A5010039762- Protein Kinase Regulation and GTPase Signaling
- Cancer, Hypoxia, and Metabolism
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Metabolism, Diabetes, and Cancer
- Pancreatic function and diabetes
- Fungal and yeast genetics research
- Metabolomics and Mass Spectrometry Studies
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Cardiac, Anesthesia and Surgical Outcomes
- Cancer, Lipids, and Metabolism
- PI3K/AKT/mTOR signaling in cancer
- Cancer-related Molecular Pathways
- Peptidase Inhibition and Analysis
- Biochemical and Molecular Research
- Diabetes Treatment and Management
- Cancer-related molecular mechanisms research
- Microbial Metabolic Engineering and Bioproduction
- Ion channel regulation and function
- Bioinformatics and Genomic Networks
- 3D Printing in Biomedical Research
- Melanoma and MAPK Pathways
- Cardiomyopathy and Myosin Studies
- Cancer Cells and Metastasis
- Innovative Microfluidic and Catalytic Techniques Innovation
University of Milano-Bicocca
2016-2025
University of Milan
2006-2024
Milano Metropoli Development Agency
2012
Weatherford College
2012
National Research Council
2010
Politecnico di Milano
2010
Harvard University
1991
// Corti Denise 1, 2, * , Paolo Paoli Maura Calvani 1 Maria Letizia Taddei Elisa Giannoni Scott Kopetz 2 Syed Mohammad Ali Kazmi Morelli Pia Piergiorgio Pettazzoni Elena Sacco 3 Anna Caselli Marco Vanoni Matteo Landriscina 4 Cirri Paola Chiarugi Department of Experimental and Clinical Biomedical Sciences, University Florence, Italy Genomic Medicine, The Texas MD Anderson Cancer Center, Houston, Texas, USA SYSBIO Centre for Systems Biology, Biotechnology Biosciences, Milano-Bicocca, Milano,...
Abstract Modeling human neuronal properties in physiological and pathological conditions is essential to identify novel potential drugs explore mechanisms of neurological diseases. For this purpose, we generated a three-dimensional (3D) culture, by employing the readily available neuroblastoma SH-SY5Y cell line, new differentiation protocol. The entire process occurred matrix lasted 47 days, with 7 days pre-differentiation phase 40 differentiation, allowed development 3D culture consistent...
Abstract Aims The heterozygous phospholamban (PLN) mutation R14del (PLN +/− ) is associated with a severe arrhythmogenic cardiomyopathy (ACM) developing in the adult. “Superinhibition” of SERCA2a by PLN widely assumed to underlie pathogenesis, but alternative mechanisms such abnormal energy metabolism have also been reported. This work aims (1) evaluate Ca 2+ dynamics and transgenic (TG) mouse model prior development; (2) test whether they are causally connected. Methods dynamics,...
Recent epidemiological studies have demonstrated that the consumption of healthy foods are particularly rich in polyphenols might reduce incidence cancer and neurodegenerative diseases. In particular, chlorogenic acids (CGAs) occur ubiquitously food represent most abundant human diet. A number beneficial biological effects CGAs, such as anti-inflammatory activity, anti-carcinogenic protection against diseases, been reported. However, molecular mechanisms at base these activities not yet...
Three-dimensional cancer models, such as spheroids, are increasingly being used to study metabolism because they can better recapitulate the molecular and physiological aspects of tumor architecture than conventional monolayer cultures. Although Agilent Seahorse XFe96 (Agilent Technologies, Santa Clara, CA, United States) is a valuable technology for studying metabolic alterations occurring in cells, its application three-dimensional cultures still poorly optimized. We present reliable...
Abstract Background Glucagon like peptide-1 receptor agonists (GLP-1RAs) have shown to reduce mortality and cardiovascular events in patients with type 2 diabetes mellitus (T2DM). Since the impairment number function of vasculotrophic circulating CD34 + hematopoietic stem progenitor cells (HSPCs) T2D has been reported increase (CV) risk, we hypothesized that one mechanisms whereby GLP-1 RAs exert CV protective effects may be related ability improve HSPC function. Methods In cord blood...
Cannabidiol (CBD) is a non-psychoactive phytocannabinoid that has been discussed for its safety and efficacy in cancer treatments. For this reason, we have inquired into use on triple-negative human breast cancer. Analyzing the biological effects of CBD MDA-MB-231, demonstrated both dosage serum concentrations culture medium influence outcomes; furthermore, light scattering studies impacts aggregation state by acting as surfactant agent. Pharmacological combination with chemotherapeutic...
Exopolymers from a diverse collection of marine and freshwater bacteria were characterized by pyrolysis-mass spectrometry (Py-MS). Py-MS provides spectra pyrolysis fragments that are characteristic the original material. Analysis multivariate statistical techniques (principal component canonical variate analysis) separated these exopolymers into distinct groups. clearly distinguished fragments, which may be derived components responsible for functional differences between polymers. The...
Cocoa beans are one of the largest cultivated crops all over world, producing large amount by-products. For this reason, it is necessary to valorise cocoa by-products obtain valuable source bioactive compounds. In paper, a pressurized hot water extraction process for recovery theobromine and caffeine from by-product was developed optimized. The carried out on ASE parameters affected by efficiency antioxidant capacity were optimized chemometric approach. Theobromine quantified UPLC-UV (283...
Cell growth and proliferation require a complex series of tight-regulated well-orchestrated events. Accordingly, proteins governing such events are evolutionary conserved, even among distant organisms. By contrast, it is more singular the case "core functions" exerted by functional analogous that not homologous do share any kind structural similarity. This regulating G1/S transition in higher eukaryotes-i.e., retinoblastoma (Rb) tumor suppressor Rb-and budding yeast, i.e., Whi5. The...
Bladder cancer is one of the most prevalent deadly diseases worldwide. Grade 2 tumors represent a good window therapeutic intervention, whose optimization requires high resolution biomarker identification. Here we characterize energy metabolism and cellular properties associated with spreading tumor progression RT112 5637, two cell lines derived from human bladder, representative luminal-like basal-like tumors, respectively. The have similar proliferation rates, but only 5637 cells show...
RalGPS2 is a Ras-independent Guanine Nucleotide Exchange Factor for RalA GTPase that involved in several cellular processes, including cytoskeletal organization. Previously, we demonstrated also plays role the formation of tunneling nanotubes (TNTs) bladder cancer 5637 cells. In particular, TNTs are novel mechanism cell-cell communication tumor microenvironment, playing central progression and metastasis formation. However, molecular mechanisms still need to be fully elucidated. Here...
Since mutations of Ras genes have a great incidence in human tumours, oncoproteins are major clinical target for the development anticancer agents. We developed synthetic molecules able to inhibit activation. Here we present new, water-soluble inhibitors composed by an aromatic pharmacofore moiety covalently linked different sugars. New glycosylated compounds bind Switch 2 region Ras, also involved effector binding, GEF-catalyzed nucleotide exchange on vitro, and reduce Ras-dependent...