A5006408383- Liver Disease Diagnosis and Treatment
- Alcohol Consumption and Health Effects
- Drug-Induced Hepatotoxicity and Protection
- Liver Disease and Transplantation
- Free Radicals and Antioxidants
- Electron Spin Resonance Studies
- Diet, Metabolism, and Disease
- Antioxidant Activity and Oxidative Stress
- Pharmacogenetics and Drug Metabolism
- Organ Transplantation Techniques and Outcomes
- Diabetes and associated disorders
- Drug Transport and Resistance Mechanisms
- Liver physiology and pathology
- Eicosanoids and Hypertension Pharmacology
- Hepatitis C virus research
- Cancer, Hypoxia, and Metabolism
- Liver Diseases and Immunity
- Inflammatory Bowel Disease
- Genomics, phytochemicals, and oxidative stress
- Sulfur Compounds in Biology
- Vitamin C and Antioxidants Research
- Cardiac Ischemia and Reperfusion
- Adenosine and Purinergic Signaling
- Microscopic Colitis
- Atherosclerosis and Cardiovascular Diseases
Università degli Studi del Piemonte Orientale “Amedeo Avogadro”
2015-2025
University of Pisa
2017-2020
University of Turin
1992-2015
Piedmont University
2008-2014
Newcastle University
2013
Institute of Cancer Research
2013
Electron (Ukraine)
2013
University of East
1999-2011
University of the East
2002-2009
Brown University
2009
Previous studies have shown that human nonalcoholic steatohepatitis (NASH) is often associated with the presence of circulating antibodies against protein adducted by lipid peroxidation products. Here we used methionine-choline deficient (MCD) model NASH to characterize possible involvement adaptive immunity in NASH. In mice fed up 8 weeks MCD diet extension liver injury and lobular inflammation paralleled development immunoglobulin G (IgG) malonyldialdehyde (MDA) 4-hydroxynonenal...
N-Acetyl-p-benzoquinone imine (NAPQI), a reactive metabolite of acetaminophen, rapidly reacts at physiological pH with glutathione (GSH) forming an acetaminophen-glutathione conjugate and stoichiometric amounts acetaminophen disulfide (GSSG). The same reaction products are formed in isolated hepatocytes incubated NAPQI. In which have been treated 1,3-bis-(2-chloroethyl)-1-nitrosourea (BCNU) order to inhibit reductase, the initial rise GSSG concentration presence NAPQI is maintained, whereas...
We have previously shown that the treatment with diallyl sulfide (DAS) and phenylethyl isothiocyanate (PIC) of rats receiving ethanol in alcohol tube-feeding model effectively suppressed induction cytochrome P4502E1 (CYP2E1) by ethanol. Here we report rat DAS PIC significantly decreased trapping hydroxyethyl free radicals liver microsomes incubated vitro Furthermore, these inhibitors also greatly reduced production radical-derived epitopes detectable vivo ethanol-fed rats. The action on...
ABSTRACT Previous studies have shown that α–tocopherol (vitamin E) pretreatment of experimental animals can protect against acute liver necrosis induced by carbon tetrachloride. In this study we investigated whether the increase vitamin E content dietary supplementation influences chronic damage and cirrhosis tetrachloride in rat. Our data indicate did not interfere with growth rate increased about threefold liver's vitamin. Vitamin significantly reduced oxidative damage, but it was...
<b>Aims:</b> Factors responsible for the progression of non-alcoholic fatty liver disease (NAFLD) to more severe injury are poorly understood. In present study, we investigated association between immune reactions triggered by oxidative stress and stage NAFLD. <b>Methods:</b> Titres IgG against human serum albumin adducted with malondialdehyde (MDA-HSA) or arachidonic acid hydroperoxide (AAHP) oxidised cardiolipin (Ox-CL) were measured in 167 NAFLD patients steatosis only (n = 79),...
1. The metabolic activation of carbon tetrachloride to free-radical intermediates is an important step in the sequence disturbances leading acute liver injury produced by this toxic agent. Electron-spin-resonance (e.s.r.) spin-trapping techniques were used characterize species involved. 2. Spin trapping was applied microsomal fractions presence NADPH, and isolated intact rat hepatocytes. results obtained with spin trap N-benzylidene-2-methylpropylamine N-oxide (‘phenyl t-butyl nitrone’)...
The inclusion of uric acid in the incubation medium during copper-induced low-density lipoprotein (LDL) oxidation exerted either an antioxidant or pro-oxidant effect. effect, as mirrored by enhanced formation conjugated dienes, lipid peroxides, thiobarbituric acid-reactive substances and increase negative charge, occurred when was added late inhibitory lag phase subsequent extensive propagation copper-stimulated LDL oxidation. effect specific for required presence copper Cu(I) Cu(II). In...
This study was done to determine if a relationship exists between CYP2E1 induction by ethanol, lipid peroxidation, and liver pathology in experimental alcohol-induced disease the rat. Rats were fed ethanol with or without diallyl sulfide (DAS) phenethyl isothiocyanate (PIC) intragastrically for 1 month. correlated microsomal hydroxylation of paranitrophenol, score using data from PIC-fed rats. Some DAS-fed rats not included here because they have been reported elsewhere. Microsomal protein...
Acetaldehyde and malonildialdehyde can form hybrid protein adducts, named MAA adducts that have strong immunogenic properties. The formation of in the liver chronic alcohol-fed rats is associated with development circulating antibodies specifically recognized these adducts. aim this study was to examine whether might participate immune response human alcohol-induced disease. Circulating against were evaluated 50 patients hepatitis or cirrhosis, 40 non-alcohol-induced disease, 15 heavy...
Ethanol-inducible CYP2E1 is an enzyme of major toxicological interest because it metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. has also been implicated in alcohol liver disease its contribution oxidative stress. Previously, polymorphic alleles with mutations introns the 5'-flanking regulatory region have described, their presence related incidence lung cancer. In present investigation, we investigated whether any functional are linked above-mentioned rare...