peptides promising broad-spectrum alternatives to conventional in this era evolving resistance. Peptides based on Ala46–Tyr51 beta-hairpin loop inhibitory protein (BLIP) have been previously shown inhibit beta-lactamase. Here, our goal was modify peptide for improved...
Alkaliphilic organisms are among an industrially important class of extremophile microorganisms with the ability to thrive at pH 10–11.5. Microorganisms that exhibit alkaliphilic characteristics sources alkali-tolerant enzymes such as proteases, starch degrading enzymes, cellulases, and metabolites antibiotics, enzyme inhibitors, siderophores, organic acids, cholic acid derivatives, which have found various applications in industry for human environmental health. Yet, multi-omics mechanisms...