A5103104148- Chemokine receptors and signaling
- Immune Response and Inflammation
- Cell Adhesion Molecules Research
- Glycosylation and Glycoproteins Research
- Peptidase Inhibition and Analysis
- Immunotherapy and Immune Responses
- Protease and Inhibitor Mechanisms
- Silicon and Solar Cell Technologies
- Monoclonal and Polyclonal Antibodies Research
- Thin-Film Transistor Technologies
- Nanocluster Synthesis and Applications
- Proteoglycans and glycosaminoglycans research
- biodegradable polymer synthesis and properties
- Animal Behavior and Reproduction
- Polyoxometalates: Synthesis and Applications
- Semiconductor materials and interfaces
- Drug-Induced Hepatotoxicity and Protection
- interferon and immune responses
- Multiple Myeloma Research and Treatments
- Silicon Nanostructures and Photoluminescence
- Cytokine Signaling Pathways and Interactions
- Gout, Hyperuricemia, Uric Acid
- Veterinary medicine and infectious diseases
- Autoimmune and Inflammatory Disorders Research
- Plant and animal studies
Rega Institute for Medical Research
2015-2021
KU Leuven
1981-2021
Universidade Federal de Minas Gerais
2016-2021
Abstract SDS‐PAGE/Edman degradation and HPLC MS/MS showed that zirconium(IV)‐substituted Lindqvist‐, Keggin‐, Wells–Dawson‐type polyoxometalates (POMs) selectively hydrolyze the protein myoglobin at AspX peptide bonds under mildly acidic neutral conditions. This transformation is first example of highly sequence selective hydrolysis by POMs, a novel class protein‐hydrolyzing agents. The selectivity directed Asp residues located on surface further assisted electrostatic interactions between...
Several acute and chronic inflammatory diseases are driven by accumulation of activated leukocytes due to enhanced chemokine expression. In addition specific G protein-coupled receptor (GPCR)-dependent signaling, – glycosaminoglycan (GAG) interactions important for activity in vivo. Therefore, the GAG interaction has been explored as target inhibition activity. It was demonstrated that CXCL9(74-103) binds with high affinity GAGs, competed active chemokines binding thereby inhibited CXCL8-...
// Rik Janssens 1, 2 , Anneleen Mortier 1 Daiane Boff Vincent Vanheule Mieke Gouwy Charlotte Franck 3 Olav Larsen Mette M. Rosenkilde Jo Van Damme Flávio A. Amaral Mauro Teixeira Sofie Struyf Paul Proost Laboratory of Molecular Immunology, Department Microbiology and Rega Institute, KU Leuven, B-3000 Belgium Departamento de Bioquímica e Imunologia, Instituto Ciências Biológicas, Universidade Federal Minas Gerais, Belo Horizonte, Brasil for Pharmacology, Neuroscience Faculty Health Medical...
Matrix metalloproteinases (MMPs) are enzymes with critical roles in biology and pathology. Glycosylation, nitrosylation proteolysis known posttranslational modifications (PTMs) regulating intrinsically the activities of MMPs. We discovered MMP citrullination by peptidyl arginine deiminases (PADs) as a new PTM. Upon hypercitrullination, MMP-9 acquired higher affinity for gelatin than control MMP-9. Furthermore, hypercitrullinated proMMP-9 was more efficiently activated MMP-3 compared to...
CXC chemokine ligand (CXCL)9, CXCL10 and CXCL11 direct chemotaxis of mainly T cells NK through activation their common receptor (CXCR)3. They are inactivated upon NH₂-terminal cleavage by dipeptidyl peptidase IV/CD26. In the present study, we found that different glycosaminoglycans (GAGs) protect CXCR3 ligands against proteolytic processing CD26 without directly affecting enzymatic activity CD26. addition, GAGs were shown to interfere with chemokine-induced signaling. The observation heparan...
