A5043790154- Angiogenesis and VEGF in Cancer
- Epigenetics and DNA Methylation
- Immune cells in cancer
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Pancreatic function and diabetes
- Electrospun Nanofibers in Biomedical Applications
- Cancer, Hypoxia, and Metabolism
- Cancer Cells and Metastasis
- Sphingolipid Metabolism and Signaling
- Liver Diseases and Immunity
- Nitric Oxide and Endothelin Effects
- Eicosanoids and Hypertension Pharmacology
- MicroRNA in disease regulation
- Liver Disease Diagnosis and Treatment
- Atherosclerosis and Cardiovascular Diseases
- Medical Imaging Techniques and Applications
- Congenital heart defects research
- Pharmacological Receptor Mechanisms and Effects
- Cannabis and Cannabinoid Research
- Pain Mechanisms and Treatments
- Histone Deacetylase Inhibitors Research
- Diabetes Treatment and Management
- Metabolism, Diabetes, and Cancer
- Cell death mechanisms and regulation
Centro Cardiologico Monzino
2015-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2016-2025
Azienda Socio Sanitaria Territoriale Grande Ospedale Metropolitano Niguarda
2021
Institute of Cancer Research
2019
Institute of Cancer Research
2015-2016
Alzheimer’s Disease Neuroimaging Initiative
2015
University of Michigan–Ann Arbor
2015
Pfizer (United States)
2015
Brigham and Women's Hospital
2015
University of Pittsburgh
2015
Melanoma is one of the most aggressive types human cancer, characterized by enhanced heterogeneity and resistance to conventional therapy at advanced stages. We others have previously shown that HEDGEHOG-GLI (HH-GLI) signaling required for melanoma growth survival expansion melanoma-initiating cells (MICs). Recent reports indicate HH-GLI regulates a set genes typically expressed in embryonic stem cells, including SOX2 (sex-determining region Y (SRY)-Box2). Here we address function melanomas...
Abstract Purpose: Cannabinoids have been recently proposed as a new family of potential antitumor agents. The present study was undertaken to investigate the expression two cannabinoid receptors, CB1 and CB2, in colorectal cancer provide insight into molecular pathways underlying apoptotic activity induced by their activation. Experimental Design: Cannabinoid receptor investigated both human specimens DLD-1 HT29 colon cell lines. effects agonist arachinodyl-2'-chloroethylamide CB2...
It is common knowledge that platelet-derived growth factor (PDGF) a critical regulator of mesenchymal cell migration and proliferation. Nevertheless, these two cellular responses are mutually exclusive. To solve this apparent contradiction, we studied the behavior NIH3T3 fibroblasts in response to increasing concentrations PDGF. We found there strong proliferation induction only with PDGF >5 ng/ml, whereas arises starting from 1 ng/ml negligible at higher concentrations. According phenotypic...
The question of whether cancer stem/tumor-initiating cells (CSC/TIC) exist in human melanomas has arisen the last few years. Here, we have used nonadherent spheres and aldehyde dehydrogenase (ALDH) enzymatic activity to enrich for CSC/TIC a collection obtained from broad spectrum sites stages. We find that melanomaspheres display extensive vitro self-renewal ability sustain tumor growth vivo, generating melanoma xenografts recapitulate phenotypic composition parental tumor. Melanomaspheres...
Advanced glycation end-products (AGEs) and their interaction with the receptor for advanced (RAGE) play a pivotal role in development progression of type 2 diabetes. In this retrospective cohort study, we explored association circulating levels soluble RAGE (sRAGE) isoforms, i.e., endogenous secretory esRAGE cleaved cRAGE, AGEs respective ratios 15-year all-cause mortality diabetes.Baseline sRAGE isoforms concentration were measured by ELISA 362 patients diabetes 125 age- gender-matched...
Recently, carbonic anhydrase (CA) inhibitors have been proposed as a potential new class of antitumor agents. The aim this study was to evaluate the activity three CA inhibitors, namely acetazolamide (AZ) and two newly synthesized aromatic sulfonamides with high affinity for IX, 2-(4-sulfamoylphenyl-amino)-4,6-dichloro-1,3,5-triazine (TR1) 4-[3-(<i>N</i>,<i>N</i>-dimethylaminopropyl)thioreidophenylsulfonylaminoethyl]benzenesulfonamide (GA15), against human tumor cells. effects AZ, TR1, GA15...
Revascularization strategies failed to improve outcome in diabetic (DM) patients with peripheral artery disease (PAD). Histone modifications are key modulators of gene expression and could play a role angiogenic response. This study investigates the chromatin remodelling modulating angiogenesis DM. RNA sequencing (RNA-seq) assays (cell migration tube formation) were performed human aortic endothelial cells (HAECs) exposed normal glucose (NG, 5 mM) or high (HG, 25 for 48h. The histone...
<p dir="ltr">Revascularization strategies failed to improve outcome in diabetic (DM) patients with peripheral artery disease (PAD). Histone modifications are key modulators of gene expression and could play a role angiogenic response. This study investigates the chromatin remodelling modulating angiogenesis DM. RNA sequencing (RNA-seq) assays (cell migration tube formation) were performed human aortic endothelial cells (HAECs) exposed normal glucose (NG, 5 mM) or high (HG, 25 for 48h....
