A5047145116- Phagocytosis and Immune Regulation
- Liver physiology and pathology
- SARS-CoV-2 and COVID-19 Research
- Chemokine receptors and signaling
- Hepatitis B Virus Studies
- Drug-Induced Hepatotoxicity and Protection
- Organ Transplantation Techniques and Outcomes
- Immunotherapy and Immune Responses
- Autophagy in Disease and Therapy
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- T-cell and B-cell Immunology
- Immune responses and vaccinations
- Liver Disease and Transplantation
- Disaster Response and Management
- Drug Transport and Resistance Mechanisms
- Immune cells in cancer
- interferon and immune responses
- Receptor Mechanisms and Signaling
- Glycosylation and Glycoproteins Research
- Pancreatic function and diabetes
- Erythrocyte Function and Pathophysiology
- Mosquito-borne diseases and control
- Immune Response and Inflammation
KU Leuven
2021-2025
Rega Institute for Medical Research
2021-2025
University of Copenhagen
2022
Our understanding of the functional heterogeneity resident versus recruited macrophages in diseased liver is limited. A population lipid-associated (LAMs) has been reported to populate alongside Kupffer cells (KCs). However, precise roles these distinct macrophage subsets remain elusive. Here, using proteogenomics, we have identified LAMs multiple models injury. Moreover, found that this phenotype not specific macrophages, as a subset KCs can also adopt LAM-like mouse and human liver. By...
<h2>Abstract</h2><h3>Background & aims</h3> Hepatocellular necrosis is common in both acute and chronic liver injury may evolve to fibrosis failure. Injury leads accumulation of necrotic cell debris the liver, which drives persistent inflammation poor recovery. This study investigated role natural antibodies (NAbs) clearance cells injured its impact on tissue regeneration potential NAbs as a therapy for injury. <h3>Methods</h3> We used murine models drug-induced focal thermal immunocompetent...
Necrotic cell death causes loss of membrane integrity, release intracellular contents and deposition necrotic debris. Effective clearance this debris is crucial for resolving inflammation promoting tissue recovery. While leukocyte phagocytosis plays a major role, soluble factors in the bloodstream also contribute to removal. Our study examined whether enzymatic degradation or disassembly enhances improves outcomes mouse model drug-induced liver injury. Using intravital microscopy proteomic...
Hepatic cell death occurs in response to diverse stimuli such as chemical and physical damage. The exposure of intracellular contents DNA during necrosis induces a severe inflammatory that has yet be fully explored therapeutically. Here, we sought means neutralize the ability extracellular induce deleterious tissue inflammation when drug‐induced liver injury had already ensued. were investigated vivo using intravital microscopy. necrotic debris was studied murine livers models vitro by...
Extensive ligand-receptor promiscuity in the chemokine signaling system balances beneficial redundancy and specificity. However, this feature poses a major challenge to selectively modulate pharmacologically. Here, we identified conserved cluster of three aromatic receptor residues that anchors second extracellular loop (ECL2) top transmembrane helices (TM) 4 5 enables recognition both shared specific characteristics interacting chemokines. This was essential for activation several...
ABSTRACT Usutu virus (USUV) is an emerging neurotropic flavivirus capable of causing encephalitis in humans. Here, our main goal was to characterize the innate immune response brain during USUV and identify strategies control disease severity. Using immunocompetent mouse model encephalitis, we showed that microglia activation, blood-brain barrier (BBB) disruption inflammatory monocyte recruitment are hallmarks 6 days post infection. Activated were close association USUV-infected cells,...
Abstract Cells die by necrosis due to excessive chemical or thermal stress, leading plasma membrane rupture, release of intracellular components and severe inflammation. The clearance necrotic cell debris is crucial for tissue recovery injury resolution, however, the underlying mechanisms are still poorly understood, especially in vivo. This study examined role complement proteins promoting leukocytes their influence on liver regeneration. We found that independently type injury, either...
Cells die by necrosis due to excessive chemical or thermal stress, leading plasma membrane rupture, release of intracellular components and severe inflammation. The clearance necrotic cell debris is crucial for tissue recovery injury resolution, however, the underlying mechanisms are still poorly understood, especially in vivo . This study examined role complement proteins promoting leukocytes their influence on liver regeneration. We found that independently type injury, either...
Natural antibodies (NAbs) are circulating polyreactive immunoglobulins that bind endogenous and exogenous antigens. Here, we investigated the role of NAbs in driving clearance necrotic cell debris from injury sites. Using mouse models liver injury, observed IgM IgG opsonize vivo by recognizing common self-molecules such as histones, actin, phosphoinositides cardiolipin, but not phosphatidylserine. Importantly, mice lacking presented impaired recovery which was correlated to sustained...
Inflammatory chemokines are often elevated in disease settings, where the largest group of CC‐chemokines macrophage inflammatory proteins (MIP), which promiscuous for receptors CCR1 and CCR5. MIP chemokines, such as CCL3 CCL5 processed at N terminus, influences signaling a highly diverse manner. Here, we investigate capacity peptides corresponding to truncated termini. These 3–10‐residue displayed weak potency but, surprisingly, retained their on CCR1. In contrast, none generated signal...
Summary Natural antibodies (NAbs) are circulating polyreactive immunoglobulins that bind endogenous and exogenous antigens. Here, we investigated the role of NAbs in driving clearance necrotic cell debris from injury sites. Using mouse models liver injury, observed IgM IgG opsonize vivo by recognizing common self-molecules such as histones, actin, phosphoinositides cardiolipin, but not phosphatidylserine. Importantly, mice lacking presented impaired recovery which was correlated to sustained...