A5053633424- Biomarkers in Disease Mechanisms
- Healthcare Systems and Public Health
- Orthopedic Infections and Treatments
- Inflammasome and immune disorders
- Lymphatic System and Diseases
- Orthopaedic implants and arthroplasty
- Gut microbiota and health
- Oral microbiology and periodontitis research
- Respiratory viral infections research
- Respiratory Support and Mechanisms
- Total Knee Arthroplasty Outcomes
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Bioinformatics and Genomic Networks
- Systemic Lupus Erythematosus Research
- Digestive system and related health
- Advanced Glycation End Products research
- SARS-CoV-2 and COVID-19 Research
- Phagocytosis and Immune Regulation
- Computational Drug Discovery Methods
- SARS-CoV-2 detection and testing
- Kruppel-like factors research
- Sepsis Diagnosis and Treatment
- MicroRNA in disease regulation
- Bone health and osteoporosis research
- Circular RNAs in diseases
IRCCS Humanitas Research Hospital
2011-2024
Humanitas University
2013-2020
Istituti di Ricovero e Cura a Carattere Scientifico
2015-2019
University of Turin
2013
Fondazione Humanitas per la Ricerca
2006-2010
University of Crete
2009
Justus-Liebig-Universität Gießen
2009
Friedrich-Alexander-Universität Erlangen-Nürnberg
2009
Pentraxin 3 (PTX3) is a fluid-phase pattern recognition molecule and key component of the humoral arm innate immunity. In four different models tissue damage in mice, PTX3 deficiency was associated with increased fibrin deposition persistence, thicker clots, followed by collagen deposition, when compared controls. Ptx3-deficient macrophages showed defective pericellular fibrinolysis vitro. PTX3-bound fibrinogen/fibrin plasminogen at acidic pH plasmin-mediated fibrinolysis. The second...
Abstract The protopypic long pentraxin 3 (PTX3) is a unique, humoral pattern-recognition receptor, which plays nonredundant function in innate resistance to pathogens. Dendritic cells (DC) of myelomonocytic origin, but not plasmacytoid DC, are major source PTX3 response Toll-like receptor (TLR) engagment. present study was designed explore the regulation production DC. induced by TLR ligands, CD40 ligand, and interleukin (IL)-1β suppressed dexamethasone, 1α, 25-dihydroxivitamin D3,...
Abstract Chronic lung infections by Pseudomonas aeruginosa strains are a major cause of morbidity and mortality in cystic fibrosis (CF) patients. Although there is no clear evidence for primary defect the immune system CF patients, host generally unable to P. from airways. PTX3 soluble pattern recognition receptor that plays nonredundant roles innate response fungi, bacteria, viruses. In particular, deficiency associated with increased susceptibility infection. To address potential...
Article5 November 2018Open Access Source DataTransparent process Pentraxin 3 regulates synaptic function by inducing AMPA receptor clustering via ECM remodeling and β1-integrin Giuliana Fossati Humanitas Clinical Research Center - IRCCS, Rozzano, Milano, Italy Search for more papers this author Davide Pozzi Department of Biomedical Sciences, University, Milan, Alice Canzi Filippo Mirabella Sonia Valentino Raffaella Morini Elsa Ghirardini Dipartimento di Biotecnologie Mediche e Medicina...
PTX3 is an inflammatory mediator acting as a fluid phase pattern recognition molecule and playing essential role in innate immunity matrix remodeling. Inflammatory mediators also contribute to skeletal homeostasis, operating at multiple levels physiological pathological conditions. The present study was designed investigate the of remodeling bone healing. Micro-computed tomography (CT) histomorphometry distal femur showed that gene-targeted female male mice (ptx3-/-) had lower trabecular...
The SARS-CoV-2 pandemic has overwhelmed the treatment capacity of health care systems during highest viral diffusion rate. Patients reaching emergency department had to be either hospitalized (inpatients) or discharged (outpatients). Still, decision was taken based on individual assessment actual clinical condition, without specific biomarkers predict future improvement deterioration, and patients often returned hospital for aggravation their condition. Here, we have developed a new combined...
