A5090926958- Liver Disease Diagnosis and Treatment
- Heat shock proteins research
- Cancer Mechanisms and Therapy
- Liver physiology and pathology
- Protein Kinase Regulation and GTPase Signaling
- Cancer, Hypoxia, and Metabolism
- Melanoma and MAPK Pathways
- Cholangiocarcinoma and Gallbladder Cancer Studies
- FOXO transcription factor regulation
- Phagocytosis and Immune Regulation
- Endoplasmic Reticulum Stress and Disease
- PI3K/AKT/mTOR signaling in cancer
- Autophagy in Disease and Therapy
- Hepatitis C virus research
- Multiple Myeloma Research and Treatments
- Hepatocellular Carcinoma Treatment and Prognosis
- Pancreatitis Pathology and Treatment
- Cytokine Signaling Pathways and Interactions
- Research on Leishmaniasis Studies
- Cancer, Lipids, and Metabolism
- Angiogenesis and VEGF in Cancer
- Peptidase Inhibition and Analysis
- Diet, Metabolism, and Disease
- Cancer Cells and Metastasis
- Chronic Lymphocytic Leukemia Research
University of Florence
2014-2023
Veneto Nanotech (Italy)
2022
Veneto Institute of Molecular Medicine
2004-2012
University of Padua
2002-2012
Magna Graecia University
2012
University of Bonn
2011
Neuroscience Institute
2007-2009
Istituto Superiore di Sanità
2007
Fondazione Humanitas per la Ricerca
1986-1996
Azienda Socio Sanitaria Territoriale degli Spedali Civili di Brescia
1989-1993
CK2 (casein kinase 2) is a very pleiotropic serine/threonine protein whose abnormally high constitutive activity has often been correlated to pathological conditions with special reference neoplasia. The two most widely used cell permeable inhibitors, TBB (4,5,6,7-tetrabromo-1H-benzotriazole) and DMAT (2-dimethylamino-4,5,6,7-tetrabromo-1H-benzimidazole), are marketed as quite specific blockers. In the present study we show, by using panel of approx. 80 kinases, that its parent compound TBI...
Emodin (1,3,8-trihydroxy-6-methyl-anthraquinone) is a moderately potent and poorly selective inhibitor of protein kinase CK2, one the most pleiotropic serine/threonine kinases, implicated in neoplasia other global diseases. By virtual screening MMS (Molecular Modeling Section) database, we have now identified quinalizarin (1,2,5,8-tetrahydroxyanthraquinone) as an CK2 that more than emodin. inhibition by competitive with respect to ATP, Ki value approx. 50 nM. Tested at 1 microM concentration...
Abnormally high constitutive activity of protein kinase CK2, levels which are elevated in a variety tumours, is suspected to underlie its pathogenic potential. The most widely employed CK2 inhibitor 4,5,6,7-tetrabromobenzotriazole (TBB), exhibits comparable efficacy toward another kinase, DYRK1 a. Here we describe the development new class inhibitors, conceptually derived from TBB, have lost their potency In particular, tetrabromocinnamic acid (TBCA) inhibits five times more efficiently than...
Abstract Purpose: Protein kinase CK2 promotes multiple myeloma cell growth by regulating critical signaling pathways. also modulates proper HSP90-dependent client protein folding and maturation phosphorylating its co-chaperone CDC37. Because the endoplasmic reticulum (ER) stress/unfolded response (UPR) is central in pathogenesis, we tested hypothesis that CK2/CDC37/HSP90 axis could be involved UPR cells. Experimental Design: We analyzed activity upon ER stress, effects of inactivation on...
Activation of Kupffer cells and recruitment monocytes are key events in fibrogenesis. These release soluble mediators which induce the activation hepatic stellate (HSCs), main fibrogenic cell type within liver. Mer tyrosine kinase (MerTK) signaling regulates multiple processes macrophages has been implicated pathogenesis non-alcoholic steatohepatitis-related fibrosis. In this study, we explored if MerTK influences profibrogenic phenotype HSCs.Macrophages were derived from THP-1 or...
<h3>Objective</h3> The extracellular signal-regulated kinase 5 (ERK5 or BMK1) is involved in tumour development. ERK5 gene may be amplified hepatocellular carcinoma (HCC), but its biological role has not been clarified. In this study, we explored the of expression and activity HCC vitro vivo. <h3>Design</h3> was evaluated human liver tissue. Cultured HepG2 Huh-7 were studied after knockdown by siRNA presence specific pharmacological inhibitor, XMD8-92. vivo assessed using mouse xenografts....
Oncostatin M (OSM) is a pleiotropic cytokine of the interleukin (IL)-6 family that contributes to progression chronic liver disease. Here we investigated role OSM in development and hepatocellular carcinoma (HCC) non-alcoholic fatty disease (NAFLD)/non-alcoholic steatohepatitis (NASH). The was (1) selected cohorts NAFLD/NASH HCC patients, (2) cancer cells exposed human recombinant or stably transfected overexpress OSM, (3) murine xenografts, (4) NASH-related model hepatic carcinogenesis....
Abstract Cholangiocarcinoma (CCA) is a particularly aggressive hepatobiliary malignancy, for which the molecular mechanisms underlying malignant phenotype are still poorly understood, and novel effective therapeutic strategies limited. The pro-survival protein kinase CK2 frequently overexpressed in cancer receiving increasing interest as an anti-tumor drug target. Its precise role CCA biology largely unknown. Here we show that expression of CK2α α’ catalytic subunits β regulatory subunit...
I-MIBG and 99Tcm(V)-DMSA have been recently proposed as scintigraphic markers of the medullary thyroid carcinoma (MTC). Thirty two patients were examined with 131I- or 123I-MIBG 26 these re-examined (planar SPECT). From our experience we can draw following conclusions: (1) attempt was useless in normal levels plasma calcitonin (CT): (2) high CT levels, sensitivity MIBG better familiar (3/3 true positive) than sporadic disease (7/21 positive; overall = 42%); positive 16/19 cases, (overall...
Background and Aims Cholangiocarcinoma (CCA) is characterized by high resistance to chemotherapy poor prognosis. Several oncogenic pathways converge on activation of extracellular signal‐regulated kinase 5 (ERK5), whose role in CCA has not been explored. The aim this study was investigate the ERK5 biology CCA. Approach Results expression detected two established (HuCCT‐1 CCLP‐1) primary human intrahepatic cell lines (iCCA58 iCCA60). phosphorylation increased cells exposed soluble mediators....
Phosphorylation by protein tyrosine kinases is crucial to the control of growth and development multicellular eukaryotes, including humans, it also seems play an important role in prokaryotes. A plant tyrosine-specific kinase has not been identified yet; hence, plants have suggested share with unicellular eukaryote yeast a phosphorylation system where limited number stress proteins are tyrosyl-phosphorylated only few dual-specificity (serine/threonine tyrosine) kinases. However, preliminary...
Phosphorylation by protein tyrosine kinases is crucial to the control of growth and development multicellular eukaryotes, including humans, it also seems play an important role in prokaryotes. A plant tyrosine-specific kinase has not been identified yet; hence, plants have suggested share with unicellular eukaryote yeast a phosphorylation system where limited number stress proteins are tyrosyl-phosphorylated only few dual-specificity (serine/threonine tyrosine) kinases. However, preliminary...