<p dir="ltr">Revascularization strategies failed to improve outcome in diabetic (DM) patients with peripheral artery disease (PAD). Histone modifications are key modulators of gene expression and could play a role angiogenic response. This study investigates the chromatin remodelling modulating angiogenesis DM. RNA sequencing (RNA-seq) assays (cell migration tube formation) were performed human aortic endothelial cells (HAECs) exposed normal glucose (NG, 5 mM) or high (HG, 25 for 48h....
We report for the first time that penile smooth muscle cells (SMC) not only respond to, but also synthesize, endothelin-1 (ET-1), one of main regulators SMC activity. Immunohistochemical studies indicated that, beside endothelial (EC), human adult and fetal penis express ET-1 its converting enzyme, ECE-1. Accordingly, cultures stromal these genes. prepared characterized from fetuses. These specific markers such as α actin phosphodiesterase type 5A3 along with hallmarks androgen-dependent...
The effective treatment of pain is typically limited by a decrease in the pain-relieving action morphine that follows its chronic administration (tolerance). Therefore, restoring opioid efficacy great clinical importance. In murine model antinociceptive tolerance, repeated significantly stimulated enzymatic activities spinal cord serine palmitoyltransferase, ceramide synthase, and acid sphingomyelinase (enzymes involved de novo pathways biosynthesis, respectively) led to peroxynitrite-derive...
Abstract Patients requiring diagnostic testing for coronavirus disease 2019 (COVID-19) are routinely assessed by reverse-transcription quantitative polymerase chain reaction (RT-qPCR) amplification of Sars-CoV-2 virus RNA extracted from oro/nasopharyngeal swabs. Despite the good specificity assays certified SARS-CoV-2 molecular detection, and a theoretical sensitivity few viral gene copies per reaction, relatively high rate false negatives continues to be reported. This is an important...
Abstract Background Glucagon like peptide-1 receptor agonists (GLP-1RAs) have shown to reduce mortality and cardiovascular events in patients with type 2 diabetes mellitus (T2DM). Since the impairment number function of vasculotrophic circulating CD34 + hematopoietic stem progenitor cells (HSPCs) T2D has been reported increase (CV) risk, we hypothesized that one mechanisms whereby GLP-1 RAs exert CV protective effects may be related ability improve HSPC function. Methods In cord blood...
We have previously shown that hypoxia makes vascular smooth muscle cells (VSMCs) responsive to placental growth factor (PlGF) through the induction of functional fms-like tyrosine kinase (Flt-1) receptors. The aim this study was investigate molecular mechanisms involved in PlGF effects on proliferation and contraction VSMCs exposed (3% O2). In cultured rat hypoxia, increased phosphorylation protein B (Akt), p38 STAT3; activation STAT3 higher than other kinases. agreement with finding,...
Background The pericardial tissue is commonly used to produce bio-prosthetic cardiac valves and patches in surgery. procedures adopted prepare this consist treatment with aldehydes, which do not prevent post-graft calcification due incomplete xeno-antigens removal. adoption of fixative-free decellularization protocols has been therefore suggested overcome limitation. Although promising, the decellularized pericardium yet clinics, absence proofs indicating that cryopreservation can...
Background CD 34+ stem/progenitor cells are involved in vascular homeostasis and neovascularization of ischemic tissues. The number circulating stem is a predictive biomarker adverse cardiovascular outcomes diabetic patients. Here, we provide evidence that hyperglycemia can be "memorized" by the through epigenetic changes contribute to onset maintenance their dysfunction diabetes mellitus. Methods Results Cord-blood-derived exposed high glucose displayed increased reactive oxygen species...
Abstract Background Diabetes-induced trained immunity contributes to the development of atherosclerosis and its complications. This study aimed investigate in humans whether epigenetic signals involved immune cell activation inflammation are initiated hematopoietic stem/progenitor cells (HSPCs) transferred differentiated progeny. Methods results High glucose (HG)-exposure cord blood (CB)-derived HSPCs induced a senescent-associated secretory phenotype (SASP) characterized by proliferation...
Saphenous vein graft disease is a timely problem in coronary artery bypass grafting. Indeed, after exposure of the to arterial blood flow, progressive modification wall begins, due proliferation smooth muscle cells intima. As consequence, progressively occludes and this leads recurrent ischemia. In present study we employed novel ex vivo culture system assess biological effects arterial-like pressure on human saphenous structure physiology, compare results those achieved presence constant...
Abstract Background Recent data demonstrate that endothelin‐1 (ET‐1) concentration increases in plasma of men with advanced, hormone‐refractory prostate adenocarcinoma. In addition, ET‐1 is involved osteblastic remodelling and new bone formation, suggesting a role for this vasoactive peptide the metastatic progression cancer to bone. Methods We investigated regulation expression androgen‐sensitive insensitive cell lines by androgens several factors (EGF) (TGFβ‐1, IL1‐α IGF‐1). Results...