Long pentraxin 3 (PTX3) is a non-redundant component of the humoral arm innate immunity. The present study was designed to investigate interaction PTX3 with Neisseria meningitidis. bound acapsular meningococcus, Neisseria-derived outer membrane vesicles (OMV) and selected meningococcal antigens (GNA0667, GNA1030 GNA2091). PTX3-recognized microbial moieties are conserved structures which fulfil essential functions. Ptx3-deficient mice had lower antibody response in vaccination protocols OMV...
Periprosthetic joint infections (PJIs) are serious complications of prosthetic surgery. The criteria for the diagnosis PJI integrate clinical and laboratory findings in a complex sometimes inconclusive workflow. Host immune factors hold potential as diagnostic biomarkers bone infections. We reported that humoral pattern-recognition molecule long pentraxin 3 (PTX3) predicts total hip knee arthroplasty (THA TKA, respectively). If how genetic variation PTX3 inflammatory genes affect its...
Background Early prognostic stratification of patients with sepsis is a difficult clinical challenge. Aim this study was to evaluate novel molecules in association parameters as predictors 90-days mortality admitted at Humanitas Research Hospital. Methods Plasma samples were collected from 178 patients, diagnosed based on Sepsis-3 criteria, admission the Emergency Department and after 5 days hospitalization. Levels pentraxin 3 (PTX3), soluble IL-1 type 2 receptor (sIL-1R2), panel pro-...
Streptococcus pneumoniae is a major pathogen in children, elderly subjects, and immunodeficient patients. Pentraxin 3 (PTX3) fluid-phase pattern recognition molecule (PRM) involved resistance to selected microbial agents regulation of inflammation. The present study was designed assess the role PTX3 invasive pneumococcal infection. In murine model infection, strongly induced non-hematopoietic (particularly, endothelial) cells. IL-1β/MyD88 axis played Ptx3 gene expression. −/− mice presented...
Acute lung injury and acute respiratory distress syndrome (ALI/ARDS) are severe forms of bilateral inflammation with poor clinical outcomes. However, the pathophysiology ALI/ARDS remains largely obscure. Soluble receptor for advanced glycation endproducts (sRAGE) plays a key regulatory role during phase inflammation, baseline plasma levels sRAGE were recently found to be associated severity ALI/ARDS. We analyzed, in patients, alveolar over time association injury. enrolled 21 patients...
A new immunoassay based on surface plasmon resonance (SPR) for the rapid, reproducible and sensitive determination of pentraxin-3 (PTX3) levels in human plasma has been developed characterized. The method involves a 3-min flow over sensor chip pre-coated with monoclonal anti-PTX3 antibody (MNB4), followed by polyclonal (pAb), required specific recognition captured PTX3. SPR signal generated this secondary linearly correlates PTX3 concentration, range 5–1500 ng/mL, lowest limit detection 5...
Background: Pentraxin3 (PTX3) is overexpressed in kidneys of patients developing lupus nephritis (LN). Active LN associated with reduced anti-PTX3 antibodies. However, abnormalities B cell differentiation against PTX3 have not been characterized systemic erythematosus (SLE). Objective: Characterization PTX3-specific (PTX3+) cells peripheral blood SLE or without and healthy donors (HD). Patients Methods: LN, biopsy-proven matched HD were analyzed. was defined as proteinuria>0.5 g/day CrCl<60...
Summary The long pentraxin 3 (PTX3) is a multifunctional soluble pattern recognition receptor, involved in several processes ranging from innate resistance and inflammation to clearance of apoptotic cells organization hyaluronic acid‐rich extracellular matrices. PTX3 also novel marker pathological conditions infectious, inflammatory, or autoimmune origin. This study was designed assess whether expressed the male reproductive tract interacts with human spermatozoa influencing their function....