Abstract This study investigates if treatment with a peptide corresponding to the 30 C-terminal amino acids of CXCL9, CXCL9(74–103), ameliorates joint inflammation in murine model antigen-induced arthritis (AIA). AIA was induced male C57BL/6J mice. Intravenous injection simultaneously performed tibiofemoral challenge methylated BSA (mBSA) as antigen mice immunized mBSA, diminished accumulation leukocytes, particular neutrophils, synovial cavity. The levels chemokines CXCL1, CXCL2, and CXCL6...
Indoleamine 2,3-dioxygenase-1 (IDO1) is an immune-modulatory enzyme that catalyzes the degradation of tryptophan (Trp) to kynurenine (Kyn) and strongly induced by interferon (IFN)-γ. We previously reported highly increased levels IFN-γ corresponding IDO activity in patients with hemophagocytic lymphohistiocytosis (HLH), a hyper-inflammatory syndrome. On other hand, were low systemic juvenile idiopathic arthritis (sJIA), autoinflammatory As HLH can occur as complication sJIA, opposing both...
Abstract Sodefrin, a decapeptide isolated from the male dorsal gland of Japanese fire belly newt Cynops pyrrhogaster , was first peptide pheromone identified vertebrate. The salamander and sodefrin have become model for sex investigation in aquatically courting salamanders ever since. Subsequent studies other SPF protein courtship pheromones around 20 kDa belonging to same gene-family. Although transcripts these proteins could be PCR-amplified it is currently unknown whether they effectively...
Proteolytic processing of CXCL5 potentiates its chemoattractant activity toward neutrophils and monocytes in vitro vivo.
With ELISAs one detects the ensemble of immunoreactive molecules in biological samples. For biomolecules undergoing proteolysis for activation, potentiation or inhibition, other techniques are necessary to study biology. Here we develop methodology that combines immunosorbent sample preparation and nano-scale liquid chromatography-tandem mass spectrometry (nano-LC-MS/MS) proteoform analysis (ISTAMPA) apply this aglycosyl chemokine CXCL8. CXCL8, most powerful human with neutrophil chemotactic...
Hepatic cell death occurs in response to diverse stimuli such as chemical and physical damage. The exposure of intracellular contents DNA during necrosis induces a severe inflammatory that has yet be fully explored therapeutically. Here, we sought means neutralize the ability extracellular induce deleterious tissue inflammation when drug‐induced liver injury had already ensued. were investigated vivo using intravital microscopy. necrotic debris was studied murine livers models vitro by...
Objectives To explore posttranslational modifications (PTMs), including proteolytic activation, multimerization, complex formation and citrullination of gelatinases, in particular gelatinase B/MMP-9, to detect gelatin-Sepharose affinity-purified synovial fluids, the presence specific MMP proteoforms relation arthritis. Methods Latent, activated, complexed truncated gelatinase-A/MMP-2 B/MMP-9 were detected with use zymography analysis compare levels, substrate conversion assays, test net...
Abstract SDS‐PAGE/Edman degradation and HPLC MS/MS showed that zirconium(IV)‐substituted Lindqvist‐, Keggin‐, Wells–Dawson‐type polyoxometalates (POMs) selectively hydrolyze the protein myoglobin at AspX peptide bonds under mildly acidic neutral conditions. This transformation is first example of highly sequence selective hydrolysis by POMs, a novel class protein‐hydrolyzing agents. The selectivity directed Asp residues located on surface further assisted electrostatic interactions between...
CXCL12 is a chemotactic cytokine that attracts many different cell types for homeostasis and during inflammation. Under stress conditions, macrophages granulocytes produce factors such as peroxynitrite consequence of their oxidative response. After short incubations with peroxynitrite, the gradual nitration Tyr7, Tyr61 or both Tyr7 was demonstrated use mass spectrometry, whereas longer caused degradation. Native nitrated forms, [3-NT61]CXCL12 [3-NT7/61]CXCL12, were chemically synthesized to...