A5064337727- Advanced Fluorescence Microscopy Techniques
- Cardiovascular Health and Risk Factors
- Microtubule and mitosis dynamics
- Cell Image Analysis Techniques
- Advanced Electron Microscopy Techniques and Applications
- Diffusion and Search Dynamics
- bioluminescence and chemiluminescence research
- Genomics and Chromatin Dynamics
- DNA Repair Mechanisms
- Bacteriophages and microbial interactions
- Advanced Biosensing Techniques and Applications
- Single-cell and spatial transcriptomics
- Microbial Community Ecology and Physiology
- Advanced biosensing and bioanalysis techniques
- Digital Holography and Microscopy
- Vibrio bacteria research studies
- Photoreceptor and optogenetics research
- Near-Field Optical Microscopy
- Bacterial biofilms and quorum sensing
- Supramolecular Self-Assembly in Materials
- Animal Behavior and Welfare Studies
- Bacterial Genetics and Biotechnology
- Molecular Communication and Nanonetworks
- CRISPR and Genetic Engineering
- Photochromic and Fluorescence Chemistry
Loewe Center for Synthetic Microbiology
2016-2024
Max Planck Institute for Terrestrial Microbiology
2016-2024
University of Bonn
2022-2024
Carnegie Mellon University
2021-2024
Philipps University of Marburg
2022
Abstract Bacteria use type III secretion injectisomes to inject effector proteins into eukaryotic target cells. Recruitment of effectors the machinery and resulting export hierarchy involve sorting platform. These conserved form pod structures at cytosolic interface injectisome but are also mobile in cytosol. Photoactivated localization microscopy Yersinia enterocolitica revealed a direct interaction platform SctQ SctL with cytosol live bacteria. larger protein complexes involving ATPase...
Abstract Photoconversion of fluorescent proteins by blue and complementary near‐infrared light, termed primed conversion (PC), is a mechanism recently discovered for Dendra2. We demonstrate that controlling the conformation arginine at residue 66 threonine 69 from Anthozoan families (Dendra2, mMaple, Eos, mKikGR, pcDronpa protein families) represents general route to facilitate PC. Mutations alanine 159 or serine 173, which are known influence chromophore flexibility allow reversible...
In recent years, fluorescence microscopy techniques for the localization and tracking of single molecules in living cells have become well-established are indispensable tools investigation cellular biology vivo biochemistry many bacterial eukaryotic organisms. Nevertheless, these still not established imaging archaea. Their establishment as a standard tool study archaea will be decisive milestone exploration this branch life its unique biology. Here, we developed reliable protocol archaeon...
Abstract In quorum sensing, bacteria secrete or release small molecules into the environment that, once they reach a certain threshold, trigger behavioural change in population. As concentration of these so-called autoinducers is supposed to reflect population density, were originally assumed be continuously produced by all cells However, here we show that α-proteobacterium Sinorhizobium meliloti expression autoinducer synthase gene realized asynchronous stochastic pulses result from...
Single-molecule localization microscopy (SMLM) is an advanced method that uses the blinking of fluorescent molecules to determine position these with a resolution below diffraction limit (∼5-40 nm). While SMLM imaging itself becoming more popular, computational analysis surrounding technique still specialized area and often remains "black box" for experimental researchers. Here, we provide introduction required imaging, post-processing typical data analysis. Importantly, user-friendly,...
The key to ensuring proper chromosome segregation during mitosis is the kinetochore (KT), a tightly regulated multiprotein complex that links centromeric chromatin spindle microtubules and as such leads process. Understanding its architecture, function, regulation therefore essential. However, due complexity dynamics, only individual subcomplexes could be studied in structural detail so far. In this study, we construct nanometer-precise situ map of human-like regional KT Schizosaccharomyces...
Super-resolution fluorescence microscopy plays a major role in revealing the organization and dynamics of living cells. Nevertheless, single-molecule localization imaging multiple targets is still limited by availability suitable fluorophore combinations. Here, we introduce novel strategy which combines primed photoconversion (PC) UV-photoactivation for different molecular species tagged fluorescent protein In this approach, proteins can be specifically photoactivated/-converted light...
Abstract Photokonversion von fluoreszierenden Proteinen mithilfe blauem und komplementärem nah‐infraroten Licht, genannt “Primed Conversion” (PC), ist ein kürzlich für Dendra2 entdeckter Mechanismus. Die Kontrolle über die Konformation Arginin an Position 66 Threonin 69 fluoreszierender Proteine aus Anthozoa (Proteinfamilien Dendra2, mMaple, Eos, mKikGR, pcDronpa) ermöglicht in allgemeiner Weise PC. Mutationen Alanin 159 oder Serin 173, dafür bekannt sind, Chromophorflexibilität zu...
Abstract In recent years, fluorescence microscopy techniques for the localization and tracking of single molecules in living cells have become well-established indispensable tools investigation cellular biology vivo biochemistry many bacterial eukaryotic organisms. Nevertheless, these are still not established imaging archaea. Their establishment as a standard tool study archaea will be decisive milestone exploration this branch life its unique biology. Here we developed reliable protocol...
In single-particle tracking (spt), individual particles are localized and tracked over time to probe their diffusion molecular interactions. Temporal crossing of trajectories, blinking particles, false-positive localizations present computational challenges that have remained difficult overcome. Here, we introduce a robust, parameter-free alternative spt: TARDIS. TARDIS, an all-to-all distance analysis between is performed with increasing temporal shifts. These pairwise distances fall in two...
Abstract The key to ensuring proper chromosome segregation during mitosis is the kinetochore complex. This large and tightly regulated multi-protein complex links centromeric chromatin microtubules attached spindle pole body as such leads process. Understanding architecture, function regulation of this vital therefore essential. However, due its complexity dynamics, only individual subcomplexes could be studied in high-resolution structural detail so far. In study we construct a...