A5017193017- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Aortic aneurysm repair treatments
- Chemokine receptors and signaling
- IL-33, ST2, and ILC Pathways
- Atherosclerosis and Cardiovascular Diseases
- Ferroptosis and cancer prognosis
- Sports injuries and prevention
- Cardiovascular Effects of Exercise
- Aortic Disease and Treatment Approaches
- Gut microbiota and health
- Salivary Gland Disorders and Functions
- Gas Sensing Nanomaterials and Sensors
- Immune cells in cancer
- Transplantation: Methods and Outcomes
- Vascular Malformations and Hemangiomas
- SARS-CoV-2 detection and testing
- Testicular diseases and treatments
- Multiple Myeloma Research and Treatments
- Nuclear Receptors and Signaling
- Oral Health Pathology and Treatment
- SARS-CoV-2 and COVID-19 Research
Ludwig-Maximilians-Universität München
2021-2025
German Centre for Cardiovascular Research
2022-2025
University of Milan
2019-2024
IRCCS Humanitas Research Hospital
2019-2023
Humanitas University
2017-2022
Istituti di Ricovero e Cura a Carattere Scientifico
2015-2022
Centro Cardiologico Monzino
2015-2017
Fondazione IRCCS Istituto Nazionale dei Tumori
1974
microRNAs (miRNAs) modulate gene expression by repressing translation of targeted genes. Previous work has established a role for miRNAs in regulating vascular smooth muscle cell (VSMC) activity. Whether circular RNAs are involved the modulation miRNA activity VSMCs is unknown.We aimed to identify interacting with enriched and modulating cells' activity.RNA sequencing bioinformatics identified several VSMCs; however, only one, possessing multiple putative binding sites miR-145, was highly...
MicroRNAs (miRNAs, miRs) are small noncoding RNAs that modulate gene expression by negatively regulating translation of target genes. Although the role several miRNAs in vascular smooth muscle cells (VSMCs) has been extensively characterized, function miRNA-128-3p (miR-128) is still unknown.To determine if miR-128 modulates VSMC phenotype and to define underlying mechanisms.We screened for whose modulated an altered DNA methylation status VSMCs, among hits, we selected miR-128. We found was...
Arrhythmogenic cardiomyopathy (ACM) is a genetic disorder mainly due to mutations in desmosomal genes, characterized by progressive fibro-adipose replacement of the myocardium, arrhythmias, and sudden death. It still unclear which cell type responsible for substitution molecular mechanisms lead this structural change. Cardiac mesenchymal stromal cells (C-MSC) are most abundant heart, with propensity differentiate into several types, including adipocytes, their role ACM unknown. The aim...
Histone H3 dimethylation at lysine 79 is a key epigenetic mark uniquely induced by methyltransferase disruptor of telomeric silencing 1-like (DOT1L). We aimed to determine whether DOT1L modulates vascular smooth muscle cell (VSMC) phenotype and how it might affect atherosclerosis in vitro vivo, unravelling the related mechanism.Gene expression screening VSMCs stimulated with BB isoform platelet-derived growth factor led us identify Dot1l as an early up-regulated factor. Mouse human...
Adult vascular smooth muscle cells (VSMCs) dedifferentiate in response to extracellular cues such as damage and inflammation. Dedifferentiated VSMCs are proliferative, migratory, less contractile, can contribute repair well cardiovascular pathologies intimal hyperplasia/restenosis coronary artery arterial aneurysm. We here demonstrate the role of ubiquitin-like containing PHD RING finger domains 1 (UHRF1) an epigenetic master regulator VSMC plasticity. UHRF1 expression correlated with...
BACKGROUND: The activation and polarization of T cells play a crucial role in atherosclerosis dictate athero-inflammation. epigenetic enzyme EZH2 (enhancer zeste homolog 2) mediates the H3K27me3 (trimethylation histone H3 lysine 27) is pivotal controlling cell responses. METHODS: To detail atherosclerosis, we used human carotid endarterectomy specimens to reveal plaque expression geography EZH2. Atherosclerosis-prone Apoe (apolipoprotein E)–deficient mice with CD (cluster differentiation) 4...
Diagnosis of Arrhythmogenic CardioMyopathy (ACM) is challenging and often late after disease onset. No circulating biomarkers are available to date. Given their involvement in several cardiovascular diseases, plasma microRNAs warranted investigation as potential non-invasive diagnostic tools ACM. We sought identify differentially expressed ACM with respect Healthy Controls (HC) Idiopathic Ventricular Tachycardia patients (IVT), differential diagnosis. HC subjects were screened for plasmatic...
Abstract Diffuse large B-cell lymphoma (DLBCL) is the most frequent type of non-Hodgkin lymphoma. Despite a favorable therapeutic response to first-line chemo-immunotherapy, still 30–40% patients refractory, or relapse after this treatment. Thus, alternative strategies must be sought. Previous studies have indicated that cyclin-dependent kinase 5 (CDK5), serine/threonine protein kinase, involved in tumor development and progression, it may represent potential target. However, its role...
The SARS-CoV-2 pandemic has overwhelmed the treatment capacity of health care systems during highest viral diffusion rate. Patients reaching emergency department had to be either hospitalized (inpatients) or discharged (outpatients). Still, decision was taken based on individual assessment actual clinical condition, without specific biomarkers predict future improvement deterioration, and patients often returned hospital for aggravation their condition. Here, we have developed a new combined...
The CXC chemokine receptor 4 (CXCR4) in endothelial cells (ECs) and vascular smooth muscle (VSMCs) is crucial for integrity. atheroprotective functions of CXCR4 may be counteracted by atherogenic other nonvascular cell types. Thus, strategies cell-specifically augmenting function are if this to useful as a therapeutic target treating atherosclerosis disorders. Here, we identified miR-206-3p vascular-specific repressor exploited target-site blocker (CXCR4-TSB) that disrupted the interaction...
Proteolytic processing of CXCL5 potentiates its chemoattractant activity toward neutrophils and monocytes in vitro vivo.
Since 1961, 285 cases of testicular neoplasms have been investigated by means lymphangiography at the National Cancer Institute Milan. Bilateral foot was performed in 233 cases, 46 patients had combined funicular and lymphangiography, 6 only performed. Lymphangiograms were probative for secondaries retroperitoneal lymph-nodes 162 (56.8 %). The most frequent site metastasis para-aortic nodes (154 cases) with bilateral involvement 68. Funicular lymphangiograms showed primary lymph-centre 28/52...