A5029640604- Ion channel regulation and function
- Cell Adhesion Molecules Research
- Cardiac electrophysiology and arrhythmias
- Neuroscience and Neuropharmacology Research
- Monoclonal and Polyclonal Antibodies Research
- Cellular Mechanics and Interactions
- Neuroscience and Neural Engineering
- RNA Interference and Gene Delivery
- Nicotinic Acetylcholine Receptors Study
- Ion Transport and Channel Regulation
- 3D Printing in Biomedical Research
- Neuroblastoma Research and Treatments
- Cancer Cells and Metastasis
- Metal complexes synthesis and properties
- Lipid Membrane Structure and Behavior
- Receptor Mechanisms and Signaling
- Cancer, Hypoxia, and Metabolism
- Cancer-related gene regulation
- HER2/EGFR in Cancer Research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Colorectal Cancer Treatments and Studies
- MicroRNA in disease regulation
- Ion Channels and Receptors
- Genetic factors in colorectal cancer
- Ovarian cancer diagnosis and treatment
University of Florence
2016-2025
Tumour Institute of Tuscany
2011-2025
Consorzio Interuniversitario per lo Sviluppo dei Sistemi a Grande Interfase
2020-2023
Istituto Nazionale di Fisica Nucleare, Sezione di Firenze
2015-2018
Azienda Ospedaliero-Universitaria Careggi
2008-2014
University of Leicester
2014
University of Milano-Bicocca
2010-2014
University of Padua
2014
University of Verona
2014
University of Siena
2014
1. Human and murine neuroblastoma cell lines were used to investigate, by the whole-cell patch-clamp technique, properties of a novel inward-rectifying K+ current (IIR) in adjustment resting potential (Vrest), which was range -40 -20 mV. 2. When elicited from holding 0 mV, IIR completely inactivated with time constants ranging 13 ms at -140 mV 4.5 s -50 The steady-state inactivation curve (h(V)) found be independent [Na+]o [K+]o (2-80 mM) could fitted Boltzmann steep slope factor 5-6, V1/2...
Abstract The acquisition of the capacity to invade surrounding tissues confers a more malignant phenotype tumor cells and is necessary for establishment metastases. understanding molecular mechanisms underlying cell invasion in human solid tumors such as colorectal cancers could provide not only sensitive prognostic analyses but also novel targets cancer therapy. We report this article that K+ ion channels belonging HERG family are important determinants an invasive cancers. herg1 gene HERG1...
The solution behavior of auranofin, Et3PAuCl and Et3PAuI, as well their interactions with hen egg white lysozyme, single strand oligonucleotide, ds-DNA were comparatively analyzed through NMR spectroscopy, ESI-MS, ethidium bromide displacement, DNA melting viscometric tests. cytotoxic effects toward representative colorectal cancer cell lines found to be strong similar in the three cases a good correlation could established between cytotoxicity ability inhibit thioredoxin reductase;...
5-Fluorouracil (5-FU) is a key component of chemotherapy for colorectal cancer (CRC). 5-FU efficacy established by intracellular levels folate cofactors and DNA damage repair strategies. However, drug resistance still represents major challenge. Here, we report that alterations in serine metabolism affect sensitivity vitro vivo CRC models. In particular, 5-FU-resistant cells display strong dependency achieved either upregulating endogenous synthesis or increasing exogenous uptake....
Mounting evidence underline the relevance of macromolecular complexes in cancer. Integrins frequently recruit ion channels and transporters within which behave as signaling hubs. A complex composed by β1 integrin, hERG1 K+ channel, neonatal form Na+ channel NaV 1.5 (nNaV1.5) Na+/H+ antiporter NHE1 (NHE1/hERG1/β1/nNaV1.5 complex) has been recently described to be expressed regulate relevant cancer related behaviors Breast Cancer (BCa) cells. We analyzed expression impact on outcome genes...
The regular firing of a Hodgkin‐Huxley neurone endowed with fast Na + and delayed K channels can be converted into adapting by appending HERG (human eag ‐related gene) channels. computer model predictions were verified studying the properties F‐11 DRG x neuroblastoma hybrid cells induced to differentiate long‐term exposure retinoic acid. These cells, which express currents ( I ), show clear spike‐frequency adaptation their when current clamped long depolarizations. In agreement prediction,...
1. Pharmacological blockade of the Human ether-a-go-go related gene (HERG) potassium channel is commonly linked with acquired long QT syndrome and associated proarrhythmia. The objectives this study were (i) to identify characterise any inhibitory action on HERG selective-serotonin re-uptake inhibitor fluvoxamine, (ii) then determine whether fluvoxamine shared consensus molecular determinants those drugs so far tested. 2. Heterologous current (I(HERG)) was measured at 37 degrees C, using...
HERG K+channels, besides contributing to regulate cardiac and neuronal excitability, are preferentially expressed in tumour cell lines of different histogenesis, where their role the development maintenance neoplastic phenotype is under study. We show here that both herg gene protein with high frequency primary human endometrial cancers, as compared normal hyperplastic endometrium. RT-PCR immunohistochemistry, using specific anti-HERG antibodies developed our laboratory, were applied tissue...
Electrical signals elicited by integrin interaction with ECM components and their role in neurite outgrowth were studied two clones (N1 N7) isolated from 41A3 murine neuroblastoma cell line. Although the similarly adhered to fibronectin (FN) vitronectin (VN), this adhesion induced N1 but not N7 cells. Patch clamp recordings whole configuration showed that, upon FN or VN platelet factor 4 (PF4), cells undergo a marked (approximately equal 20 mV) hyperpolarization of resting potential (Vrest)...
Adhesive receptors of the integrin family are primarily involved in cell-extracellular matrix adhesion. Additionally, integrins trigger multiple signaling pathways that cell migration, proliferation, survival, and differentiation. We previously demonstrated activation containing beta(1) subunit leads to a selective increase potassium currents carried by human ether-a-go-go-related gene (hERG) channels neuroblastoma leukemia cells; this current modulates adhesion-dependent differentiation...
BackgroundComplete surgical resection of gastric cancer is potentially curative, but long-term survival poor.
Increasing evidence indicates that ion channels are involved in the pathophysiology of cancer. The human ether-á-go-go-related gene (hERG) can be considered one most critical ion-channel encoding genes establishment and maintenance neoplastic growth. In this review, is presented to demonstrate hERG frequently over- and/or mis-expressed many tumour cell lines as well primary cancers. Moreover, cells, especially leukaemia express a truncated isoform (hERG1B) along with full length hERG1...
Whether hERG1 potassium channels promote proliferation or metastasis in breast cancer cells depends on channel conformation.
A subset of human hepatocellular carcinomas (HCC) exhibit mutations β-catenin gene CTNNB1 and overexpress Glutamine synthetase (GS). The CTNNB1-mutated HCC cell line HepG2 is sensitive to glutamine starvation induced in vitro with the antileukemic drug Crisantaspase GS inhibitor methionine-L-sulfoximine (MSO). Immunodeficient mice subcutaneous xenografts lines HC-AFW1 were treated and/or MSO, tumour growth was monitored. At end treatment, weight histology assessed. Serum tissue amino acids...